FastQCFastQC Report
Fri 10 Feb 2017
ERR1631894.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631894.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775793
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18270.23550096481922367No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA17850.23008714953602316No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15870.20456487748664914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15790.20353367457556334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.2030180731200204No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14760.19025693709533342No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14750.1901280367314477No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13280.1711796832402458No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12780.16473466504595943No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11770.15171572829350097No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11680.15055562501852943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11410.1470753151936148No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11140.14359500536870015No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10890.14037249627155696No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10150.13083386934401314No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10090.1300604671606988No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9990.12877146352184154No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9880.12735355951909852No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9870.1272246591552128No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9770.12593565551635552No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9340.12039293986926924No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9340.12039293986926924No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC8950.11536582567772588No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC8630.11124101403338263No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC8420.10853410639178233No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8320.10724510275292506No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8260.10647170056961072No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8210.10582719875018207No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8170.10531159729463917No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT8140.10492489620298198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGG251.2332559E-436.99999613
GTATCGT251.2332559E-436.9999966
CGTCCGA251.2332559E-436.99999621
AATCGGT303.5984098E-430.83333214
GGTATCA13050.029.7701151
TAGGCCG250.005495256329.5999985
TTAGACT604.3190084E-827.7499984
GCGTACT451.3226707E-424.66666617
CGAACTA400.001930624623.12524
CCGTCCA400.001930624623.1252
GTCCGAC400.001930624623.12522
CGTGCGT502.7010054E-422.19999914
TAGACTC603.724277E-521.5833325
CCGCTTA705.0983963E-621.14285725
GTTAGAC806.954124E-720.81253
CTGACGT450.00382470420.5555554
GTCGTCC450.00382470420.55555519
TCGGTCG450.00382470420.55555516
TTAGATA1550.020.2903234
TACGGCT1101.7516868E-920.18181830
Produced by FastQC (version 0.11.5)