##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631894.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775793
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.499768623846826	33.0	31.0	34.0	31.0	34.0
2	32.675169794004326	34.0	31.0	34.0	31.0	34.0
3	32.7538183510292	34.0	31.0	34.0	31.0	34.0
4	36.21160283735481	37.0	37.0	37.0	35.0	37.0
5	36.12540716402442	37.0	35.0	37.0	35.0	37.0
6	36.184025893505094	37.0	36.0	37.0	35.0	37.0
7	36.177303739528455	37.0	36.0	37.0	35.0	37.0
8	36.16284885272231	37.0	36.0	37.0	35.0	37.0
9	37.97368241270545	39.0	38.0	39.0	35.0	39.0
10	37.89957372649663	39.0	38.0	39.0	35.0	39.0
11	37.97364374259629	39.0	38.0	39.0	35.0	39.0
12	37.90859932997591	39.0	38.0	39.0	35.0	39.0
13	37.95935127024864	39.0	38.0	39.0	35.0	39.0
14	39.30917912381267	40.0	39.0	41.0	36.0	41.0
15	39.31594123690211	40.0	39.0	41.0	36.0	41.0
16	39.26558244273924	40.0	39.0	41.0	36.0	41.0
17	39.24011688684997	40.0	39.0	41.0	36.0	41.0
18	39.26740638288822	40.0	39.0	41.0	36.0	41.0
19	39.28039825056426	40.0	39.0	41.0	36.0	41.0
20	39.28972290288775	40.0	39.0	41.0	36.0	41.0
21	39.239679914616396	40.0	39.0	41.0	36.0	41.0
22	39.217271875358506	40.0	39.0	41.0	36.0	41.0
23	39.149310447503396	40.0	39.0	41.0	36.0	41.0
24	39.16510718709759	40.0	39.0	41.0	36.0	41.0
25	39.108214433489344	40.0	39.0	41.0	36.0	41.0
26	39.0482731862752	40.0	39.0	41.0	35.0	41.0
27	38.92167884989939	40.0	38.0	41.0	35.0	41.0
28	38.920235165823875	40.0	38.0	41.0	35.0	41.0
29	38.887861839433974	40.0	38.0	41.0	35.0	41.0
30	38.84958100936719	40.0	38.0	41.0	35.0	41.0
31	38.758533526340145	40.0	38.0	41.0	35.0	41.0
32	38.707187355389905	40.0	38.0	41.0	35.0	41.0
33	38.63929940074221	40.0	38.0	41.0	35.0	41.0
34	38.61324734819726	40.0	38.0	41.0	35.0	41.0
35	38.54253389757319	40.0	38.0	41.0	35.0	41.0
36	38.48840476776666	40.0	38.0	41.0	35.0	41.0
37	38.440448676386616	40.0	38.0	41.0	34.0	41.0
38	38.40115211145241	40.0	38.0	41.0	34.0	41.0
39	38.31455040197579	40.0	38.0	41.0	34.0	41.0
40	37.994279401850754	40.0	37.0	41.0	33.0	41.0
41	38.06504054560946	40.0	37.0	41.0	34.0	41.0
42	38.03791346402971	40.0	37.0	41.0	34.0	41.0
43	37.568283034263004	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.0
17	2.0
18	11.0
19	21.0
20	28.0
21	70.0
22	141.0
23	266.0
24	432.0
25	730.0
26	1177.0
27	1883.0
28	2835.0
29	4089.0
30	5725.0
31	7859.0
32	10562.0
33	14510.0
34	21143.0
35	31589.0
36	49102.0
37	88333.0
38	201279.0
39	334002.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.51211341169616	17.39935781838712	13.193467845159725	26.89506092475699
2	20.46963558578126	20.40982581693828	33.610254281747835	25.510284315532623
3	21.182067896977674	20.72060459426677	30.310276065909335	27.787051442846224
4	15.191036784296841	16.830004911103863	35.18309652188148	32.795861782717814
5	16.462509973665657	33.47026848656794	33.651889099282926	16.41533244048348
6	31.963809933835442	34.9472088559706	16.3474019487157	16.741579261478254
7	28.71951667519557	29.107120069399954	20.650224995585162	21.52313825981931
8	26.351101389159222	31.537407530101458	20.786472680212377	21.325018400526943
9	28.086229187424994	13.416723275409806	18.627391585126446	39.869655952038755
10	19.037423642646946	24.023289717746874	30.24969289488304	26.68959374472314
11	37.68208787653408	20.49283765128069	19.907758899603373	21.91731557258186
12	22.137606294462568	24.82827249021324	27.58802928100666	25.446091934317533
13	34.47930053506541	18.583694361769183	21.71674660637567	25.22025849678974
14	23.450585401002588	21.319862385971515	24.4420870000116	30.787465213014297
15	28.84506562962027	25.20543495494288	20.965644186013538	24.983855229423312
16	24.176165549315346	25.13724666244733	23.592118000549114	27.094469787688208
17	25.786131094248077	24.687255492122254	22.789455434632693	26.737157978996972
18	25.800052333547736	22.018244557504385	24.621516306540535	27.560186802407344
19	27.299034665174858	23.33341497023046	24.724765498013003	24.642784866581678
20	28.936713788343027	21.64133989350252	24.049456491615675	25.37248982653878
21	26.444940854068033	23.395416045259495	23.20644811180302	26.953194988869456
22	26.95628859760271	23.909857397527436	22.816524511048694	26.317329493821163
23	26.845434284660985	22.940526661106762	23.767422495433703	26.446616558798546
24	26.813466994417322	22.618662452484102	24.21586686139215	26.35200369170642
25	26.631846381702335	23.10178101632781	24.45665274113069	25.80971986083917
26	26.732646466260974	23.65618148140032	23.55215888774454	26.059013164594163
27	26.092785059932222	22.948002882212137	25.20479045312345	25.75442160473219
28	25.48102393293056	23.90663488843029	23.35906614264372	27.25327503599543
29	26.019698553609018	24.376476714793764	23.61364436131803	25.990180370279187
30	25.719360705755275	23.380850304140406	25.04791871027452	25.851870279829797
31	26.961057911066483	23.251563239163023	23.92957915320195	25.857799696568545
32	24.33625980126142	23.37595209031275	24.304679212109413	27.983108896316416
33	24.831108298218727	22.944006970931678	25.76769834221242	26.457186388637176
34	25.021751936405717	23.058728294789976	25.19035361236825	26.729166156436058
35	24.92481886276365	24.511435395782122	25.43217069501787	25.131575046436357
36	25.230957226992253	23.37234288012395	25.765120334934704	25.63157955794909
37	25.91077774612558	23.077547747917293	25.0092486011088	26.00242590484833
38	24.87777022994536	22.507679239178493	26.216658309626407	26.39789222124974
39	24.469156076427602	21.582690227934513	27.210737915913136	26.737415779724742
40	24.488491131010463	22.42234719828614	27.531055320169166	25.558106350534228
41	22.97391185535317	21.839588653158767	28.521912417358752	26.664587074129308
42	21.810199370192823	22.116982236240855	29.27623734681803	26.796581046748297
43	20.902998609165074	23.101523215600036	28.801497306626896	27.19398086860799
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	13.0
2	12.0
3	47.0
4	82.0
5	82.0
6	97.0
7	112.0
8	134.0
9	156.0
10	254.0
11	352.0
12	352.0
13	688.5
14	1025.0
15	2078.5
16	3132.0
17	3018.0
18	2904.0
19	2904.0
20	3389.5
21	3875.0
22	3500.5
23	3126.0
24	3568.0
25	4010.0
26	4010.0
27	4557.0
28	5104.0
29	6933.0
30	8762.0
31	9557.0
32	10352.0
33	10352.0
34	11874.5
35	13397.0
36	14783.0
37	16169.0
38	19211.5
39	22254.0
40	22254.0
41	26825.0
42	31396.0
43	34356.5
44	37317.0
45	49480.0
46	61643.0
47	61643.0
48	67499.0
49	73355.0
50	80709.5
51	88064.0
52	88316.5
53	88569.0
54	88569.0
55	79686.0
56	70803.0
57	66444.0
58	62085.0
59	56519.5
60	50954.0
61	50954.0
62	45242.0
63	39530.0
64	31994.0
65	24458.0
66	21080.5
67	17703.0
68	17703.0
69	14846.0
70	11989.0
71	10219.0
72	8449.0
73	6692.0
74	4935.0
75	4935.0
76	4070.0
77	3205.0
78	2694.0
79	2183.0
80	1890.0
81	1597.0
82	1597.0
83	1388.0
84	1179.0
85	933.5
86	688.0
87	580.5
88	473.0
89	473.0
90	371.0
91	269.0
92	174.0
93	79.0
94	53.0
95	27.0
96	27.0
97	15.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	775793.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.57099748318442
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.76968380400109	37.37070677157655
2	6.485486499136621	5.6515823186221645
3	2.174239377337269	2.8420133531340785
4	1.1717116757298969	2.042105858969806
5	0.7070627053199564	1.5403713676974695
6	0.5015457561160573	1.3111709326460734
7	0.3763039859212086	1.1477158016439672
8	0.30289664539371747	1.0558007179291735
9	0.2325522793715019	0.9119281301284058
>10	1.833907437373233	16.339322571815526
>50	0.25100382352494094	7.5573196521883785
>100	0.17249076228761415	14.540213486876674
>500	0.016356882630721492	4.887857633729896
>1k	0.004758365856209888	2.801891403041848
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1827	0.23550096481922367	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1785	0.23008714953602316	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1587	0.20456487748664914	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1579	0.20353367457556334	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1575	0.2030180731200204	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1476	0.19025693709533342	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1475	0.1901280367314477	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1328	0.1711796832402458	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1278	0.16473466504595943	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1177	0.15171572829350097	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1168	0.15055562501852943	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1141	0.1470753151936148	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1114	0.14359500536870015	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1089	0.14037249627155696	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1015	0.13083386934401314	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1009	0.1300604671606988	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	999	0.12877146352184154	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	988	0.12735355951909852	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	987	0.1272246591552128	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	977	0.12593565551635552	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	934	0.12039293986926924	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	934	0.12039293986926924	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	895	0.11536582567772588	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	863	0.11124101403338263	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	842	0.10853410639178233	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	832	0.10724510275292506	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	826	0.10647170056961072	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	821	0.10582719875018207	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	817	0.10531159729463917	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	814	0.10492489620298198	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.8670109165718177E-4	0.0	0.0	0.0	0.0
9	3.8670109165718177E-4	0.0	0.0	0.0	0.0
10	3.8670109165718177E-4	0.0	0.0	0.0	0.0
11	5.15601455542909E-4	0.0	0.0	0.0	0.0
12	6.445018194286363E-4	0.0	0.0	0.0	0.0
13	6.445018194286363E-4	0.0	0.0	0.0	0.0
14	6.445018194286363E-4	0.0	0.0	0.0	0.0
15	6.445018194286363E-4	0.0	0.0	1.2890036388572726E-4	0.0
16	6.445018194286363E-4	0.0	0.0	5.15601455542909E-4	0.0
17	6.445018194286363E-4	0.0	0.0	7.734021833143635E-4	0.0
18	6.445018194286363E-4	0.0	0.0	9.023025472000907E-4	0.0
19	6.445018194286363E-4	0.0	0.0	0.001031202911085818	0.0
20	6.445018194286363E-4	0.0	0.0	0.0019335054582859086	0.0
21	6.445018194286363E-4	0.0	0.0	0.004511512736000454	0.0
22	6.445018194286363E-4	0.0	0.0	0.008378523652572271	0.0
23	6.445018194286363E-4	1.2890036388572726E-4	0.0	0.010569829838629634	0.0
24	6.445018194286363E-4	1.2890036388572726E-4	0.0	0.01405013966354427	0.0
25	6.445018194286363E-4	1.2890036388572726E-4	0.0	0.014694641482972906	0.0
26	6.445018194286363E-4	1.2890036388572726E-4	0.0	0.01830385167177327	0.0
27	6.445018194286363E-4	1.2890036388572726E-4	0.0	0.039056810257375355	0.0
28	6.445018194286363E-4	1.2890036388572726E-4	0.0	0.1220686445997837	0.0
29	6.445018194286363E-4	1.2890036388572726E-4	0.0	0.24516849211065322	0.0
30	6.445018194286363E-4	1.2890036388572726E-4	0.0	0.42279319354518535	0.0
31	6.445018194286363E-4	1.2890036388572726E-4	0.0	0.896502030825233	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGG	25	1.2332559E-4	36.999996	13
GTATCGT	25	1.2332559E-4	36.999996	6
CGTCCGA	25	1.2332559E-4	36.999996	21
AATCGGT	30	3.5984098E-4	30.833332	14
GGTATCA	1305	0.0	29.770115	1
TAGGCCG	25	0.0054952563	29.599998	5
TTAGACT	60	4.3190084E-8	27.749998	4
GCGTACT	45	1.3226707E-4	24.666666	17
CGAACTA	40	0.0019306246	23.125	24
CCGTCCA	40	0.0019306246	23.125	2
GTCCGAC	40	0.0019306246	23.125	22
CGTGCGT	50	2.7010054E-4	22.199999	14
TAGACTC	60	3.724277E-5	21.583332	5
CCGCTTA	70	5.0983963E-6	21.142857	25
GTTAGAC	80	6.954124E-7	20.8125	3
CTGACGT	45	0.003824704	20.555555	4
GTCGTCC	45	0.003824704	20.555555	19
TCGGTCG	45	0.003824704	20.555555	16
TTAGATA	155	0.0	20.290323	4
TACGGCT	110	1.7516868E-9	20.181818	30
>>END_MODULE