FastQCFastQC Report
Fri 10 Feb 2017
ERR1631903.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631903.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181080
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74490.3415280503236928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70870.3249307682432557No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA60930.2793570157903424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60060.27536816622957433No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA54600.2503346965723403No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA54170.2483631962147193No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA54150.24827149852366717No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC46090.211317329029655No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA45640.20925413098098192No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG45280.20760357254204337No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA42980.19705833807104736No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT41840.19183156968107543No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC40290.18472499862453462No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA36790.16867790269041028No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA36690.16821941423514955No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT35370.16216736662570838No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC31930.1463953637647404No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT31700.1453408403176408No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA31210.1430942468868634No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG30820.1413061419113467No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA30170.13832596695215216No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT28290.12970638399325105No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC26770.12273735947328845No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA26650.12218717332697564No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC25830.11842756799383791No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA25600.1173730445467383No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT25390.11641021879069086No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA25330.11613512571753443No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG25240.11572248610779981No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA25200.11553909072569551No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC24620.11287985768518348No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC24600.11278815999413135No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT24500.11232967153887066No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA24390.11182533423808388No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA23930.1097162873438847No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC23140.10609422854732518No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC23110.10595668201074697No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA22840.10471876318154309No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC22440.1028848093605003No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA22200.10178443706787463No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG22140.10150934399471821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41600.029.706731
CTTATAC21400.019.71028137
GCGGTAA21550.019.05800423
CGTTATT2950.018.81355910
GTATCAA65700.018.7252672
CGAAAAT609.2404394E-418.537
TCTTATA41350.018.0302337
AGCCGCG23000.018.01739119
CCGCTTA2600.017.78846225
TATACAC6250.017.76000237
CGCGGTA23350.017.66809322
TTCTGCG17500.017.44285818
TGCGGGT17100.017.41812921
TGTTACG3300.017.37878816
ATACGGC3000.017.26666629
CTCTATG12950.017.1428571
GGTAATA24300.017.12963125
CGGTAAT24200.017.12396624
TTACACT650.00158054917.0769234
CGTGCCA24850.017.0482910
Produced by FastQC (version 0.11.5)