##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631897.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	677412
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.594150974591535	34.0	31.0	34.0	31.0	34.0
2	32.759134765844124	34.0	31.0	34.0	31.0	34.0
3	32.840233122531046	34.0	31.0	34.0	31.0	34.0
4	36.25485376698376	37.0	37.0	37.0	35.0	37.0
5	36.19156436555597	37.0	35.0	37.0	35.0	37.0
6	36.257453366636554	37.0	37.0	37.0	35.0	37.0
7	36.25300998506079	37.0	37.0	37.0	35.0	37.0
8	36.237096478952246	37.0	37.0	37.0	35.0	37.0
9	38.074546952224054	39.0	38.0	39.0	37.0	39.0
10	38.00881885765236	39.0	38.0	39.0	35.0	39.0
11	38.077912703052206	39.0	38.0	39.0	37.0	39.0
12	38.00437252366359	39.0	38.0	39.0	35.0	39.0
13	38.06666253328846	39.0	38.0	39.0	35.0	39.0
14	39.41764096295903	40.0	39.0	41.0	37.0	41.0
15	39.43647588173815	40.0	39.0	41.0	37.0	41.0
16	39.372668331827605	40.0	39.0	41.0	36.0	41.0
17	39.34547217941223	40.0	39.0	41.0	36.0	41.0
18	39.39427556642043	40.0	39.0	41.0	37.0	41.0
19	39.40271356279487	40.0	39.0	41.0	37.0	41.0
20	39.406070456384	40.0	39.0	41.0	37.0	41.0
21	39.353014118439	40.0	39.0	41.0	36.0	41.0
22	39.31792764226202	40.0	39.0	41.0	36.0	41.0
23	39.25697507572939	40.0	39.0	41.0	36.0	41.0
24	39.25583544430863	40.0	39.0	41.0	36.0	41.0
25	39.19750019190685	40.0	39.0	41.0	36.0	41.0
26	39.127821768731586	40.0	39.0	41.0	35.0	41.0
27	38.978699816359914	40.0	39.0	41.0	35.0	41.0
28	38.979036391442726	40.0	39.0	41.0	35.0	41.0
29	38.95424055080217	40.0	38.0	41.0	35.0	41.0
30	38.900125772794105	40.0	38.0	41.0	35.0	41.0
31	38.787331786268915	40.0	38.0	41.0	35.0	41.0
32	38.720064008314	40.0	38.0	41.0	35.0	41.0
33	38.64306212467449	40.0	38.0	41.0	35.0	41.0
34	38.623154003767276	40.0	38.0	41.0	35.0	41.0
35	38.528697454429505	40.0	38.0	41.0	35.0	41.0
36	38.47101173288929	40.0	38.0	41.0	35.0	41.0
37	38.40991449811931	40.0	38.0	41.0	34.0	41.0
38	38.3613103989891	40.0	38.0	41.0	34.0	41.0
39	38.254247046110784	40.0	37.0	41.0	34.0	41.0
40	37.91570565623284	40.0	37.0	41.0	33.0	41.0
41	37.973283614698296	40.0	37.0	41.0	33.0	41.0
42	37.94118940910406	40.0	37.0	41.0	33.0	41.0
43	37.446543610092526	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	2.0
16	1.0
17	1.0
18	3.0
19	16.0
20	30.0
21	65.0
22	119.0
23	213.0
24	416.0
25	590.0
26	1017.0
27	1642.0
28	2380.0
29	3491.0
30	4870.0
31	6746.0
32	9148.0
33	12279.0
34	18108.0
35	27240.0
36	41273.0
37	74064.0
38	162908.0
39	310789.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.217421598672594	16.493802885097992	12.848163303868251	27.440612212361167
2	21.616239452504534	19.826634308220108	32.2554073444226	26.301718894852765
3	21.858337319090893	19.82176282675831	29.72755132770013	28.592348526450667
4	14.962829120239974	16.814139696373847	36.03346264902304	32.18956853436313
5	16.73117689087291	32.3621370746311	33.91171104143416	16.994974993061827
6	31.031927394259327	35.074961766251555	16.34529651083831	17.54781432865081
7	28.831936841980948	28.088224005479677	20.429812285581008	22.650026866958363
8	26.692765997649882	29.544796962557495	21.105472002267454	22.656965037525172
9	28.77953151110402	12.326619546155072	18.403866480074164	40.48998246266674
10	20.414164496643107	22.448524679220327	28.790603059880844	28.346707764255726
11	38.63306230181928	19.801095935708254	18.80436130449416	22.761480457978305
12	22.18930281719249	24.67626791376592	26.707970924636705	26.426458344404878
13	35.89661830614161	17.7386878295631	20.42420270086742	25.940491163427872
14	23.635837570045997	21.309336120411213	24.1823292176696	30.872497091873186
15	30.65756142495261	23.480983507820945	20.615666684381146	25.245788382845298
16	24.135976333457336	24.286992258773097	23.529993563739644	28.047037844029926
17	26.36608149840865	23.893140363619185	21.803570057808248	27.937208080163916
18	26.51089735640939	20.66689105005521	23.674218939138957	29.147992654396436
19	28.06814759703105	22.22310794612437	24.155314638654172	25.55342981819041
20	30.60397512887283	19.92849255696681	23.280071802684336	26.18746051147603
21	27.409169013835005	21.893913895827062	22.390362142979455	28.306554947358475
22	28.28721664216164	23.044469244713707	21.97953387303443	26.688780240090225
23	27.972637036249726	21.645763582576038	23.149427527117915	27.23217185405632
24	27.48681747592307	21.415327747367925	23.69872396709831	27.399130809610696
25	27.445631314473317	22.04005833968102	23.935064628320728	26.579245717524934
26	27.5176701918478	23.05672175869338	22.879576978264335	26.546031071194488
27	26.51060211510868	21.800027162199665	25.347794252242355	26.341576470449297
28	25.710055328219756	23.19578041133018	22.506244353510123	28.58791990693994
29	27.102265681741688	23.678647558649686	22.742289773431825	26.476796986176804
30	26.647889319941186	21.578300945362646	24.76321647682651	27.010593257869658
31	27.86206916913193	22.501372872048325	23.25025243131211	26.38630552750763
32	25.08650570110952	22.33264246868966	23.729429062372677	28.851422767828144
33	25.27826492592396	21.801946230654316	25.810584991113238	27.109203852308493
34	25.738546113738757	22.03607258212137	24.840717318264215	27.384663985875658
35	25.071891256724122	24.247577545127633	24.89873223385473	25.781798964293518
36	26.010758592998055	22.904524868174757	25.223202423340595	25.86151411548659
37	26.591645852154965	22.92548700052553	24.598767072328215	25.884100074991288
38	25.8176707823304	21.89037100021848	25.18349246839442	27.108465749056705
39	25.25523610446818	21.04214274326407	26.320909579399242	27.381711572868504
40	25.265126688042137	22.233146150348677	26.796690935501587	25.705036226107598
41	23.116950984039256	21.332069700566272	27.730096307712294	27.82088300768218
42	21.41252295501113	22.14693569053988	28.99018617916423	27.450355175284763
43	21.037566503102987	22.971839884737797	28.03006146923881	27.960532142920407
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	13.5
2	12.0
3	35.0
4	58.0
5	58.0
6	100.0
7	142.0
8	146.0
9	150.0
10	258.0
11	366.0
12	366.0
13	694.5
14	1023.0
15	1990.5
16	2958.0
17	2755.0
18	2552.0
19	2552.0
20	2893.0
21	3234.0
22	2265.5
23	1297.0
24	1154.0
25	1011.0
26	1011.0
27	1008.0
28	1005.0
29	1220.5
30	1436.0
31	2027.5
32	2619.0
33	2619.0
34	4346.5
35	6074.0
36	6706.0
37	7338.0
38	10345.0
39	13352.0
40	13352.0
41	17118.5
42	20885.0
43	26047.0
44	31209.0
45	44782.0
46	58355.0
47	58355.0
48	64737.5
49	71120.0
50	80462.5
51	89805.0
52	87948.0
53	86091.0
54	86091.0
55	76154.5
56	66218.0
57	61071.5
58	55925.0
59	51189.0
60	46453.0
61	46453.0
62	41594.0
63	36735.0
64	28748.5
65	20762.0
66	17502.5
67	14243.0
68	14243.0
69	11828.5
70	9414.0
71	7980.0
72	6546.0
73	5464.5
74	4383.0
75	4383.0
76	3913.5
77	3444.0
78	3315.5
79	3187.0
80	3005.0
81	2823.0
82	2823.0
83	2699.0
84	2575.0
85	1867.5
86	1160.0
87	1052.5
88	945.0
89	945.0
90	615.0
91	285.0
92	235.5
93	186.0
94	102.0
95	18.0
96	18.0
97	10.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	677412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.3213449832639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.43492394784515	23.291388087940984
2	8.245513709495295	4.835391040806871
3	3.184015384257424	2.8007884054149454
4	1.7715551561709804	2.0777751956387704
5	1.1936977224375462	1.7500411362663841
6	0.8312687807575221	1.462435121664508
7	0.62554069235787	1.28391861091864
8	0.48490674691918156	1.1374494408903648
9	0.4221479995832521	1.114015241679775
>10	2.963279459147572	17.700471994496535
>50	0.45076312830408327	9.20515111173933
>100	0.34213327531186866	19.810885478037214
>500	0.035533129483428495	7.104425069778609
>1k	0.014720867928848946	6.425864064727056
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2380	0.35133714785093856	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2314	0.3415941849273411	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2245	0.33140836005267105	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2195	0.3240273275347942	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1890	0.2790030291757453	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1878	0.27723158137145487	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1842	0.2719172379585836	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1824	0.2692600662521479	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1768	0.26099330983212576	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1738	0.25656469032139967	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1630	0.24062166008278568	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1607	0.2372263851245623	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1560	0.23028821455775805	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1501	0.22157859618666337	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1471	0.21714997667593725	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1287	0.1899877770101504	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1188	0.1753733326247542	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1178	0.17389712612117886	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1140	0.16828754140759242	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1110	0.1638589218968663	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1106	0.16326843929543616	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1075	0.1586921991343525	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	1062	0.15677313067970453	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1050	0.15500168287541408	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1037	0.1530826144207661	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	1033	0.15249213181933596	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	1027	0.1516064079171907	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1021	0.1507206840150455	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	1016	0.14998258076325782	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	949	0.14009199718930282	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	945	0.13950151458787266	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	937	0.13832054938501237	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	932	0.13758244613322468	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	913	0.13477765377643147	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	909	0.1341871711750013	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	900	0.1328585853217835	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	888	0.13108713751749304	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	886	0.13079189621677798	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	865	0.1276918625592697	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	865	0.1276918625592697	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	863	0.12739662125855464	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	836	0.1234108636989011	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	834	0.12311562239818603	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	833	0.1229680017478285	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	829	0.12237751914639834	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	817	0.1206060713421079	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	810	0.11957272678960515	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	796	0.11750603768459962	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	788	0.11632507248173932	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	769	0.11352028012494612	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	766	0.11307741817387351	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	749	0.11056786711779538	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	748	0.11042024646743782	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	741	0.10938690191493508	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	713	0.10525352370492404	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	708	0.10451542045313633	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	697	0.10289159329920344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4762065035753722E-4
2	0.0	0.0	0.0	0.0	1.4762065035753722E-4
3	0.0	0.0	0.0	0.0	1.4762065035753722E-4
4	0.0	0.0	0.0	0.0	1.4762065035753722E-4
5	0.0	0.0	0.0	0.0	1.4762065035753722E-4
6	0.0	0.0	0.0	0.0	1.4762065035753722E-4
7	0.0	0.0	0.0	0.0	1.4762065035753722E-4
8	1.4762065035753722E-4	0.0	0.0	0.0	1.4762065035753722E-4
9	1.4762065035753722E-4	0.0	0.0	0.0	1.4762065035753722E-4
10	1.4762065035753722E-4	0.0	0.0	0.0	1.4762065035753722E-4
11	1.4762065035753722E-4	0.0	0.0	0.0	1.4762065035753722E-4
12	1.4762065035753722E-4	0.0	0.0	0.0	1.4762065035753722E-4
13	1.4762065035753722E-4	0.0	0.0	0.0	1.4762065035753722E-4
14	2.9524130071507444E-4	0.0	0.0	0.0	2.9524130071507444E-4
15	2.9524130071507444E-4	0.0	0.0	4.4286195107261163E-4	2.9524130071507444E-4
16	2.9524130071507444E-4	0.0	0.0	7.381032517876861E-4	4.4286195107261163E-4
17	2.9524130071507444E-4	0.0	0.0	0.0010333445525027604	4.4286195107261163E-4
18	2.9524130071507444E-4	0.0	0.0	0.0011809652028602978	4.4286195107261163E-4
19	2.9524130071507444E-4	0.0	0.0	0.0017714478042904465	4.4286195107261163E-4
20	5.904826014301489E-4	0.0	0.0	0.0025095510560781326	4.4286195107261163E-4
21	5.904826014301489E-4	0.0	0.0	0.005461964063228877	4.4286195107261163E-4
22	5.904826014301489E-4	0.0	0.0	0.008709618371094695	5.904826014301489E-4
23	5.904826014301489E-4	0.0	0.0	0.012104893329318052	5.904826014301489E-4
24	5.904826014301489E-4	0.0	0.0	0.015057306336468797	5.904826014301489E-4
25	5.904826014301489E-4	0.0	0.0	0.017271616091831855	5.904826014301489E-4
26	5.904826014301489E-4	0.0	0.0	0.02229071820398812	5.904826014301489E-4
27	5.904826014301489E-4	0.0	0.0	0.04280998860368579	5.904826014301489E-4
28	5.904826014301489E-4	0.0	0.0	0.12710137995783954	5.904826014301489E-4
29	5.904826014301489E-4	0.0	0.0	0.2800363737282481	5.904826014301489E-4
30	5.904826014301489E-4	0.0	0.0	0.4726813224448342	5.904826014301489E-4
31	5.904826014301489E-4	0.0	0.0	0.9595342273239919	5.904826014301489E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACT	20	0.001841403	37.0	4
TTCTATA	25	0.0054947697	29.6	2
CATATAA	25	0.0054947697	29.6	2
CTTTTAC	25	0.0054947697	29.6	21
ATATAAC	25	0.0054947697	29.6	3
ACATATA	25	0.0054947697	29.6	1
GGTATCA	1445	0.0	29.574394	1
TAAACCA	100	0.0	25.900002	5
TATATGT	45	1.3224283E-4	24.666668	27
CTACCGA	45	1.3224283E-4	24.666668	4
GCAGTCG	305	0.0	23.655739	9
CAGTCGG	310	0.0	23.274195	10
CTACTGT	40	0.0019303736	23.125	34
AGTCGGT	320	0.0	23.125	11
GGGCAGT	315	0.0	22.904762	7
TCACAAT	170	0.0	22.852942	33
CCGCTTA	65	2.6801463E-6	22.76923	25
CCTCACT	65	2.6801463E-6	22.76923	8
CAGTATT	65	2.6801463E-6	22.76923	14
TGCACCG	115	5.456968E-12	22.52174	12
>>END_MODULE