##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631899.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1068371
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53993884146986	34.0	31.0	34.0	31.0	34.0
2	32.708971883362615	34.0	31.0	34.0	31.0	34.0
3	32.80256296735872	34.0	31.0	34.0	31.0	34.0
4	36.24475205710376	37.0	37.0	37.0	35.0	37.0
5	36.15603006820665	37.0	35.0	37.0	35.0	37.0
6	36.203448053157565	37.0	36.0	37.0	35.0	37.0
7	36.200463134997115	37.0	36.0	37.0	35.0	37.0
8	36.17926450643082	37.0	36.0	37.0	35.0	37.0
9	38.00450686138055	39.0	38.0	39.0	35.0	39.0
10	37.92236498369948	39.0	38.0	39.0	35.0	39.0
11	38.00820595092903	39.0	38.0	39.0	35.0	39.0
12	37.93129633806983	39.0	38.0	39.0	35.0	39.0
13	37.996738960529626	39.0	38.0	39.0	35.0	39.0
14	39.35881636622484	40.0	39.0	41.0	36.0	41.0
15	39.36604232050477	40.0	39.0	41.0	36.0	41.0
16	39.31000935068436	40.0	39.0	41.0	36.0	41.0
17	39.28232421134606	40.0	39.0	41.0	36.0	41.0
18	39.31019280755468	40.0	39.0	41.0	36.0	41.0
19	39.325435639866676	40.0	39.0	41.0	36.0	41.0
20	39.32024175122687	40.0	39.0	41.0	36.0	41.0
21	39.279965480156235	40.0	39.0	41.0	36.0	41.0
22	39.25059740483409	40.0	39.0	41.0	36.0	41.0
23	39.18523902277392	40.0	39.0	41.0	36.0	41.0
24	39.197258255793166	40.0	39.0	41.0	36.0	41.0
25	39.137424171940275	40.0	39.0	41.0	36.0	41.0
26	39.07296248213402	40.0	39.0	41.0	36.0	41.0
27	38.95562683749372	40.0	38.0	41.0	35.0	41.0
28	38.951261312783664	40.0	38.0	41.0	35.0	41.0
29	38.91448101829795	40.0	38.0	41.0	35.0	41.0
30	38.8743966281376	40.0	38.0	41.0	35.0	41.0
31	38.78226196705077	40.0	38.0	41.0	35.0	41.0
32	38.73548327313265	40.0	38.0	41.0	35.0	41.0
33	38.66314604196482	40.0	38.0	41.0	35.0	41.0
34	38.63800215468222	40.0	38.0	41.0	35.0	41.0
35	38.570504066471294	40.0	38.0	41.0	35.0	41.0
36	38.51747754291346	40.0	38.0	41.0	35.0	41.0
37	38.46671147007921	40.0	38.0	41.0	34.0	41.0
38	38.42861983337249	40.0	38.0	41.0	34.0	41.0
39	38.33832442101105	40.0	38.0	41.0	34.0	41.0
40	38.03393016096469	40.0	37.0	41.0	34.0	41.0
41	38.09177336337284	40.0	37.0	41.0	34.0	41.0
42	38.061412187339414	40.0	37.0	41.0	34.0	41.0
43	37.57024666525018	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	0.0
13	1.0
14	0.0
15	1.0
16	1.0
17	2.0
18	4.0
19	10.0
20	26.0
21	79.0
22	152.0
23	318.0
24	555.0
25	904.0
26	1524.0
27	2445.0
28	3776.0
29	5505.0
30	7864.0
31	10721.0
32	14412.0
33	19729.0
34	28696.0
35	42729.0
36	66107.0
37	119480.0
38	271806.0
39	471521.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.13857264938865	16.358081602739123	12.655060835608603	27.848284912263622
2	20.8921807125053	19.345246173847848	32.60505947840217	27.157513635244683
3	21.3580301224949	19.690070209693076	29.184618451829934	29.767281215982088
4	15.456054123520763	16.192408816787427	34.21339590835019	34.13814115134162
5	17.111565177265202	32.52072547832167	33.29264834032373	17.075061004089402
6	33.59226336169739	33.755970538324235	15.89747381761579	16.754292282362588
7	30.310350992305107	27.854462541570296	20.05501834100701	21.780168125117587
8	27.436442958485397	30.45299806902284	20.198695022609186	21.91186394988258
9	28.586605214855137	12.928842134427084	17.949289151427735	40.53526349929004
10	19.495662087420943	23.374464488459534	29.64410303162478	27.48577039249474
11	39.11843357784889	19.491169266106997	19.12556593168478	22.264831224359327
12	22.655893879560566	23.731550182474066	27.073460436496312	26.539095501469056
13	35.12010340977058	17.633387652791026	20.78744181562397	26.459067121814428
14	23.84873793841278	20.239036813990648	23.762719130339555	32.14950611725702
15	29.648221451162566	24.110538380394075	20.250175266831466	25.991064901611892
16	25.107851111645672	24.2217357079142	23.041714909895532	27.628698270544593
17	26.545647532551897	23.91285424258053	22.193414085556423	27.34808413931116
18	26.732099617080586	21.48176990951645	23.631772109126885	28.154358364276078
19	27.84220088340099	22.58859516029544	23.681567545356437	25.887636410947135
20	29.70279051003818	21.019851718176554	23.001653919846195	26.275703851939074
21	27.356601779718844	22.37022532434894	22.432844021412038	27.840328874520182
22	28.160067991362553	22.857509236023816	22.01697724854007	26.965445524073566
23	27.92176126083542	22.060969457239104	22.809398607786996	27.20787067413848
24	27.639181520277134	21.770152877605252	23.327102663775037	27.263562938342577
25	27.525550581211956	22.094010413985405	23.595923139059373	26.78451586574327
26	27.591726095148594	22.801442570043555	22.956632106262713	26.65019922854514
27	26.760179750292735	22.014824438327135	24.579102203260852	26.645893608119277
28	26.332987323691864	23.0622134071404	22.46532337549409	28.139475893673637
29	27.04921792148982	23.496706668376437	22.749868725377233	26.704206684756514
30	26.811379193182894	22.04168776576676	23.995971436888496	27.15096160416185
31	27.654438392655734	22.394187038023308	23.086643123034975	26.864731446285983
32	25.722712428547762	22.395123042463712	23.2717848013471	28.610379727641426
33	25.664867354130728	22.081655155372058	25.02782273199104	27.225654758506174
34	26.20887313489415	22.15017068040971	24.150880171775533	27.490076012920607
35	25.59588382687287	23.485755416423697	24.75011021452286	26.168250542180573
36	26.24350529918914	22.273348864767016	25.015935475597896	26.467210360445947
37	26.494260888773656	22.277186482972674	24.674761857070248	26.553790771183415
38	25.683306641606706	21.694055716600317	25.42684142493572	27.195796216857254
39	25.316018499191763	20.774992956566585	26.44951987652229	27.45946866771936
40	25.13480803952934	21.682730062871418	26.890752369729242	26.29170952787
41	23.44569442637436	21.17513485483975	27.841358479404626	27.537812239381264
42	22.16495955056811	21.691715705499305	28.644637490160253	27.49868725377233
43	21.69265170993971	22.238623100027986	28.174950461964993	27.89377472806731
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	8.5
2	9.0
3	33.0
4	57.0
5	57.0
6	87.0
7	117.0
8	142.0
9	167.0
10	242.5
11	318.0
12	318.0
13	679.5
14	1041.0
15	1999.0
16	2957.0
17	2899.0
18	2841.0
19	2841.0
20	3234.0
21	3627.0
22	2979.0
23	2331.0
24	2513.0
25	2695.0
26	2695.0
27	3119.0
28	3543.0
29	4421.5
30	5300.0
31	6622.5
32	7945.0
33	7945.0
34	10355.0
35	12765.0
36	14636.0
37	16507.0
38	20480.0
39	24453.0
40	24453.0
41	29492.0
42	34531.0
43	41332.0
44	48133.0
45	65503.5
46	82874.0
47	82874.0
48	91780.5
49	100687.0
50	111927.5
51	123168.0
52	122718.0
53	122268.0
54	122268.0
55	111254.5
56	100241.0
57	94646.5
58	89052.0
59	83614.0
60	78176.0
61	78176.0
62	71472.0
63	64768.0
64	54184.5
65	43601.0
66	37694.5
67	31788.0
68	31788.0
69	27226.0
70	22664.0
71	18864.0
72	15064.0
73	12232.5
74	9401.0
75	9401.0
76	7691.5
77	5982.0
78	4911.0
79	3840.0
80	3148.0
81	2456.0
82	2456.0
83	1971.5
84	1487.0
85	1161.0
86	835.0
87	603.0
88	371.0
89	371.0
90	298.0
91	225.0
92	143.0
93	61.0
94	37.5
95	14.0
96	14.0
97	8.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1068371.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.40433671916484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.05376463480675	42.51429163969752
2	7.305759222504996	7.218723772355615
3	2.202686986145923	3.2646686865142667
4	1.1171048240175296	2.2075929150546165
5	0.6461493596046088	1.596129026638941
6	0.442465097623883	1.3115816801693074
7	0.2853659789513235	0.9868821838613704
8	0.2186943665925334	0.8643560100577596
9	0.17926769285012215	0.7970941314391728
>10	1.245734324669486	12.250370283108051
>50	0.1611370127292328	5.591398353424722
>100	0.12222982558653635	12.081794636006245
>500	0.013729403126548537	4.764436443116357
>1k	0.005911270790597287	4.5506802385560885
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2526	0.23643472164631948	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2522	0.23606031987015744	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2451	0.22941468834328152	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2377	0.22248825548428403	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2133	0.19964974713840042	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2085	0.19515692582445612	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2037	0.1906641045105118	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1883	0.1762496361282738	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1872	0.17522003124382823	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1730	0.1619287681900763	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1720	0.16099276374967122	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1693	0.15846555176057756	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1664	0.15575113888340286	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1654	0.1548151344429978	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1463	0.13693744963126106	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1390	0.13010461721630406	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1235	0.11559654839002557	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1230	0.11512854616982304	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1227	0.11484774483770152	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	1183	0.11072932529991922	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1176	0.11007412219163568	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1165	0.1090445173071901	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	1151	0.107734111090623	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1134	0.10614290354193441	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1125	0.10530049954556986	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1110	0.10389649288496225	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1110	0.10389649288496225	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	1109	0.10380289244092175	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	9.360044404050652E-5	0.0	9.360044404050652E-5	0.0	0.0
9	9.360044404050652E-5	0.0	9.360044404050652E-5	0.0	0.0
10	1.8720088808101305E-4	0.0	9.360044404050652E-5	0.0	0.0
11	1.8720088808101305E-4	9.360044404050652E-5	9.360044404050652E-5	9.360044404050652E-5	0.0
12	1.8720088808101305E-4	9.360044404050652E-5	9.360044404050652E-5	9.360044404050652E-5	0.0
13	2.808013321215196E-4	9.360044404050652E-5	9.360044404050652E-5	9.360044404050652E-5	0.0
14	2.808013321215196E-4	9.360044404050652E-5	9.360044404050652E-5	9.360044404050652E-5	0.0
15	3.744017761620261E-4	9.360044404050652E-5	9.360044404050652E-5	9.360044404050652E-5	0.0
16	3.744017761620261E-4	9.360044404050652E-5	9.360044404050652E-5	2.808013321215196E-4	0.0
17	4.6800222020253266E-4	9.360044404050652E-5	9.360044404050652E-5	4.6800222020253266E-4	0.0
18	4.6800222020253266E-4	9.360044404050652E-5	9.360044404050652E-5	6.552031082835457E-4	0.0
19	4.6800222020253266E-4	9.360044404050652E-5	9.360044404050652E-5	8.424039963645587E-4	0.0
20	4.6800222020253266E-4	9.360044404050652E-5	9.360044404050652E-5	0.001591207548688611	0.0
21	4.6800222020253266E-4	9.360044404050652E-5	9.360044404050652E-5	0.0028080133212151958	0.0
22	4.6800222020253266E-4	9.360044404050652E-5	9.360044404050652E-5	0.005148024422227859	0.0
23	4.6800222020253266E-4	9.360044404050652E-5	9.360044404050652E-5	0.006364830194754444	0.0
24	6.552031082835457E-4	9.360044404050652E-5	9.360044404050652E-5	0.008517640407686095	0.0
25	6.552031082835457E-4	9.360044404050652E-5	9.360044404050652E-5	0.009640845736172172	0.0
26	6.552031082835457E-4	9.360044404050652E-5	9.360044404050652E-5	0.012823260833549394	0.0
27	6.552031082835457E-4	9.360044404050652E-5	9.360044404050652E-5	0.029764941204881077	0.0
28	6.552031082835457E-4	9.360044404050652E-5	9.360044404050652E-5	0.10867011553102808	0.0
29	6.552031082835457E-4	9.360044404050652E-5	9.360044404050652E-5	0.2351243154297524	0.0
30	7.488035523240522E-4	9.360044404050652E-5	9.360044404050652E-5	0.4038859160347857	0.0
31	7.488035523240522E-4	9.360044404050652E-5	9.360044404050652E-5	0.8980026601246196	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1675	0.0	27.0597	1
ATACAGT	60	3.7259702E-5	21.583332	6
TATTAGA	90	9.476753E-8	20.555555	2
ATACGGC	180	0.0	20.555555	29
GCGGTAA	970	0.0	20.407217	23
TACCTTA	110	1.7535058E-9	20.181818	31
CGCGGTA	1020	0.0	19.769608	22
GTATAGA	95	1.675835E-7	19.473684	1
TTCGCTA	115	3.0504452E-9	19.304348	26
CGGTAAT	1045	0.0	19.119617	24
GGTAATA	1065	0.0	18.934273	25
CCGCGGT	1110	0.0	18.666666	21
TCGCTAC	120	5.171387E-9	18.5	27
GTATCAA	2435	0.0	18.462013	2
TAATACG	1030	0.0	18.320389	27
CGTGCCA	1150	0.0	18.017391	10
AGCCGCG	1145	0.0	17.772926	19
CTTATAC	1035	0.0	17.516907	37
AACTCCG	1150	0.0	17.373913	5
TATACAC	310	0.0	17.306452	37
>>END_MODULE