##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631901.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	249973
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9510547139091	33.0	31.0	34.0	30.0	34.0
2	32.143347481528004	33.0	31.0	34.0	30.0	34.0
3	32.23761766270757	34.0	31.0	34.0	30.0	34.0
4	35.85508034867766	37.0	35.0	37.0	35.0	37.0
5	35.58733143179463	37.0	35.0	37.0	33.0	37.0
6	35.61060194501006	37.0	35.0	37.0	33.0	37.0
7	35.628195845151275	37.0	35.0	37.0	33.0	37.0
8	35.58532321490721	37.0	35.0	37.0	33.0	37.0
9	37.34534129686006	39.0	37.0	39.0	35.0	39.0
10	37.172654646701844	39.0	37.0	39.0	34.0	39.0
11	37.278814111924085	39.0	37.0	39.0	34.0	39.0
12	37.180811527644984	39.0	37.0	39.0	34.0	39.0
13	37.26657679029335	39.0	37.0	39.0	34.0	39.0
14	38.44477203537982	40.0	38.0	41.0	34.0	41.0
15	38.427798202205835	40.0	38.0	41.0	34.0	41.0
16	38.34348509639041	40.0	38.0	41.0	34.0	41.0
17	38.31459797658147	40.0	38.0	41.0	34.0	41.0
18	38.323522940477574	40.0	38.0	41.0	34.0	41.0
19	38.35141795313894	40.0	38.0	41.0	34.0	41.0
20	38.321538726182425	40.0	38.0	41.0	34.0	41.0
21	38.27198137398839	40.0	38.0	41.0	34.0	41.0
22	38.23605349377733	40.0	38.0	41.0	34.0	41.0
23	38.1750429046337	40.0	38.0	41.0	34.0	41.0
24	38.184619938953404	40.0	38.0	41.0	34.0	41.0
25	38.09838662575558	40.0	38.0	41.0	34.0	41.0
26	37.997035679853425	40.0	37.0	41.0	33.0	41.0
27	37.85908078072432	40.0	37.0	41.0	33.0	41.0
28	37.85908478115636	40.0	37.0	41.0	33.0	41.0
29	37.81073555944042	40.0	37.0	41.0	33.0	41.0
30	37.75303332759938	40.0	37.0	41.0	33.0	41.0
31	37.67163653674597	39.0	37.0	41.0	33.0	41.0
32	37.62939197433323	39.0	37.0	40.0	33.0	41.0
33	37.5610685954083	39.0	37.0	40.0	33.0	41.0
34	37.51264336548347	39.0	37.0	40.0	32.0	41.0
35	37.412212518952046	39.0	36.0	40.0	32.0	41.0
36	37.36395930760522	39.0	36.0	40.0	32.0	41.0
37	37.293155660811365	39.0	36.0	40.0	32.0	41.0
38	37.25435947082285	39.0	36.0	40.0	32.0	41.0
39	37.16340964824201	39.0	36.0	40.0	32.0	41.0
40	36.867257663827694	39.0	35.0	40.0	31.0	41.0
41	36.91840318754426	39.0	35.0	40.0	31.0	41.0
42	36.89247638744985	39.0	35.0	40.0	31.0	41.0
43	36.33548823272913	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	4.0
18	4.0
19	13.0
20	28.0
21	56.0
22	113.0
23	196.0
24	326.0
25	540.0
26	770.0
27	1079.0
28	1661.0
29	2337.0
30	3193.0
31	4334.0
32	5859.0
33	7943.0
34	11102.0
35	16732.0
36	26531.0
37	45972.0
38	75718.0
39	45459.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.76261836278318	16.74700867693711	12.694571013669476	27.795801946610233
2	21.39311045592924	20.136174706868342	31.874642461385825	26.596072375816586
3	21.41671300500454	20.103371164085722	28.925523956587313	29.554391874322427
4	15.99852784100683	16.25175518956047	33.710840770803244	34.03887619862945
5	17.04344069159469	32.86594952254844	33.63083212987002	16.459777655986844
6	34.44291983534222	33.909262200317634	15.290851411952492	16.356966552387657
7	30.73171902565477	27.33215187240222	20.004160449328527	21.931968652614483
8	27.23734163289635	30.92694010953183	20.42060542538594	21.415112832185876
9	28.381865241446075	12.679369371892166	17.3938785388822	41.54488684777956
10	19.32928756305681	23.478935725058307	29.972437023198506	27.21933968868638
11	39.143027446964275	19.50890696195189	18.816432174674866	22.531633416408972
12	22.81846439415457	23.73536341924928	26.71128481876043	26.734887367835725
13	34.82776139823101	17.54589495665532	21.023070491613094	26.603273153500577
14	24.53584987178615	19.70372800262428	23.05208962567957	32.70833249990999
15	29.493185264008513	23.982990162937597	19.938553363763287	26.5852712092906
16	26.021610333916062	23.961787873090294	22.217199457541415	27.79940233545223
17	27.340152736495543	23.62335132194277	21.69994359390814	27.336552347653548
18	26.53606589511667	21.789953314958016	23.307717233461215	28.366263556464098
19	28.298256211670857	22.242802222640044	23.14209934672945	26.316842218959646
20	29.802418661215412	20.922259604037237	22.707652426462058	26.567669308285296
21	27.654986738567764	22.129589995719538	21.79755413584667	28.417869129866023
22	28.408668136158706	22.85446828257452	21.03587187416241	27.700991707104368
23	27.962619962955998	22.276005808627332	22.059582434902968	27.701791793513696
24	27.837006396690846	22.04118044748833	22.348813671876563	27.772999483944265
25	28.353462173914785	22.07078364463362	22.66324763074412	26.912506550707477
26	28.021426314041914	22.901273337520454	22.290407363995314	26.786892984442318
27	27.586579350569863	21.721145883755447	23.362123109295805	27.330151656378888
28	26.594072159793257	22.838466554387875	21.999575954203053	28.567885331615816
29	27.35175398943086	23.275713777087926	22.09558632332292	27.276945910158297
30	27.00931700623667	22.09758653934625	23.514139527068924	27.378956927348153
31	28.102635084589135	22.583639033015565	22.462825985206404	26.850899897188896
32	25.850791885523638	22.324811079596596	22.93767726914507	28.886719765734696
33	26.28603889220036	22.10358718741624	24.0762002296248	27.5341736907586
34	26.885303612790178	22.00837690470571	23.41892924435839	27.68739023814572
35	26.220831849839783	23.507738835794264	24.06379889028015	26.207630424085803
36	26.406451896804857	22.43122257203778	24.384633540422364	26.777691990734997
37	27.058122277205936	22.11798874278422	23.986190508574925	26.83769847143492
38	26.14802398659055	21.793953747004675	24.96869661923488	27.089325647169893
39	25.62556756129662	21.151484360310914	25.534357710632747	27.68859036775972
40	25.35393822532834	21.997175694975056	26.30284070679633	26.346045372900274
41	24.13140619186872	20.95906357886652	27.246942669808337	27.66258755945642
42	22.865269449100502	21.5411264416557	28.041028431070558	27.55257567817324
43	22.342412980601907	21.934368911842476	27.898213007004756	27.82500510055086
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	5.5
4	10.0
5	10.0
6	13.5
7	17.0
8	15.0
9	13.0
10	18.0
11	23.0
12	23.0
13	53.5
14	84.0
15	196.5
16	309.0
17	299.0
18	289.0
19	289.0
20	353.5
21	418.0
22	368.5
23	319.0
24	401.0
25	483.0
26	483.0
27	577.0
28	671.0
29	933.5
30	1196.0
31	1602.0
32	2008.0
33	2008.0
34	2503.5
35	2999.0
36	3555.5
37	4112.0
38	5062.5
39	6013.0
40	6013.0
41	7225.0
42	8437.0
43	9677.0
44	10917.0
45	14414.5
46	17912.0
47	17912.0
48	19610.5
49	21309.0
50	24511.0
51	27713.0
52	28720.5
53	29728.0
54	29728.0
55	27226.0
56	24724.0
57	23318.0
58	21912.0
59	20416.5
60	18921.0
61	18921.0
62	17264.0
63	15607.0
64	13101.0
65	10595.0
66	9128.5
67	7662.0
68	7662.0
69	6481.5
70	5301.0
71	4647.5
72	3994.0
73	3211.5
74	2429.0
75	2429.0
76	1969.5
77	1510.0
78	1260.5
79	1011.0
80	797.0
81	583.0
82	583.0
83	466.0
84	349.0
85	278.0
86	207.0
87	159.0
88	111.0
89	111.0
90	82.5
91	54.0
92	35.5
93	17.0
94	9.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	249973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.93265767207633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.77063634537832	39.194839195708624
2	8.500246704800176	8.148788308587635
3	2.9082855021479617	4.182055601613626
4	1.5354012456968829	2.9438344919707307
5	0.9695763114066747	2.323718471080531
6	0.688753971009998	1.9808284987623257
7	0.4673912542678976	1.5682313492821867
8	0.39599362050251147	1.5184821321498394
9	0.3029101675366847	1.3067360429334478
>10	2.127220909012618	19.767775631510993
>50	0.23261790070167426	7.682583239600451
>100	0.09799647731687554	8.591663618001789
>500	0.002969590221723501	0.7904634187978231
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	605	0.2420261388229929	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	549	0.21962371936169103	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	512	0.20482212078904521	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	499	0.19962155912838586	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	423	0.16921827557376196	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	389	0.15561680661511443	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	366	0.14641581290779404	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	345	0.13801490560980584	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	329	0.1316142143351482	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	323	0.12921395510715158	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	315	0.12601360946982273	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	314	0.12561356626515666	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	303	0.12121309101382949	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	299	0.11961291819516508	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	280	0.1120120973065091	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	251	0.10041084437119208	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	4.0004320466610393E-4	0.0	0.0	0.0	0.0
18	4.0004320466610393E-4	0.0	0.0	0.0	0.0
19	4.0004320466610393E-4	0.0	0.0	4.0004320466610393E-4	0.0
20	4.0004320466610393E-4	0.0	0.0	4.0004320466610393E-4	0.0
21	4.0004320466610393E-4	0.0	0.0	0.0028003024326627274	0.0
22	4.0004320466610393E-4	0.0	0.0	0.005600604865325455	0.0
23	4.0004320466610393E-4	0.0	0.0	0.0068007344793237666	0.0
24	4.0004320466610393E-4	0.0	0.0	0.008800950502654286	0.0
25	4.0004320466610393E-4	0.0	0.0	0.010001080116652598	0.0
26	4.0004320466610393E-4	0.0	0.0	0.01320142575398143	0.0
27	4.0004320466610393E-4	0.0	0.0	0.0304032835546239	0.0
28	4.0004320466610393E-4	0.0	0.0	0.08280894336588351	0.0
29	4.0004320466610393E-4	0.0	0.0	0.18481996055574001	0.0
30	4.0004320466610393E-4	0.0	0.0	0.33123577346353406	0.0
31	4.0004320466610393E-4	0.0	0.0	0.6992755217563497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	270	0.0	32.88889	1
ATACGGC	80	1.8189894E-12	30.0625	29
GCCTGTT	40	5.921832E-5	27.75	11
TTACCCC	40	5.921832E-5	27.75	4
TGTTTAC	35	8.8499085E-4	26.428572	14
CTAGCAT	35	8.8499085E-4	26.428572	35
TACGGCT	100	2.0008883E-11	24.050001	30
TTAGGAC	40	0.0019269848	23.125	3
TTAGAAC	40	0.0019269848	23.125	4
TTATGAG	40	0.0019269848	23.125	3
TAGCATC	40	0.0019269848	23.125	36
GTATTAG	50	2.6939125E-4	22.2	1
GCGGTAA	240	0.0	21.583334	23
CTTACCC	60	3.7112884E-5	21.583334	3
CTATCCT	45	0.003817563	20.555555	17
ACCTCTA	45	0.003817563	20.555555	31
CCAAAAA	45	0.003817563	20.555555	20
TATCCTA	45	0.003817563	20.555555	18
GGTAATA	245	0.0	20.387754	25
GTCCTAA	55	5.1276333E-4	20.181818	1
>>END_MODULE