FastQCFastQC Report
Fri 10 Feb 2017
ERR1631890.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631890.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219831
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7010.3188813224704432No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6550.29795615722987207No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC6370.28976804909225723No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5380.2447334543353758No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5340.24291387474923917No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5150.23427087171509023No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4910.22335339419827047No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4640.2110712319918483No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4630.21061633709531413No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT4600.20925165240571167No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4250.19333033102701622No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG3310.15057021075280558No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3280.1492055260632031No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3140.1428369975117249No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3040.13828804854638335No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT2810.12782546592609778No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2740.12464120165035868No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2680.12191183227115375No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2600.11827267309888051No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2570.11690798840927803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.11463351392660724No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT2490.11326882923700478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2430.11053945985779985No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2420.11008456496126569No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG2400.10917477516819739No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG2370.10781009047859492No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2370.10781009047859492No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT2350.10690030068552661No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC2300.10462582620285583No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2300.10462582620285583No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC2250.10235135172018506No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2230.10144156192711674No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2220.1009866670305826No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2210.10053177213404843No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA2210.10053177213404843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTAG200.001838699837.01
TCCAGAG200.001838699837.02
TTCGGTT200.001838699837.031
CAACGAT250.00548679729.59999821
TTTCGGT250.00548679729.59999830
CTAACTG250.00548679729.59999836
AACGATT250.00548679729.59999822
TGTAGTC250.00548679729.5999983
GGTATCA3500.028.0142861
TAAAGTC358.846497E-426.4285728
TAAAAAT358.846497E-426.4285724
TAACCAG358.846497E-426.428575
ATACACA358.846497E-426.4285737
CCGCTTA400.001926249123.12525
AAAAATT400.001926249123.12525
ACCAGGA400.001926249123.1252
CTTATAC2200.022.70454637
TAAAGAC1005.275069E-1022.19999937
TCTTATA5300.021.6415137
CAGTCGG957.064955E-921.42105310
Produced by FastQC (version 0.11.5)