##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631890.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219831
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.605783533714536	34.0	31.0	34.0	31.0	34.0
2	32.79090756080807	34.0	31.0	34.0	31.0	34.0
3	32.865542166482435	34.0	31.0	34.0	31.0	34.0
4	36.2948810677293	37.0	37.0	37.0	35.0	37.0
5	36.209228907660886	37.0	35.0	37.0	35.0	37.0
6	36.26574504960629	37.0	37.0	37.0	35.0	37.0
7	36.25068802853101	37.0	37.0	37.0	35.0	37.0
8	36.22979015698423	37.0	37.0	37.0	35.0	37.0
9	38.072018960019285	39.0	38.0	39.0	37.0	39.0
10	38.00801524807693	39.0	38.0	39.0	35.0	39.0
11	38.08330945135126	39.0	38.0	39.0	37.0	39.0
12	38.025778893786594	39.0	38.0	39.0	35.0	39.0
13	38.066473791230536	39.0	38.0	39.0	35.0	39.0
14	39.46431122089241	40.0	39.0	41.0	37.0	41.0
15	39.46519371699169	41.0	39.0	41.0	37.0	41.0
16	39.415628369065324	40.0	39.0	41.0	37.0	41.0
17	39.36108647097088	40.0	39.0	41.0	36.0	41.0
18	39.383667453634835	40.0	39.0	41.0	36.0	41.0
19	39.38444532390791	40.0	39.0	41.0	36.0	41.0
20	39.391086789397306	40.0	39.0	41.0	36.0	41.0
21	39.35988554844403	40.0	39.0	41.0	36.0	41.0
22	39.32705578376116	40.0	39.0	41.0	36.0	41.0
23	39.274761066455596	40.0	39.0	41.0	36.0	41.0
24	39.283317639459405	40.0	39.0	41.0	36.0	41.0
25	39.2309228452766	40.0	39.0	41.0	36.0	41.0
26	39.17002606547757	40.0	39.0	41.0	36.0	41.0
27	39.07622673781223	40.0	39.0	41.0	35.0	41.0
28	39.077373072951495	40.0	39.0	41.0	35.0	41.0
29	39.057585144952256	40.0	39.0	41.0	35.0	41.0
30	39.01041254418167	40.0	39.0	41.0	35.0	41.0
31	38.94654075175931	40.0	38.0	41.0	35.0	41.0
32	38.916458552251505	40.0	38.0	41.0	35.0	41.0
33	38.847214451101074	40.0	38.0	41.0	35.0	41.0
34	38.813875204134085	40.0	38.0	41.0	35.0	41.0
35	38.73619735160191	40.0	38.0	41.0	35.0	41.0
36	38.71796061519986	40.0	38.0	41.0	35.0	41.0
37	38.66615263543358	40.0	38.0	41.0	35.0	41.0
38	38.647119832962595	40.0	38.0	41.0	35.0	41.0
39	38.58956198170413	40.0	38.0	41.0	35.0	41.0
40	38.25885794087276	40.0	38.0	41.0	34.0	41.0
41	38.33707711833181	40.0	38.0	41.0	34.0	41.0
42	38.33828713875659	40.0	37.0	41.0	34.0	41.0
43	37.880480914884615	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	1.0
20	7.0
21	16.0
22	26.0
23	38.0
24	87.0
25	160.0
26	265.0
27	433.0
28	638.0
29	942.0
30	1443.0
31	1875.0
32	2812.0
33	3819.0
34	5488.0
35	8901.0
36	13152.0
37	22388.0
38	50020.0
39	107319.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.687337090765176	15.521013869745396	12.645168333856462	29.146480705632964
2	20.605374128307655	17.982905049788247	33.05402786686136	28.357692955042737
3	21.21038434069808	18.878593101064	29.070513257911763	30.840509300326158
4	15.884019997179651	15.430944680231631	33.08450582492915	35.600529497659565
5	17.286915858090985	32.153335971723735	33.28465957940418	17.275088590781102
6	34.97050006595976	32.41171627295513	15.616541798017568	17.001241863067538
7	31.117085397418926	27.05350928667931	19.8948282999213	21.93457701598046
8	28.240330071736924	30.147249478008103	20.132738330808667	21.479682119446302
9	29.461267974034598	12.455022267105186	17.126337959614432	40.957371799245784
10	19.996269861848422	23.112299903107385	28.570128871724187	28.321301363320007
11	39.31019737889561	18.95228607430253	18.05887249750945	23.678644049292412
12	24.27000741478681	22.840727649876495	25.554175707702736	27.335089227633958
13	34.72485682183132	16.85658528596968	20.719552747337726	27.699005144861278
14	24.250446934235846	20.138197069567077	23.051798881868343	32.55955711432873
15	30.57348599606061	22.931251734286793	20.02037929136473	26.474882978287866
16	25.627413786044734	23.692290896188435	21.39416187889788	29.28613343886895
17	27.01575301026698	23.99707047686632	21.32410806483162	27.663068448035084
18	27.156315533296034	20.447980494106837	22.783865787809727	29.611838184787402
19	28.29582724911409	21.601593951717454	22.542771492646622	27.559807306521826
20	29.842015002433687	20.230085838666977	22.5837120333347	27.34418712556464
21	29.050497882464256	21.271795151730196	21.190823860147113	28.48688310565844
22	30.125414522974463	22.329425786172106	20.38384031369552	27.161319377157906
23	28.983173437777204	21.796743862330608	21.168988905113473	28.051093794778716
24	28.943142686882194	20.99794842401663	22.248454494589026	27.810454394512146
25	27.866861361682382	20.961556832293898	23.197820143655807	27.973761662367906
26	28.635633736825106	22.23571743748607	22.374005486032452	26.75464333965637
27	28.14207277408555	20.982027102637936	23.511242727367843	27.364657395908676
28	26.5485759515264	21.730329207436622	21.96869413322052	29.752400707816456
29	28.007423884711436	22.235262542589535	22.49773689788974	27.25957667480928
30	27.192707125018767	20.59491154568737	23.680918523775084	28.531462805518785
31	27.83274424444232	21.81539455308851	22.723364766570683	27.628496435898487
32	26.09095168561304	21.714862780954462	22.478631312235308	29.715554221197195
33	26.753733549863306	21.239952508972802	24.272736784166018	27.733577156997875
34	27.05987781523079	21.548826143719495	23.644526932052347	27.746769108997366
35	26.38754315815331	23.082276839936135	24.711255464424944	25.818924537485614
36	26.781937033448422	21.556559356960577	24.03983059714053	27.621673012450472
37	25.673358170594685	22.090151070595137	24.70852609504574	27.527964663764436
38	26.44258544063394	20.633122716996237	25.162511201786824	27.761780640582995
39	25.17388357420018	19.75335598709918	26.667758414418348	28.40500202428229
40	25.097916126478975	20.694078633131817	27.81090928940868	26.397095950980525
41	22.804790953050297	19.732885716755145	28.915394098193616	28.546929232000945
42	21.41827130841419	20.649044038374935	29.50311830451574	28.429566348695133
43	21.007956111740384	20.181412084737822	29.7210129599556	29.089618843566196
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	3.0
2	1.0
3	3.0
4	5.0
5	5.0
6	8.0
7	11.0
8	13.5
9	16.0
10	27.5
11	39.0
12	39.0
13	84.5
14	130.0
15	267.5
16	405.0
17	390.0
18	375.0
19	375.0
20	417.0
21	459.0
22	359.0
23	259.0
24	278.5
25	298.0
26	298.0
27	317.0
28	336.0
29	453.0
30	570.0
31	765.0
32	960.0
33	960.0
34	1369.5
35	1779.0
36	2162.0
37	2545.0
38	3318.5
39	4092.0
40	4092.0
41	4882.5
42	5673.0
43	7189.5
44	8706.0
45	12582.0
46	16458.0
47	16458.0
48	18263.0
49	20068.0
50	22866.5
51	25665.0
52	26157.5
53	26650.0
54	26650.0
55	24503.0
56	22356.0
57	20604.5
58	18853.0
59	17249.5
60	15646.0
61	15646.0
62	14861.0
63	14076.0
64	11205.5
65	8335.0
66	7407.5
67	6480.0
68	6480.0
69	5546.0
70	4612.0
71	4368.5
72	4125.0
73	4473.0
74	4821.0
75	4821.0
76	4060.0
77	3299.0
78	2190.5
79	1082.0
80	684.5
81	287.0
82	287.0
83	225.5
84	164.0
85	133.0
86	102.0
87	79.0
88	56.0
89	56.0
90	39.5
91	23.0
92	15.5
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	219831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.78838244338178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.66399052251954	38.30838794784593
2	7.0879991970694505	6.490960359875979
3	2.578010936087141	3.5412885205433597
4	1.3909999413095386	2.547665491656111
5	0.8689999025674491	1.989504994100993
6	0.6719999238883747	1.8461873710154608
7	0.4999999433600196	1.6025932039766464
8	0.3579999594456653	1.3113791246250632
9	0.3019999657893587	1.2445280938306231
>10	2.1629997549747775	19.987542439990563
>50	0.26699996975416806	8.36141557080243
>100	0.14199998391420174	11.129322975955528
>500	0.005999999320318384	1.639223905781321
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	701	0.3188813224704432	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	655	0.29795615722987207	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	637	0.28976804909225723	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	538	0.2447334543353758	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	534	0.24291387474923917	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	515	0.23427087171509023	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	491	0.22335339419827047	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	464	0.2110712319918483	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	463	0.21061633709531413	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	460	0.20925165240571167	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	425	0.19333033102701622	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	331	0.15057021075280558	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	328	0.1492055260632031	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	314	0.1428369975117249	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	304	0.13828804854638335	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	281	0.12782546592609778	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	274	0.12464120165035868	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	268	0.12191183227115375	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	260	0.11827267309888051	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	257	0.11690798840927803	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	252	0.11463351392660724	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	249	0.11326882923700478	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	243	0.11053945985779985	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	242	0.11008456496126569	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	240	0.10917477516819739	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG	237	0.10781009047859492	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	237	0.10781009047859492	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	235	0.10690030068552661	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	230	0.10462582620285583	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	230	0.10462582620285583	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	225	0.10235135172018506	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	223	0.10144156192711674	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	222	0.1009866670305826	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	221	0.10053177213404843	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	221	0.10053177213404843	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.548948965341558E-4	0.0	0.0	0.0	0.0
9	4.548948965341558E-4	0.0	0.0	0.0	0.0
10	4.548948965341558E-4	0.0	0.0	0.0	0.0
11	4.548948965341558E-4	0.0	0.0	0.0	0.0
12	9.097897930683116E-4	0.0	0.0	0.0	0.0
13	9.097897930683116E-4	0.0	0.0	0.0	0.0
14	9.097897930683116E-4	0.0	0.0	0.0	0.0
15	9.097897930683116E-4	0.0	0.0	4.548948965341558E-4	0.0
16	9.097897930683116E-4	0.0	0.0	0.0013646846896024673	0.0
17	0.0013646846896024673	0.0	0.0	0.0018195795861366232	0.0
18	0.0013646846896024673	0.0	0.0	0.0018195795861366232	0.0
19	0.0013646846896024673	0.0	0.0	0.0018195795861366232	0.0
20	0.0013646846896024673	0.0	0.0	0.0036391591722732463	0.0
21	0.0018195795861366232	0.0	0.0	0.005003843861875714	0.0
22	0.0018195795861366232	0.0	0.0	0.009097897930683116	0.0
23	0.0018195795861366232	0.0	0.0	0.009552792827217271	0.0
24	0.0018195795861366232	0.0	0.0	0.01182726730988805	0.0
25	0.0018195795861366232	0.0	0.0	0.013646846896024674	0.0
26	0.0018195795861366232	0.0	0.0	0.0195604805509687	0.0
27	0.0018195795861366232	0.0	0.0	0.04094054068807402	0.0
28	0.0018195795861366232	0.0	0.0	0.13555867916717843	0.0
29	0.0018195795861366232	0.0	0.0	0.30750895005708934	0.0
30	0.0018195795861366232	0.0	0.0	0.534501503427633	0.0
31	0.0018195795861366232	0.0	0.0	1.238223908365972	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTGTAG	20	0.0018386998	37.0	1
TCCAGAG	20	0.0018386998	37.0	2
TTCGGTT	20	0.0018386998	37.0	31
CAACGAT	25	0.005486797	29.599998	21
TTTCGGT	25	0.005486797	29.599998	30
CTAACTG	25	0.005486797	29.599998	36
AACGATT	25	0.005486797	29.599998	22
TGTAGTC	25	0.005486797	29.599998	3
GGTATCA	350	0.0	28.014286	1
TAAAGTC	35	8.846497E-4	26.42857	28
TAAAAAT	35	8.846497E-4	26.42857	24
TAACCAG	35	8.846497E-4	26.42857	5
ATACACA	35	8.846497E-4	26.42857	37
CCGCTTA	40	0.0019262491	23.125	25
AAAAATT	40	0.0019262491	23.125	25
ACCAGGA	40	0.0019262491	23.125	2
CTTATAC	220	0.0	22.704546	37
TAAAGAC	100	5.275069E-10	22.199999	37
TCTTATA	530	0.0	21.64151	37
CAGTCGG	95	7.064955E-9	21.421053	10
>>END_MODULE