FastQCFastQC Report
Fri 10 Feb 2017
ERR1631895.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631895.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507996
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7450.14665469806848871No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6830.134449877558091No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5800.11417412735533351No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5600.11023708848101166No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5140.10118189907007143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAC250.005493491429.63
GGTATCA5550.029.3333341
TACAACT400.001929711723.1254
CTGGTGT652.6779544E-622.769234
GCGGTAA3200.020.812523
TTTAGAG450.003822913620.5555553
CCTTTAG450.003822913620.5555551
CCGGTCT909.4521965E-820.5555559
GTATCAA7700.020.4220772
CCTAAAC555.137665E-420.1818183
GGACCGT555.137665E-420.1818186
CGCGGTA3500.019.0285722
CTTATAC4550.018.70329937
TAATACG3600.018.527
CGTGCCA3800.018.510
GCACCGA500.007029609318.56
TATTGTC609.227146E-418.55
TATACAC1205.153197E-918.537
CCGCGGT3700.018.021
CGGTAAT3700.018.024
Produced by FastQC (version 0.11.5)