FastQCFastQC Report
Fri 10 Feb 2017
ERR1631891.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631891.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421800
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9910.23494547178757705No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8560.2029397818871503No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7690.18231389284020863No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7510.17804646752015174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.1498340445708867No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5970.1415362731152205No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5960.14129919393077287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5960.14129919393077287No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5940.14082503556187767No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5650.1339497392128971No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5630.13347558084400188No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5520.13086770981507823No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4980.11806543385490754No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4920.11664295874822189No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4920.11664295874822189No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4910.1164058795637743No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4730.11213845424371739No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4240.10052157420578473No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4220.10004741583688952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAAC200.001840615837.01
AAATCGT303.5956496E-430.8333345
AATCGTA303.5956496E-430.8333346
GGTATCA6300.030.5396841
TAGGACA454.0003524E-628.7777794
ATCGTAC358.860051E-426.428577
TCGTACC358.860051E-426.428578
TATGAGA509.073288E-625.8999984
ATACGGC509.073288E-625.89999829
TGCGGGT2500.025.16000221
GTAACGT2450.024.91836726
TTTTACG451.3212727E-424.6666684
CTGCGGG2550.024.66666620
TTCTGCG2650.023.7358518
TACGGCT551.8989593E-523.54545430
AACGACC551.8989593E-523.54545437
GGTAACG2600.023.4807725
TAGAACA802.7153874E-823.1254
CGTTAGA400.001929172223.1251
AATGAGC2000.023.12499834
Produced by FastQC (version 0.11.5)