FastQCFastQC Report
Fri 10 Feb 2017
ERR1631896.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631896.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045596
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29680.2838572450544952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28910.27649302407430787No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA23340.2232219710098355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22670.21681414236473742No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA20740.19835577029751456No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20150.19271305552048784No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA18990.1816189044334523No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17990.17205498108255962No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16720.1599087984269259No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA16680.1595262414928902No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC16100.15397916594937242No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT15420.14747569807076538No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14630.13992019862356014No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA14450.13819869242039945No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13550.12959116140459603No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC12850.12289641505897114No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT12270.1173493395154534No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12160.11629730794685518No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12070.11543655484527486No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11840.11323685247456954No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11770.11256737784000703No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10930.10453368222525718No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10920.10443804299174823No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC10630.10166450521998935No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10590.10128194828595365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTACG751.4551915E-1129.64
GGTATCA17750.028.9746481
CTAGAAC400.001931071223.1253
GTATCAA26000.019.6384622
TACCGCT951.675744E-719.47368435
TAGCGAA1153.0486262E-919.30434810
CTTATAC8250.018.83636337
GCCTTAA500.00703446718.51
TCGGGTA1103.8506187E-818.525
CTACCGT1205.171387E-918.58
GCTACCG1205.171387E-918.57
CTCTATG5000.018.131
AGTCGGT3600.017.9861111
TTACGAG1258.574716E-917.766
CCGCTTA1054.7962385E-717.61904725
GCGGTAA8650.017.53757323
CAGTCGG3800.017.52631610
CGCGGTA8800.017.44886422
TCTTATA15400.017.41883337
GTCCTAA2450.017.3673461
Produced by FastQC (version 0.11.5)