##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631892.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	734131
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61564897817964	34.0	31.0	34.0	31.0	34.0
2	32.78355497860736	34.0	31.0	34.0	31.0	34.0
3	32.88230166005795	34.0	31.0	34.0	31.0	34.0
4	36.28064745937714	37.0	37.0	37.0	35.0	37.0
5	36.215359384088124	37.0	35.0	37.0	35.0	37.0
6	36.27160956287093	37.0	37.0	37.0	35.0	37.0
7	36.25118405298237	37.0	37.0	37.0	35.0	37.0
8	36.23877618572162	37.0	37.0	37.0	35.0	37.0
9	38.08043932213733	39.0	38.0	39.0	37.0	39.0
10	38.019421601866696	39.0	38.0	39.0	35.0	39.0
11	38.09407721510194	39.0	38.0	39.0	37.0	39.0
12	38.010769195143645	39.0	38.0	39.0	35.0	39.0
13	38.074218361573074	39.0	38.0	39.0	37.0	39.0
14	39.47188444569157	41.0	39.0	41.0	37.0	41.0
15	39.46759365835253	41.0	39.0	41.0	37.0	41.0
16	39.437051425426795	40.0	39.0	41.0	37.0	41.0
17	39.38188960825793	40.0	39.0	41.0	37.0	41.0
18	39.406577300236606	41.0	39.0	41.0	37.0	41.0
19	39.411798439243135	41.0	39.0	41.0	37.0	41.0
20	39.42307299378449	41.0	39.0	41.0	37.0	41.0
21	39.38615042819333	40.0	39.0	41.0	37.0	41.0
22	39.36487765807465	40.0	39.0	41.0	36.0	41.0
23	39.298854019241794	40.0	39.0	41.0	36.0	41.0
24	39.31798275784567	41.0	39.0	41.0	36.0	41.0
25	39.258936075441575	40.0	39.0	41.0	36.0	41.0
26	39.19346955788544	40.0	39.0	41.0	36.0	41.0
27	39.08617671777925	40.0	39.0	41.0	36.0	41.0
28	39.07959342406192	40.0	39.0	41.0	35.0	41.0
29	39.0387424042848	40.0	39.0	41.0	35.0	41.0
30	38.992015049085246	40.0	38.0	41.0	35.0	41.0
31	38.86591766319635	40.0	38.0	41.0	35.0	41.0
32	38.822443133446214	40.0	38.0	41.0	35.0	41.0
33	38.74976264454164	40.0	38.0	41.0	35.0	41.0
34	38.689763815994695	40.0	38.0	41.0	35.0	41.0
35	38.62335877384282	40.0	38.0	41.0	35.0	41.0
36	38.54947686448332	40.0	38.0	41.0	35.0	41.0
37	38.48262231127687	40.0	38.0	41.0	35.0	41.0
38	38.458821382014925	40.0	38.0	41.0	35.0	41.0
39	38.3679724735776	40.0	38.0	41.0	34.0	41.0
40	38.010596201495375	40.0	37.0	41.0	34.0	41.0
41	38.064931190754784	40.0	37.0	41.0	34.0	41.0
42	38.02931220722187	40.0	37.0	41.0	34.0	41.0
43	37.54885844624461	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	0.0
18	3.0
19	5.0
20	23.0
21	36.0
22	92.0
23	183.0
24	327.0
25	575.0
26	970.0
27	1556.0
28	2378.0
29	3588.0
30	5118.0
31	7120.0
32	9811.0
33	13148.0
34	18396.0
35	27798.0
36	42950.0
37	82472.0
38	171914.0
39	345664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.6532022214019	16.461503464640508	12.012297532729173	26.87299678122842
2	20.35603999831093	19.383870181207442	33.44280516692525	26.817284653556385
3	21.83929026290948	19.11797758165777	28.175489115702785	30.86724303972997
4	14.739467479237355	16.066614813977342	33.21600640757576	35.977911299209545
5	17.845588866292257	32.108438412217986	32.349676011502034	17.696296709987728
6	35.58928856021609	32.44802358162235	15.71953779366353	16.243150064498025
7	31.934355040176754	27.241323415030834	19.159931946750646	21.664389598041765
8	27.732516403748104	30.696156408052516	20.013866734956025	21.55746045324336
9	28.07114806485491	12.517384499496684	18.002236658035145	41.40923077761326
10	19.057906558911146	23.75270898518112	30.170909551565046	27.01847490434269
11	38.96579765736633	19.76050595874578	18.545191525763112	22.728504858124776
12	22.92955889344	23.151590111301662	26.58135945764448	27.33749153761386
13	34.733310539944505	17.375645491063587	21.698171034869798	26.192872934122114
14	24.674887724397962	19.72291048872749	23.602463320579027	31.999738466295526
15	28.25245085686342	24.437736589246335	21.220735808731682	26.08907674515856
16	26.902691753924028	23.21751839930476	22.64990853131117	27.229881315460048
17	26.500175036880343	23.387651522684642	22.263465239855012	27.848708200580006
18	26.374856803486026	20.969009618174415	24.200313023152546	28.455820555187017
19	29.239468160314715	22.817862207153762	23.129386989515496	24.81328264301603
20	29.979935461109804	21.184502493424198	22.820995162988623	26.01456688247738
21	26.92230678175966	22.803832013632444	22.376660296323138	27.897200908284763
22	28.670223706668157	22.201078554100018	22.074261950523816	27.05443578870801
23	26.186607022452396	22.38987319701797	23.250891189719546	28.17262859081009
24	27.12227109330624	22.24902639992045	23.123802155201183	27.504900351572132
25	27.36405355447461	22.28253540580632	23.796025504984804	26.557385534734262
26	27.96503621288299	22.661895492766277	22.139917807584748	27.233150486765982
27	27.71821377928462	21.942269159046546	23.22160486343718	27.117912198231654
28	26.503444208186277	23.228143206049058	22.67115814479977	27.597254440964896
29	27.56102112565741	23.566502436213703	23.050245800817564	25.82223063731132
30	27.50381062780348	21.667386338405542	23.05950845285106	27.769294580939913
31	26.465712522696904	21.94676426959221	23.383292627610057	28.204230580100827
32	25.2265603822751	22.698128808073765	23.33602585914503	28.73928495050611
33	26.14696832036789	21.361991252242447	24.978512009437008	27.51252841795265
34	26.763343327008393	21.943222667344113	24.48622929695109	26.807204708696403
35	26.801075012497773	22.530583778644413	23.7138875759231	26.954453632934722
36	25.561378010191643	21.83547622971922	24.27877313449507	28.32437262559407
37	25.41848798102791	21.727729792094326	26.018925777551967	26.8348564493258
38	25.05996886114331	20.804052682695595	25.053702949473593	29.0822755066875
39	24.469066147594912	20.91956340217209	27.678030215315797	26.933340234917203
40	25.855330996783955	20.909074810898872	26.685564293021276	26.5500298992959
41	23.30605845550726	21.13342169176891	27.947736848055726	27.612783004668106
42	21.894457528697195	22.130655155551256	28.347256824735638	27.627630491015907
43	22.168795487453874	21.537300563523402	27.573280518054677	28.72062343096804
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8.0
1	14.0
2	20.0
3	54.0
4	88.0
5	88.0
6	114.0
7	140.0
8	160.5
9	181.0
10	273.0
11	365.0
12	365.0
13	716.5
14	1068.0
15	1987.0
16	2906.0
17	2811.0
18	2716.0
19	2716.0
20	3099.0
21	3482.0
22	2943.0
23	2404.0
24	2502.5
25	2601.0
26	2601.0
27	2745.0
28	2889.0
29	3407.0
30	3925.0
31	4761.0
32	5597.0
33	5597.0
34	8948.0
35	12299.0
36	13024.0
37	13749.0
38	15341.5
39	16934.0
40	16934.0
41	19479.5
42	22025.0
43	27608.0
44	33191.0
45	41497.0
46	49803.0
47	49803.0
48	54385.0
49	58967.0
50	65845.5
51	72724.0
52	76626.5
53	80529.0
54	80529.0
55	75287.5
56	70046.0
57	62977.5
58	55909.0
59	55521.5
60	55134.0
61	55134.0
62	52041.0
63	48948.0
64	38319.5
65	27691.0
66	25995.5
67	24300.0
68	24300.0
69	22344.0
70	20388.0
71	20914.0
72	21440.0
73	17698.5
74	13957.0
75	13957.0
76	8637.0
77	3317.0
78	2614.5
79	1912.0
80	1537.0
81	1162.0
82	1162.0
83	907.5
84	653.0
85	502.5
86	352.0
87	269.5
88	187.0
89	187.0
90	142.0
91	97.0
92	60.5
93	24.0
94	13.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	734131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.8701659331228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.48627623335877	36.21194736828626
2	6.4472250551101435	5.39892765731297
3	2.013472505653895	2.5291328374052737
4	1.0642153263427354	1.7823548921017105
5	0.6727495629502012	1.4084067916080383
6	0.4742923987542661	1.191522086199598
7	0.32452451006855415	0.951152656015502
8	0.2458835164848679	0.8236146908352923
9	0.20176747614026358	0.7603233935310209
>10	1.6107186505019147	13.366131149615605
>50	0.2191494438165324	6.511279263615882
>100	0.2096780054019971	18.8801798862741
>500	0.023841891579977854	7.01574438768266
>1k	0.006205423835884647	3.1692829395160866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1596	0.21739989184491595	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1550	0.21113398017520035	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	1380	0.18797735009146868	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1369	0.18647897990958015	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	1368	0.1863427644384994	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1355	0.18457196331444933	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	1282	0.1746282339255528	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1271	0.1731298637436643	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	1260	0.17163149356177576	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1238	0.1686347531979987	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	1119	0.15242511213938656	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	1092	0.14874729442020565	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	1078	0.1468402778250748	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	1075	0.1464316314118325	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	1050	0.14302624463481312	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1047	0.1426175982215708	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	1024	0.139484642386713	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	1018	0.13866734956022836	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	1004	0.13676033296509751	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	978	0.13321873071699739	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	973	0.13253765336159348	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	966	0.13158414506402807	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	960	0.13076685223754342	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	939	0.12790632734484716	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	935	0.12736146546052407	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	912	0.12422850962566627	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	909	0.12381986321242394	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	874	0.11905232172459684	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	873	0.11891610625351606	No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC	867	0.11809881342703141	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	851	0.11591936588973902	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	847	0.11537450400541593	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	846	0.11523828853433515	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	831	0.11319505646812354	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	824	0.1122415481705581	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	816	0.11115182440191193	No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG	802	0.10924480780678109	No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC	784	0.10679292932732713	No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC	783	0.10665671385624637	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	783	0.10665671385624637	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	782	0.10652049838516559	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	777	0.10583942102976172	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	768	0.10461348179003475	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	762	0.10379618896355011	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	752	0.10243403425274236	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	750	0.10216160331058081	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	742	0.10107187954193461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.362154710807744E-4
7	0.0	0.0	0.0	0.0	1.362154710807744E-4
8	0.0	0.0	0.0	0.0	1.362154710807744E-4
9	0.0	0.0	0.0	0.0	1.362154710807744E-4
10	0.0	0.0	0.0	0.0	1.362154710807744E-4
11	0.0	0.0	0.0	0.0	1.362154710807744E-4
12	0.0	0.0	0.0	0.0	1.362154710807744E-4
13	0.0	0.0	0.0	1.362154710807744E-4	1.362154710807744E-4
14	0.0	0.0	0.0	2.724309421615488E-4	1.362154710807744E-4
15	1.362154710807744E-4	0.0	0.0	4.0864641324232326E-4	1.362154710807744E-4
16	1.362154710807744E-4	0.0	0.0	9.535082975654209E-4	1.362154710807744E-4
17	1.362154710807744E-4	0.0	0.0	0.0012259392397269697	1.362154710807744E-4
18	2.724309421615488E-4	0.0	0.0	0.0013621547108077442	1.362154710807744E-4
19	2.724309421615488E-4	0.0	0.0	0.0017708011240500673	1.362154710807744E-4
20	2.724309421615488E-4	0.0	0.0	0.0024518784794539394	1.362154710807744E-4
21	2.724309421615488E-4	0.0	0.0	0.004495110545665555	1.362154710807744E-4
22	2.724309421615488E-4	0.0	0.0	0.006402127140796398	1.362154710807744E-4
23	2.724309421615488E-4	0.0	0.0	0.007355635438361818	1.362154710807744E-4
24	2.724309421615488E-4	0.0	0.0	0.009398867504573435	1.362154710807744E-4
25	2.724309421615488E-4	0.0	0.0	0.011305884099704277	1.362154710807744E-4
26	2.724309421615488E-4	0.0	0.0	0.01389397805023899	1.362154710807744E-4
27	4.0864641324232326E-4	0.0	0.0	0.03487116059667825	1.362154710807744E-4
28	5.448618843230976E-4	0.0	0.0	0.14997323365993262	1.362154710807744E-4
29	5.448618843230976E-4	0.0	0.0	0.3173820476182044	1.362154710807744E-4
30	5.448618843230976E-4	0.0	0.0	0.5188447293466697	1.362154710807744E-4
31	5.448618843230976E-4	0.0	0.0	1.1849383829316567	1.362154710807744E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1000	0.0	30.894999	1
GCTAAAC	35	8.866328E-4	26.428572	3
TATACTA	50	9.0852445E-6	25.900002	5
CTACGGG	45	1.3225761E-4	24.666666	11
TAAACCA	55	1.9014451E-5	23.545454	5
AAACACG	40	0.0019305265	23.125	15
GTTTAGA	50	2.700813E-4	22.2	1
ATAATAC	50	2.700813E-4	22.2	3
CTAAACC	75	3.73695E-7	22.2	4
TATAATA	50	2.700813E-4	22.2	2
CTTATAC	835	0.0	22.15569	37
GCGGTAA	370	0.0	21.0	23
ATACACA	125	1.8189894E-11	20.72	37
ATACTAC	45	0.0038245115	20.555555	6
GTAACAC	45	0.0038245115	20.555555	3
CGCGGTA	380	0.0	20.447367	22
TTCTGCG	305	0.0	20.016394	18
AACGATT	65	6.89779E-5	19.923077	22
ACTGTCC	65	6.89779E-5	19.923077	8
GTATCAA	1555	0.0	19.868166	2
>>END_MODULE