FastQCFastQC Report
Fri 10 Feb 2017
ERR1631901.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631901.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249973
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6050.2420261388229929No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5490.21962371936169103No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5120.20482212078904521No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4990.19962155912838586No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4230.16921827557376196No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3890.15561680661511443No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3660.14641581290779404No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3450.13801490560980584No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3290.1316142143351482No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3230.12921395510715158No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3150.12601360946982273No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3140.12561356626515666No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3030.12121309101382949No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2990.11961291819516508No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2800.1120120973065091No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2510.10041084437119208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2700.032.888891
ATACGGC801.8189894E-1230.062529
GCCTGTT405.921832E-527.7511
TTACCCC405.921832E-527.754
TGTTTAC358.8499085E-426.42857214
CTAGCAT358.8499085E-426.42857235
TACGGCT1002.0008883E-1124.05000130
TTAGGAC400.001926984823.1253
TTAGAAC400.001926984823.1254
TTATGAG400.001926984823.1253
TAGCATC400.001926984823.12536
GTATTAG502.6939125E-422.21
GCGGTAA2400.021.58333423
CTTACCC603.7112884E-521.5833343
CTATCCT450.00381756320.55555517
ACCTCTA450.00381756320.55555531
CCAAAAA450.00381756320.55555520
TATCCTA450.00381756320.55555518
GGTAATA2450.020.38775425
GTCCTAA555.1276333E-420.1818181
Produced by FastQC (version 0.11.5)