FastQCFastQC Report
Fri 10 Feb 2017
ERR1631892.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631892.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734131
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15960.21739989184491595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15500.21113398017520035No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG13800.18797735009146868No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG13690.18647897990958015No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC13680.1863427644384994No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC13550.18457196331444933No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA12820.1746282339255528No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12710.1731298637436643No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT12600.17163149356177576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12380.1686347531979987No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG11190.15242511213938656No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA10920.14874729442020565No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC10780.1468402778250748No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC10750.1464316314118325No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA10500.14302624463481312No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10470.1426175982215708No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA10240.139484642386713No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG10180.13866734956022836No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG10040.13676033296509751No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT9780.13321873071699739No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC9730.13253765336159348No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9660.13158414506402807No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9600.13076685223754342No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG9390.12790632734484716No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG9350.12736146546052407No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA9120.12422850962566627No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9090.12381986321242394No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT8740.11905232172459684No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8730.11891610625351606No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC8670.11809881342703141No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8510.11591936588973902No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG8470.11537450400541593No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA8460.11523828853433515No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8310.11319505646812354No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8240.1122415481705581No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8160.11115182440191193No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG8020.10924480780678109No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC7840.10679292932732713No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC7830.10665671385624637No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG7830.10665671385624637No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG7820.10652049838516559No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7770.10583942102976172No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA7680.10461348179003475No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7620.10379618896355011No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7520.10243403425274236No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7500.10216160331058081No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC7420.10107187954193461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10000.030.8949991
GCTAAAC358.866328E-426.4285723
TATACTA509.0852445E-625.9000025
CTACGGG451.3225761E-424.66666611
TAAACCA551.9014451E-523.5454545
AAACACG400.001930526523.12515
GTTTAGA502.700813E-422.21
ATAATAC502.700813E-422.23
CTAAACC753.73695E-722.24
TATAATA502.700813E-422.22
CTTATAC8350.022.1556937
GCGGTAA3700.021.023
ATACACA1251.8189894E-1120.7237
ATACTAC450.003824511520.5555556
GTAACAC450.003824511520.5555553
CGCGGTA3800.020.44736722
TTCTGCG3050.020.01639418
AACGATT656.89779E-519.92307722
ACTGTCC656.89779E-519.9230778
GTATCAA15550.019.8681662
Produced by FastQC (version 0.11.5)