##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631896.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1045596
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60033894544355	34.0	31.0	34.0	31.0	34.0
2	32.76422729237679	34.0	31.0	34.0	31.0	34.0
3	32.85479190815573	34.0	31.0	34.0	31.0	34.0
4	36.269202445303925	37.0	37.0	37.0	35.0	37.0
5	36.19927581972387	37.0	35.0	37.0	35.0	37.0
6	36.255268765374005	37.0	37.0	37.0	35.0	37.0
7	36.245704842023116	37.0	37.0	37.0	35.0	37.0
8	36.23136565174312	37.0	37.0	37.0	35.0	37.0
9	38.05884777677038	39.0	38.0	39.0	35.0	39.0
10	37.99095061572539	39.0	38.0	39.0	35.0	39.0
11	38.07070895451015	39.0	38.0	39.0	37.0	39.0
12	38.00270467752363	39.0	38.0	39.0	35.0	39.0
13	38.06167678529757	39.0	38.0	39.0	35.0	39.0
14	39.43741272919942	40.0	39.0	41.0	37.0	41.0
15	39.44212965619608	40.0	39.0	41.0	37.0	41.0
16	39.39692290330108	40.0	39.0	41.0	37.0	41.0
17	39.37004254033106	40.0	39.0	41.0	36.0	41.0
18	39.39630411746028	40.0	39.0	41.0	37.0	41.0
19	39.41167621146217	41.0	39.0	41.0	37.0	41.0
20	39.40998148424439	40.0	39.0	41.0	37.0	41.0
21	39.37280651417947	40.0	39.0	41.0	36.0	41.0
22	39.34202502687462	40.0	39.0	41.0	36.0	41.0
23	39.27725622515771	40.0	39.0	41.0	36.0	41.0
24	39.29090107460243	40.0	39.0	41.0	36.0	41.0
25	39.23659520503139	40.0	39.0	41.0	36.0	41.0
26	39.1743837964185	40.0	39.0	41.0	36.0	41.0
27	39.05709566601249	40.0	39.0	41.0	35.0	41.0
28	39.04964728250682	40.0	39.0	41.0	35.0	41.0
29	39.01575369454359	40.0	39.0	41.0	35.0	41.0
30	38.97193275414214	40.0	38.0	41.0	35.0	41.0
31	38.872762520132056	40.0	38.0	41.0	35.0	41.0
32	38.81087437212843	40.0	38.0	41.0	35.0	41.0
33	38.73937352476482	40.0	38.0	41.0	35.0	41.0
34	38.701486998802594	40.0	38.0	41.0	35.0	41.0
35	38.62370647936679	40.0	38.0	41.0	35.0	41.0
36	38.56233095765477	40.0	38.0	41.0	35.0	41.0
37	38.49942042624494	40.0	38.0	41.0	35.0	41.0
38	38.458417017662654	40.0	38.0	41.0	35.0	41.0
39	38.370754096228374	40.0	38.0	41.0	34.0	41.0
40	38.03671972731342	40.0	37.0	41.0	34.0	41.0
41	38.088910057039236	40.0	37.0	41.0	34.0	41.0
42	38.05963106209282	40.0	37.0	41.0	34.0	41.0
43	37.58334002807968	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	1.0
17	5.0
18	7.0
19	21.0
20	25.0
21	65.0
22	155.0
23	298.0
24	467.0
25	903.0
26	1439.0
27	2285.0
28	3565.0
29	5091.0
30	7617.0
31	9980.0
32	13559.0
33	18596.0
34	26845.0
35	40261.0
36	61652.0
37	114215.0
38	249324.0
39	489218.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.14572741288222	17.03631230417867	12.77233271741667	27.04562756552244
2	20.69231328352442	20.094854991794154	32.7748002096412	26.43803151504023
3	21.353276026304616	20.41696793025222	29.461283325490918	28.76847271795225
4	15.255796693942978	16.333172659421038	34.60810867677382	33.80292196986217
5	16.865500633131724	32.99754398448349	33.2204790377928	16.916476344591985
6	33.134499366868276	34.232437767550756	15.898970539290511	16.734092326290458
7	29.624730775557673	28.398635801973228	20.46746544554493	21.509167976924164
8	27.277743985248605	30.56907256722482	20.356810852375105	21.79637259515147
9	28.248099648430173	13.131553678476198	18.580981564581347	40.03936510851228
10	19.15299982019824	23.864762298249037	30.122532985971638	26.859704895581082
11	38.10592236389581	20.096672137230822	19.633873886281126	22.163531612592244
12	22.43925952279848	24.624902926177988	27.49828805772019	25.43754949330334
13	34.6432082754716	18.204258623789684	21.366856797462884	25.785676303275835
14	23.593146875083683	20.80009869968898	24.38676123474076	31.219993190486573
15	29.10081905439577	24.79236722405212	20.863220593804872	25.24359312774724
16	24.6432656590117	24.447492148018927	23.37709784658702	27.53214434638235
17	25.74053458505962	24.074977333501657	23.066365976916515	27.118122104522207
18	26.23431994766621	21.83749746556031	24.51654367461237	27.411638912161102
19	27.27611811827895	23.025145467274164	24.690989636532656	25.007746777914225
20	28.77191573035857	21.72435625231925	24.03127020378808	25.4724578135341
21	26.8844754570599	22.975126148149	23.277154847570188	26.863243547220918
22	27.14193627366593	23.58291347709823	22.979142995956373	26.29600725327947
23	26.987574550782522	22.594386359549958	23.75755071748553	26.66048837218199
24	26.904272778396244	22.411906702014928	24.076029365070255	26.607791154518573
25	26.718350108454892	22.872983446761463	24.334542213244887	26.074124231538757
26	26.708021071235926	23.352040367407678	23.65244319985922	26.287495361497175
27	26.086174774960885	22.697198535572056	25.135233876181623	26.081392813285436
28	25.671674336933194	23.49808147697581	23.486891686655266	27.34335249943573
29	26.088661395032116	23.955428291615497	23.72713744122969	26.22877287212269
30	26.06522978282243	23.01682485395889	24.77276118118279	26.14518418203589
31	26.990348088554278	22.996644975688508	23.8376007559325	26.175406179824712
32	24.752103106744862	23.21384167498728	24.114571976174354	27.919483242093506
33	25.035960351799357	22.84199633510457	25.72819712393697	26.393846189159103
34	25.21499699692807	22.779830833323768	25.013676410391778	26.991495759356386
35	25.055853312369216	24.246267200716147	25.22303069254282	25.474848794371823
36	25.654554914135097	23.251906089923832	25.256026228103394	25.837512767837673
37	25.86343100011859	23.209824827179908	24.967195742906437	25.959548429795063
38	25.16574279167097	22.559191121618674	25.751724375380164	26.52334171133019
39	24.80279190050459	21.712401348130637	26.72093236775963	26.763874383605142
40	24.677791422308427	22.544749597358827	27.08474401202759	25.692714968305157
41	23.293509156500217	22.019403287694292	27.89834697148803	26.78874058431746
42	22.075352239297015	22.434955757290577	28.66594745963068	26.82374454378173
43	21.57563724421287	23.01873763862907	28.149208680981946	27.25641643617611
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	27.5
2	36.0
3	90.5
4	145.0
5	145.0
6	179.0
7	213.0
8	248.0
9	283.0
10	434.0
11	585.0
12	585.0
13	1250.0
14	1915.0
15	3619.0
16	5323.0
17	5195.5
18	5068.0
19	5068.0
20	5847.5
21	6627.0
22	5528.0
23	4429.0
24	4782.0
25	5135.0
26	5135.0
27	5800.5
28	6466.0
29	7769.0
30	9072.0
31	10545.0
32	12018.0
33	12018.0
34	14781.5
35	17545.0
36	19159.5
37	20774.0
38	24371.5
39	27969.0
40	27969.0
41	32994.0
42	38019.0
43	43339.5
44	48660.0
45	63484.0
46	78308.0
47	78308.0
48	86407.0
49	94506.0
50	104056.0
51	113606.0
52	113298.0
53	112990.0
54	112990.0
55	104503.5
56	96017.0
57	90708.5
58	85400.0
59	79402.0
60	73404.0
61	73404.0
62	66180.0
63	58956.0
64	48390.0
65	37824.0
66	32689.5
67	27555.0
68	27555.0
69	23525.0
70	19495.0
71	16477.5
72	13460.0
73	10850.0
74	8240.0
75	8240.0
76	6836.0
77	5432.0
78	4450.5
79	3469.0
80	3005.0
81	2541.0
82	2541.0
83	2106.5
84	1672.0
85	1388.5
86	1105.0
87	916.5
88	728.0
89	728.0
90	575.0
91	422.0
92	280.5
93	139.0
94	79.0
95	19.0
96	19.0
97	13.0
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1045596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.2432332680604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.32263014266101	41.644827822848974
2	6.834612843234887	6.594476433861245
3	2.1627999570405	3.130213885189676
4	1.0512554708970958	2.0286385162725313
5	0.6580920697961657	1.587424461751856
6	0.4580092561560849	1.3257508430201301
7	0.31624569450207735	1.067970036691844
8	0.2652994145485327	1.0239121233555777
9	0.20659418060377027	0.8970094122022342
>10	1.392875848382678	13.3476027811619
>50	0.1824242453327687	6.125790353032642
>100	0.1288479136835877	12.178922784616079
>500	0.014537708928855655	4.752820647655793
>1k	0.00577525423201115	4.294639898339632
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2968	0.2838572450544952	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2891	0.27649302407430787	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2334	0.2232219710098355	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2267	0.21681414236473742	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2074	0.19835577029751456	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2015	0.19271305552048784	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1899	0.1816189044334523	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1799	0.17205498108255962	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1672	0.1599087984269259	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1668	0.1595262414928902	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1610	0.15397916594937242	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1542	0.14747569807076538	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1463	0.13992019862356014	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1445	0.13819869242039945	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1355	0.12959116140459603	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1285	0.12289641505897114	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1227	0.1173493395154534	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1216	0.11629730794685518	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1207	0.11543655484527486	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1184	0.11323685247456954	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1177	0.11256737784000703	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	1093	0.10453368222525718	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1092	0.10443804299174823	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	1063	0.10166450521998935	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	1059	0.10128194828595365	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	9.563923350892697E-5	0.0	0.0	0.0	0.0
9	9.563923350892697E-5	0.0	0.0	0.0	0.0
10	9.563923350892697E-5	0.0	0.0	0.0	0.0
11	9.563923350892697E-5	0.0	0.0	0.0	0.0
12	9.563923350892697E-5	0.0	0.0	0.0	0.0
13	1.9127846701785393E-4	0.0	0.0	0.0	0.0
14	1.9127846701785393E-4	0.0	0.0	0.0	0.0
15	1.9127846701785393E-4	0.0	0.0	9.563923350892697E-5	0.0
16	2.869177005267809E-4	0.0	0.0	5.738354010535617E-4	0.0
17	2.869177005267809E-4	0.0	0.0	6.694746345624888E-4	0.0
18	2.869177005267809E-4	0.0	0.0	0.0010520315685981967	0.0
19	2.869177005267809E-4	0.0	0.0	0.0014345885026339044	0.0
20	2.869177005267809E-4	0.0	0.0	0.0021040631371963933	0.0
21	2.869177005267809E-4	0.0	0.0	0.004112487040883859	0.0
22	3.8255693403570787E-4	0.0	0.0	0.0075554994472052306	0.0
23	3.8255693403570787E-4	0.0	0.0	0.008703170249312353	0.0
24	3.8255693403570787E-4	0.0	0.0	0.011285429554053383	0.0
25	3.8255693403570787E-4	0.0	0.0	0.012911296523705141	0.0
26	3.8255693403570787E-4	0.0	0.0	0.016258669696517583	9.563923350892697E-5
27	3.8255693403570787E-4	0.0	0.0	0.034525763296722634	9.563923350892697E-5
28	4.781961675446348E-4	0.0	0.0	0.10176014445349829	9.563923350892697E-5
29	4.781961675446348E-4	0.0	0.0	0.2071545797803358	9.563923350892697E-5
30	4.781961675446348E-4	0.0	0.0	0.35386516398302975	9.563923350892697E-5
31	4.781961675446348E-4	0.0	0.0	0.7416822558617286	1.9127846701785393E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTACG	75	1.4551915E-11	29.6	4
GGTATCA	1775	0.0	28.974648	1
CTAGAAC	40	0.0019310712	23.125	3
GTATCAA	2600	0.0	19.638462	2
TACCGCT	95	1.675744E-7	19.473684	35
TAGCGAA	115	3.0486262E-9	19.304348	10
CTTATAC	825	0.0	18.836363	37
GCCTTAA	50	0.007034467	18.5	1
TCGGGTA	110	3.8506187E-8	18.5	25
CTACCGT	120	5.171387E-9	18.5	8
GCTACCG	120	5.171387E-9	18.5	7
CTCTATG	500	0.0	18.13	1
AGTCGGT	360	0.0	17.98611	11
TTACGAG	125	8.574716E-9	17.76	6
CCGCTTA	105	4.7962385E-7	17.619047	25
GCGGTAA	865	0.0	17.537573	23
CAGTCGG	380	0.0	17.526316	10
CGCGGTA	880	0.0	17.448864	22
TCTTATA	1540	0.0	17.418833	37
GTCCTAA	245	0.0	17.367346	1
>>END_MODULE