FastQCFastQC Report
Fri 10 Feb 2017
ERR1631899.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631899.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068371
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA25260.23643472164631948No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA25220.23606031987015744No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA24510.22941468834328152No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA23770.22248825548428403No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA21330.19964974713840042No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC20850.19515692582445612No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG20370.1906641045105118No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT18830.1762496361282738No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC18720.17522003124382823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.1619287681900763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17200.16099276374967122No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16930.15846555176057756No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT16640.15575113888340286No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA16540.1548151344429978No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA14630.13693744963126106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13900.13010461721630406No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC12350.11559654839002557No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA12300.11512854616982304No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12270.11484774483770152No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA11830.11072932529991922No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11760.11007412219163568No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11650.1090445173071901No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT11510.107734111090623No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC11340.10614290354193441No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11250.10530049954556986No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11100.10389649288496225No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11100.10389649288496225No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA11090.10380289244092175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16750.027.05971
ATACAGT603.7259702E-521.5833326
TATTAGA909.476753E-820.5555552
ATACGGC1800.020.55555529
GCGGTAA9700.020.40721723
TACCTTA1101.7535058E-920.18181831
CGCGGTA10200.019.76960822
GTATAGA951.675835E-719.4736841
TTCGCTA1153.0504452E-919.30434826
CGGTAAT10450.019.11961724
GGTAATA10650.018.93427325
CCGCGGT11100.018.66666621
TCGCTAC1205.171387E-918.527
GTATCAA24350.018.4620132
TAATACG10300.018.32038927
CGTGCCA11500.018.01739110
AGCCGCG11450.017.77292619
CTTATAC10350.017.51690737
AACTCCG11500.017.3739135
TATACAC3100.017.30645237
Produced by FastQC (version 0.11.5)