FastQCFastQC Report
Fri 10 Feb 2017
ERR1631889.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631889.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260432
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7670.29451065921238556No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7290.2799195183387602No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6900.26494440007372366No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5970.22923450267248263No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5230.2008201757080543No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5230.2008201757080543No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5200.19966824353382073No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4860.18661301222584015No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4760.1827732383117282No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC4750.18238926092031701No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4500.17278982613503718No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG4040.15512686613012225No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC3990.15320697917306628No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3870.14859925047613196No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG3810.1462953861276648No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG3670.1409197026479081No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3380.12978435829698345No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT3370.12940038090557227No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3260.12517662960004916No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3190.12248878786017078No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA3170.1217208330773484No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3160.1213368556859372No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3070.11788105916323646No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3020.11596117220618049No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2980.11442526264053572No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2930.11250537568347975No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA2930.11250537568347975No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC2900.11135344350924618No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC2840.10904957916077901No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2760.10597776002948946No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2690.10328991828961111No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA2680.10290594089819992No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG2610.10021809915832156No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG2610.10021809915832156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGA200.001839323137.06
CACGACG200.001839323137.04
GTAATGT200.001839323137.06
ACGGACG251.2310053E-436.9999968
GTGAGTA303.591905E-430.8333322
TCACGAC250.00548863729.5999983
TGAGTAA250.00548863729.5999983
CCGCTTA250.00548863729.59999825
GCTTATT250.00548863729.59999827
GTGTAAG250.00548863729.5999981
CGGACGT250.00548863729.5999989
CTGTGCA652.9667717E-928.4615389
ACACGAA556.23053E-726.9090917
AAACACG556.23053E-726.9090915
GAACTGC358.850906E-426.4285726
GTGCAAA659.3335984E-825.61538511
GCAGTCG809.695214E-1025.43759
GGTGATT809.695214E-1025.437515
GGGCAGT809.695214E-1025.43757
CAGTCGG809.695214E-1025.437510
Produced by FastQC (version 0.11.5)