FastQCFastQC Report
Fri 10 Feb 2017
ERR1631897.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631897.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677412
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA23800.35133714785093856No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA23140.3415941849273411No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA22450.33140836005267105No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA21950.3240273275347942No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG18900.2790030291757453No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA18780.27723158137145487No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC18420.2719172379585836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18240.2692600662521479No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17680.26099330983212576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.25656469032139967No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC16300.24062166008278568No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16070.2372263851245623No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA15600.23028821455775805No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA15010.22157859618666337No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT14710.21714997667593725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12870.1899877770101504No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11880.1753733326247542No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11780.17389712612117886No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11400.16828754140759242No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11100.1638589218968663No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11060.16326843929543616No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA10750.1586921991343525No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC10620.15677313067970453No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10500.15500168287541408No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10370.1530826144207661No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA10330.15249213181933596No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA10270.1516064079171907No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC10210.1507206840150455No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT10160.14998258076325782No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC9490.14009199718930282No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA9450.13950151458787266No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT9370.13832054938501237No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9320.13758244613322468No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG9130.13477765377643147No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA9090.1341871711750013No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9000.1328585853217835No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC8880.13108713751749304No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG8860.13079189621677798No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC8650.1276918625592697No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA8650.1276918625592697No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC8630.12739662125855464No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC8360.1234108636989011No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT8340.12311562239818603No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC8330.1229680017478285No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG8290.12237751914639834No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA8170.1206060713421079No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8100.11957272678960515No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC7960.11750603768459962No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA7880.11632507248173932No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA7690.11352028012494612No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA7660.11307741817387351No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT7490.11056786711779538No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC7480.11042024646743782No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA7410.10938690191493508No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA7130.10525352370492404No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC7080.10451542045313633No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6970.10289159329920344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACT200.00184140337.04
TTCTATA250.005494769729.62
CATATAA250.005494769729.62
CTTTTAC250.005494769729.621
ATATAAC250.005494769729.63
ACATATA250.005494769729.61
GGTATCA14450.029.5743941
TAAACCA1000.025.9000025
TATATGT451.3224283E-424.66666827
CTACCGA451.3224283E-424.6666684
GCAGTCG3050.023.6557399
CAGTCGG3100.023.27419510
CTACTGT400.001930373623.12534
AGTCGGT3200.023.12511
GGGCAGT3150.022.9047627
TCACAAT1700.022.85294233
CCGCTTA652.6801463E-622.7692325
CCTCACT652.6801463E-622.769238
CAGTATT652.6801463E-622.7692314
TGCACCG1155.456968E-1222.5217412
Produced by FastQC (version 0.11.5)