##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631893.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130605
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.630764518969414	34.0	31.0	34.0	31.0	34.0
2	32.79104934726848	34.0	31.0	34.0	31.0	34.0
3	32.8879062823016	34.0	31.0	34.0	31.0	34.0
4	36.28720186822863	37.0	37.0	37.0	35.0	37.0
5	36.21671452088358	37.0	35.0	37.0	35.0	37.0
6	36.269377129512655	37.0	37.0	37.0	35.0	37.0
7	36.26475249799012	37.0	37.0	37.0	35.0	37.0
8	36.23275525439302	37.0	37.0	37.0	35.0	37.0
9	38.078886719497724	39.0	38.0	39.0	37.0	39.0
10	38.00921097967153	39.0	38.0	39.0	35.0	39.0
11	38.08392481145439	39.0	38.0	39.0	37.0	39.0
12	38.02784732590636	39.0	38.0	39.0	35.0	39.0
13	38.08057118793308	39.0	38.0	39.0	37.0	39.0
14	39.484644538876765	41.0	39.0	41.0	37.0	41.0
15	39.49638222120133	41.0	39.0	41.0	37.0	41.0
16	39.44038130240037	41.0	39.0	41.0	37.0	41.0
17	39.41086482140806	40.0	39.0	41.0	37.0	41.0
18	39.42198231308143	41.0	39.0	41.0	37.0	41.0
19	39.444822173729946	41.0	39.0	41.0	37.0	41.0
20	39.43772443627733	41.0	39.0	41.0	37.0	41.0
21	39.399624822939394	40.0	39.0	41.0	37.0	41.0
22	39.385659048275336	40.0	39.0	41.0	37.0	41.0
23	39.31462807702615	40.0	39.0	41.0	36.0	41.0
24	39.330285976800276	41.0	39.0	41.0	36.0	41.0
25	39.26003598637112	40.0	39.0	41.0	36.0	41.0
26	39.21166877225221	40.0	39.0	41.0	36.0	41.0
27	39.09449102254891	40.0	39.0	41.0	36.0	41.0
28	39.09798246621492	40.0	39.0	41.0	36.0	41.0
29	39.082944757091994	40.0	39.0	41.0	35.0	41.0
30	39.0366984418667	40.0	39.0	41.0	35.0	41.0
31	38.94377703763256	40.0	38.0	41.0	35.0	41.0
32	38.904620803185175	40.0	38.0	41.0	35.0	41.0
33	38.85785383407986	40.0	38.0	41.0	35.0	41.0
34	38.82432525554152	40.0	38.0	41.0	35.0	41.0
35	38.74611232341794	40.0	38.0	41.0	35.0	41.0
36	38.70014930515677	40.0	38.0	41.0	35.0	41.0
37	38.65836683128517	40.0	38.0	41.0	35.0	41.0
38	38.615849316641786	40.0	38.0	41.0	35.0	41.0
39	38.547536464913286	40.0	38.0	41.0	35.0	41.0
40	38.22836797978638	40.0	38.0	41.0	34.0	41.0
41	38.302936334749816	40.0	37.0	41.0	34.0	41.0
42	38.295195436621874	40.0	37.0	41.0	34.0	41.0
43	37.82285517399793	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	0.0
18	0.0
19	3.0
20	7.0
21	12.0
22	24.0
23	38.0
24	71.0
25	105.0
26	182.0
27	257.0
28	376.0
29	608.0
30	804.0
31	1186.0
32	1628.0
33	2166.0
34	3089.0
35	4891.0
36	7377.0
37	13467.0
38	30831.0
39	63481.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.84598598828529	16.21377435779641	12.611308908541021	28.328930745377285
2	21.169939895103557	19.58117989357222	32.099077370697906	27.149802840626315
3	21.112514834807243	19.89204088664293	28.74239117951074	30.25305309903909
4	15.754373875425904	15.868458328547911	33.42215075992496	34.955017036101225
5	16.907469086175876	32.97576662455496	33.228436889858735	16.888327399410436
6	34.56529229355691	33.505608514222274	15.518548294475709	16.41055089774511
7	30.942919490065464	27.23708893227671	19.84610083840588	21.973890739251942
8	27.552543930171126	30.592243788522644	20.368286053367022	21.486926227939207
9	28.142107882546608	12.80808544848972	17.466406339726657	41.583400329237016
10	19.16082845220321	23.428658933425215	30.181080356801044	27.229432257570537
11	38.96405191225451	19.377512346387967	18.88442249531029	22.774013246047243
12	22.81765629187244	23.28777611883159	26.827456835496342	27.06711075379962
13	34.93587534933578	17.3109758431913	21.287087018108036	26.46606178936488
14	24.4293863175223	19.922667585467632	22.94322575705371	32.70472033995635
15	29.3089851077677	23.963860495386854	20.023735691589142	26.70341870525631
16	25.9247348876383	23.731863251789747	21.925653688603038	28.417748171968917
17	26.77845411737682	23.766318287967536	21.825351249952146	27.629876344703497
18	26.64905631484246	21.615558363002947	23.234179395888365	28.50120592626622
19	28.537957964855863	21.96546839707515	22.95547643658359	26.541097201485396
20	29.831935990199455	20.67914704643773	22.705103173691665	26.783813789671147
21	27.55713793499483	22.10481987672754	21.825351249952146	28.512690938325484
22	28.48589257685387	22.9470540944068	20.95938134068374	27.60767198805559
23	28.01883541977719	22.158416599670762	21.987672753723057	27.835075226828987
24	27.891734619654684	21.64465372688641	22.527468320508405	27.936143332950497
25	28.190344933195515	22.034378469430727	22.52899965544964	27.246276941924126
26	27.691129742352892	22.896520041346044	22.240343019026838	27.172007197274223
27	27.568622947054095	21.827648252364	23.338310171892346	27.26541862868956
28	26.63221163048888	23.010604494468055	21.691359442594084	28.66582443244899
29	27.082424103211977	23.16450365606217	22.299299414264382	27.45377282646147
30	27.273840970866353	22.369740821561194	23.307683473067648	27.048734734504805
31	27.93767466789173	22.48229393974197	22.378163163737987	27.201868228628307
32	25.62918724397994	22.4179778722101	22.85747100034455	29.09536388346541
33	26.07863404923242	21.97465640672256	24.196623406454577	27.750086137590447
34	26.56100455572145	21.838367596952644	23.394203897247426	28.20642395007848
35	26.162091803529723	23.267868764595537	24.403353623521305	26.166685808353435
36	26.201906512001838	22.29470540944068	24.649898549060143	26.853489529497338
37	27.063282416446537	21.998392098311705	24.021285555683168	26.917039929558594
38	25.901764863519773	21.540522950882433	25.048811301251867	27.508900884345927
39	25.551854829447574	20.835343210443703	25.969143600934114	27.643658359174612
40	25.035029286780752	21.634700049768384	27.105394127330502	26.224876536120362
41	23.563416408253897	20.64622334520118	27.822824547299106	27.967535699245815
42	22.789326595459592	20.825389533325676	28.550208644385744	27.835075226828987
43	22.037441139313195	21.380498449523373	28.465985222617817	28.116075188545615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	2.0
4	3.0
5	3.0
6	6.5
7	10.0
8	10.5
9	11.0
10	13.0
11	15.0
12	15.0
13	30.0
14	45.0
15	100.0
16	155.0
17	148.0
18	141.0
19	141.0
20	171.5
21	202.0
22	195.5
23	189.0
24	221.0
25	253.0
26	253.0
27	313.0
28	373.0
29	511.0
30	649.0
31	822.5
32	996.0
33	996.0
34	1303.0
35	1610.0
36	1824.5
37	2039.0
38	2549.0
39	3059.0
40	3059.0
41	3572.5
42	4086.0
43	4777.5
44	5469.0
45	7411.0
46	9353.0
47	9353.0
48	10247.0
49	11141.0
50	12688.5
51	14236.0
52	14818.0
53	15400.0
54	15400.0
55	14319.0
56	13238.0
57	12605.5
58	11973.0
59	10914.5
60	9856.0
61	9856.0
62	8989.5
63	8123.0
64	6877.0
65	5631.0
66	4847.5
67	4064.0
68	4064.0
69	3563.0
70	3062.0
71	2586.0
72	2110.0
73	1701.0
74	1292.0
75	1292.0
76	1041.5
77	791.0
78	625.0
79	459.0
80	368.0
81	277.0
82	277.0
83	213.5
84	150.0
85	111.5
86	73.0
87	57.5
88	42.0
89	42.0
90	30.5
91	19.0
92	13.5
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	130605.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.66295317943417
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.37360337715131	56.261245740974694
2	5.592504840824083	7.120707476742851
3	1.8810059292578205	3.5925117721373607
4	0.9982320468567717	2.542016002450136
5	0.6602763779811659	2.101757206845067
6	0.4305627382828003	1.6446537268864132
7	0.28263196507390526	1.2595229891658053
8	0.25376742395997454	1.2924466904023582
9	0.20205178779751526	1.1576892155736764
>10	1.170216604327276	14.073733777420466
>50	0.1130527860295621	4.896443474598982
>100	0.04209412245781568	4.05727192680219
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	251	0.19218253512499522	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	229	0.17533785077140998	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	227	0.17380651583017495	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	226	0.17304084835955746	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	220	0.16844684353585238	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	210	0.16079016882967728	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	206	0.15772749894720722	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	187	0.14317981700547452	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	167	0.1278664675931243	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	166	0.1271008001225068	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	166	0.1271008001225068	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	166	0.1271008001225068	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	165	0.12633513265188928	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	158	0.1209754603575667	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	149	0.11408445312200911	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	145	0.11102178323953907	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	141	0.10795911335706902	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	138	0.10566211094521649	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	132	0.10106810612151142	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	7.656674706175108E-4	0.0
11	0.0	0.0	0.0	7.656674706175108E-4	0.0
12	0.0	0.0	0.0	7.656674706175108E-4	0.0
13	0.0	0.0	0.0	7.656674706175108E-4	0.0
14	0.0	0.0	0.0	7.656674706175108E-4	0.0
15	0.0	0.0	0.0	7.656674706175108E-4	0.0
16	0.0	0.0	0.0	7.656674706175108E-4	0.0
17	0.0	0.0	0.0	0.0015313349412350216	0.0
18	7.656674706175108E-4	0.0	0.0	0.0015313349412350216	0.0
19	7.656674706175108E-4	0.0	0.0	0.0022970024118525324	0.0
20	7.656674706175108E-4	0.0	0.0	0.0030626698824700433	0.0
21	7.656674706175108E-4	0.0	0.0	0.0038283373530875542	0.0
22	7.656674706175108E-4	0.0	0.0	0.0038283373530875542	0.0
23	7.656674706175108E-4	0.0	0.0	0.004594004823705065	0.0
24	7.656674706175108E-4	0.0	0.0	0.006891007235557597	0.0
25	7.656674706175108E-4	0.0	0.0	0.00842234217679262	0.0
26	7.656674706175108E-4	0.0	0.0	0.010719344588645152	0.0
27	7.656674706175108E-4	0.0	0.0	0.025267026530377858	0.0
28	7.656674706175108E-4	0.0	0.0	0.08881742659163125	0.0
29	7.656674706175108E-4	0.0	0.0	0.20902721947858044	0.0
30	7.656674706175108E-4	0.0	0.0	0.36598905095517015	0.0
31	7.656674706175108E-4	0.0	0.0	0.7947628345009762	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	135	0.0	31.518518	1
CTTCTAC	30	3.5821868E-4	30.833334	9
GTATATC	30	3.5821868E-4	30.833334	1
CTACAAA	30	3.5821868E-4	30.833334	12
CCTTCTA	30	3.5821868E-4	30.833334	8
CAGTTGT	35	8.827191E-4	26.428572	27
TATATCC	35	8.827191E-4	26.428572	2
AGTTGTG	35	8.827191E-4	26.428572	28
AAATTCC	35	8.827191E-4	26.428572	16
AACGTCA	40	0.0019220854	23.125	28
TAATACG	80	2.6791895E-8	23.125	27
ACAAATT	40	0.0019220854	23.125	14
CGCGGTA	80	2.6791895E-8	23.125	22
GCGGTAA	80	2.6791895E-8	23.125	23
ATATCCC	40	0.0019220854	23.125	3
CCGTAGT	40	0.0019220854	23.125	1
TCTACAA	40	0.0019220854	23.125	11
CAAATTC	40	0.0019220854	23.125	15
CGGTAAT	80	2.6791895E-8	23.125	24
TCAGTTG	40	0.0019220854	23.125	26
>>END_MODULE