FastQCFastQC Report
Fri 10 Feb 2017
ERR1631893.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631893.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130605
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2510.19218253512499522No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2290.17533785077140998No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2270.17380651583017495No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2260.17304084835955746No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2200.16844684353585238No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2100.16079016882967728No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2060.15772749894720722No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1870.14317981700547452No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1670.1278664675931243No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1660.1271008001225068No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1660.1271008001225068No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1660.1271008001225068No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1650.12633513265188928No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1580.1209754603575667No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1490.11408445312200911No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1450.11102178323953907No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1410.10795911335706902No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1380.10566211094521649No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1320.10106810612151142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1350.031.5185181
CTTCTAC303.5821868E-430.8333349
GTATATC303.5821868E-430.8333341
CTACAAA303.5821868E-430.83333412
CCTTCTA303.5821868E-430.8333348
CAGTTGT358.827191E-426.42857227
TATATCC358.827191E-426.4285722
AGTTGTG358.827191E-426.42857228
AAATTCC358.827191E-426.42857216
AACGTCA400.001922085423.12528
TAATACG802.6791895E-823.12527
ACAAATT400.001922085423.12514
CGCGGTA802.6791895E-823.12522
GCGGTAA802.6791895E-823.12523
ATATCCC400.001922085423.1253
CCGTAGT400.001922085423.1251
TCTACAA400.001922085423.12511
CAAATTC400.001922085423.12515
CGGTAAT802.6791895E-823.12524
TCAGTTG400.001922085423.12526
Produced by FastQC (version 0.11.5)