##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631889.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260432
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.613415402101126	34.0	31.0	34.0	31.0	34.0
2	32.78112520734779	34.0	31.0	34.0	31.0	34.0
3	32.869455366468024	34.0	31.0	34.0	31.0	34.0
4	36.29363519075997	37.0	37.0	37.0	35.0	37.0
5	36.216712999938565	37.0	35.0	37.0	35.0	37.0
6	36.26749784972661	37.0	37.0	37.0	35.0	37.0
7	36.25358634883578	37.0	37.0	37.0	35.0	37.0
8	36.23220264790809	37.0	37.0	37.0	35.0	37.0
9	38.08900979910303	39.0	38.0	39.0	37.0	39.0
10	38.012294956072985	39.0	38.0	39.0	35.0	39.0
11	38.10345886834183	39.0	38.0	39.0	37.0	39.0
12	38.01286708238619	39.0	38.0	39.0	35.0	39.0
13	38.07731000798673	39.0	38.0	39.0	37.0	39.0
14	39.459160164649504	40.0	39.0	41.0	37.0	41.0
15	39.4646011242858	41.0	39.0	41.0	37.0	41.0
16	39.43207055968544	40.0	39.0	41.0	37.0	41.0
17	39.3724158321558	40.0	39.0	41.0	37.0	41.0
18	39.3932043681268	40.0	39.0	41.0	37.0	41.0
19	39.37677397554832	40.0	39.0	41.0	37.0	41.0
20	39.407062112182835	40.0	39.0	41.0	37.0	41.0
21	39.376071296922035	40.0	39.0	41.0	37.0	41.0
22	39.3587846347607	40.0	39.0	41.0	36.0	41.0
23	39.29586993917798	40.0	39.0	41.0	36.0	41.0
24	39.30072341340542	40.0	39.0	41.0	36.0	41.0
25	39.25046845241752	40.0	39.0	41.0	36.0	41.0
26	39.18712754193033	40.0	39.0	41.0	36.0	41.0
27	39.09543758063525	40.0	39.0	41.0	36.0	41.0
28	39.075363242612276	40.0	39.0	41.0	36.0	41.0
29	39.04682604288259	40.0	39.0	41.0	35.0	41.0
30	39.01221048104688	40.0	39.0	41.0	35.0	41.0
31	38.89814999692818	40.0	38.0	41.0	35.0	41.0
32	38.86199084597899	40.0	38.0	41.0	35.0	41.0
33	38.79777830681329	40.0	38.0	41.0	35.0	41.0
34	38.749976961356516	40.0	38.0	41.0	35.0	41.0
35	38.687887817165326	40.0	38.0	41.0	35.0	41.0
36	38.63092477114947	40.0	38.0	41.0	35.0	41.0
37	38.57670716348221	40.0	38.0	41.0	35.0	41.0
38	38.537084536462494	40.0	38.0	41.0	35.0	41.0
39	38.45803511089267	40.0	38.0	41.0	34.0	41.0
40	38.10068271180193	40.0	37.0	41.0	34.0	41.0
41	38.180941666154695	40.0	37.0	41.0	34.0	41.0
42	38.167513976777045	40.0	37.0	41.0	34.0	41.0
43	37.68993057688763	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	4.0
21	6.0
22	29.0
23	42.0
24	107.0
25	206.0
26	336.0
27	508.0
28	826.0
29	1158.0
30	1776.0
31	2419.0
32	3351.0
33	4691.0
34	6583.0
35	9769.0
36	15020.0
37	28350.0
38	62025.0
39	123219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.044510659212385	15.762271917429501	12.22315230079253	27.970065122565586
2	20.631873195306262	18.76651102783068	32.5670424525404	28.034573324322665
3	21.311513178104075	18.736176813909196	28.83286232106654	31.119447686920193
4	14.812695828469618	15.715042698285925	33.917106960742146	35.5551545125023
5	17.3504024083062	32.18229710634638	33.4763009154021	16.990999569945323
6	35.733703999508506	32.83160287522271	15.50423911040118	15.930454014867603
7	31.718452417521657	26.7666799778829	19.63775572894268	21.877111875652762
8	28.390904343552254	29.89072003440437	20.324307304785894	21.39406831725748
9	28.999124531547583	11.617619954537076	17.243272716102474	42.13998279781286
10	19.048350433126497	22.977591079437243	29.492535479510966	28.48152300792529
11	40.42552374516188	18.614839958223257	17.737067641457273	23.222568655157584
12	23.075505314247096	23.363872335196902	25.72341340541869	27.83720894513731
13	35.95487497696136	16.856991460342815	20.578116360508695	26.610017202187137
14	24.95584259998771	19.71839098113903	22.8320636480924	32.49370277078086
15	29.702955090004302	23.4391319039135	20.22293727345334	26.634975732628863
16	27.207102045831544	22.77753885851201	22.1700866252995	27.845272470356946
17	27.626405357252565	23.036339620323155	21.04042513976777	28.29682988265651
18	27.300792529335872	20.140766111691345	23.009077225532963	29.54936413343982
19	29.4902316151625	22.0122719174295	22.350939976654175	26.146556490753824
20	31.406662775695764	19.66041039503594	22.22461141487989	26.7083154143884
21	27.578792160717576	21.930100755667507	20.982828531056093	29.508278552558824
22	30.193678196227808	21.721601032131225	20.40916630828777	27.675554463353198
23	27.285049456288014	21.712385574737358	21.96389076611169	29.038674202862936
24	28.215042698285924	21.16905756589052	22.439254776678748	28.176644959144802
25	28.06529151563556	21.05962400933833	22.97567119248019	27.899413282545925
26	28.675431590587948	22.205412545309333	21.072679240646313	28.046476623456414
27	28.24691282177305	20.904497143208207	22.922682312465444	27.925907722553294
28	26.345840756896234	22.633931314124226	21.620998955581495	29.399228973398046
29	28.66045647232291	22.988342446396757	22.08215580266634	26.269045278613994
30	28.094473797382808	20.379600049149104	22.444630460158503	29.081295693309578
31	27.46820667199115	21.53268415555692	22.61281255759661	28.386296614855315
32	26.12582171161762	22.030702832217237	22.183909811390308	29.659565644774837
33	26.412652823001782	20.815798365792222	24.377572648522456	28.393976162683543
34	27.29388093629047	21.319192725932297	23.799302697057197	27.587623640720032
35	26.941389690974994	22.5026110462616	23.489432942188365	27.06656632057505
36	26.021379861153775	21.625606684278427	23.850755667506295	28.502257787061495
37	25.916170055907106	21.785725256496896	25.320237144436934	26.977867543159057
38	26.11353443509246	20.697149351846164	24.539995085089387	28.649321127971984
39	25.30219020704061	20.337362536093874	27.053127111875654	27.30732014498986
40	26.159227744670392	20.92407999017018	26.40842907169626	26.508263193463165
41	23.193770350801746	20.14306997603981	28.001551268661302	28.66160840449714
42	21.333783866805923	20.94750261104626	29.389629538612766	28.329083983535046
43	21.480463230324997	20.867635313632732	28.472307550531422	29.179593905510842
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.0
2	0.0
3	5.0
4	10.0
5	10.0
6	14.5
7	19.0
8	20.0
9	21.0
10	36.5
11	52.0
12	52.0
13	93.0
14	134.0
15	277.0
16	420.0
17	406.5
18	393.0
19	393.0
20	433.0
21	473.0
22	381.0
23	289.0
24	330.0
25	371.0
26	371.0
27	368.5
28	366.0
29	511.0
30	656.0
31	865.5
32	1075.0
33	1075.0
34	1736.0
35	2397.0
36	2725.0
37	3053.0
38	3795.5
39	4538.0
40	4538.0
41	5342.5
42	6147.0
43	8033.0
44	9919.0
45	13908.5
46	17898.0
47	17898.0
48	20069.0
49	22240.0
50	25730.0
51	29220.0
52	30576.0
53	31932.0
54	31932.0
55	29384.5
56	26837.0
57	24736.5
58	22636.0
59	22132.0
60	21628.0
61	21628.0
62	19746.0
63	17864.0
64	14285.5
65	10707.0
66	9753.0
67	8799.0
68	8799.0
69	7830.5
70	6862.0
71	6865.0
72	6868.0
73	5552.5
74	4237.0
75	4237.0
76	2616.5
77	996.0
78	773.0
79	550.0
80	465.0
81	380.0
82	380.0
83	297.5
84	215.0
85	168.5
86	122.0
87	95.5
88	69.0
89	69.0
90	50.5
91	32.0
92	18.0
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	260432.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.06704208714511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.96229708810826	32.839868069883245
2	7.3940908410182	5.925186978465008
3	2.8014250283468614	3.367344435442661
4	1.51070782516467	2.4211837604900914
5	0.9909962489006764	1.9853144206453164
6	0.7821935096326038	1.880410816244477
7	0.5494323466936415	1.5409890271309123
8	0.44054473849605164	1.4121059662873308
9	0.36662112132340724	1.322048150829116
>10	2.633278671195855	21.054307168549176
>50	0.36362421709594994	10.080867273734317
>100	0.19779559061812663	14.429657193893267
>500	0.006992773405691345	1.740716738405088
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	767	0.29451065921238556	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	729	0.2799195183387602	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	690	0.26494440007372366	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	597	0.22923450267248263	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	523	0.2008201757080543	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	523	0.2008201757080543	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	520	0.19966824353382073	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	486	0.18661301222584015	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	476	0.1827732383117282	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	475	0.18238926092031701	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	450	0.17278982613503718	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	404	0.15512686613012225	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	399	0.15320697917306628	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	387	0.14859925047613196	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	381	0.1462953861276648	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	367	0.1409197026479081	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	338	0.12978435829698345	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	337	0.12940038090557227	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	326	0.12517662960004916	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	319	0.12248878786017078	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	317	0.1217208330773484	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	316	0.1213368556859372	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	307	0.11788105916323646	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	302	0.11596117220618049	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	298	0.11442526264053572	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	293	0.11250537568347975	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	293	0.11250537568347975	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	290	0.11135344350924618	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	284	0.10904957916077901	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	276	0.10597776002948946	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	269	0.10328991828961111	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	268	0.10290594089819992	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	261	0.10021809915832156	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	261	0.10021809915832156	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.839773914111937E-4	0.0	0.0	0.0	0.0
9	3.839773914111937E-4	0.0	0.0	3.839773914111937E-4	0.0
10	7.679547828223874E-4	0.0	0.0	3.839773914111937E-4	0.0
11	7.679547828223874E-4	0.0	0.0	3.839773914111937E-4	0.0
12	7.679547828223874E-4	0.0	0.0	3.839773914111937E-4	0.0
13	7.679547828223874E-4	0.0	0.0	7.679547828223874E-4	0.0
14	7.679547828223874E-4	0.0	0.0	7.679547828223874E-4	0.0
15	7.679547828223874E-4	0.0	0.0	7.679547828223874E-4	0.0
16	7.679547828223874E-4	0.0	0.0	0.0019198869570559685	0.0
17	0.0011519321742335812	0.0	0.0	0.0023038643484671623	0.0
18	0.0015359095656447747	0.0	0.0	0.0023038643484671623	0.0
19	0.0015359095656447747	0.0	0.0	0.0030718191312895495	0.0
20	0.0015359095656447747	0.0	0.0	0.003839773914111937	0.0
21	0.0015359095656447747	0.0	0.0	0.006143638262579099	0.0
22	0.0015359095656447747	0.0	0.0	0.008447502611046261	0.0
23	0.0015359095656447747	0.0	0.0	0.00921545739386865	0.0
24	0.0015359095656447747	0.0	0.0	0.01459114087362536	0.0
25	0.0015359095656447747	0.0	0.0	0.016895005222092523	0.0
26	0.0019198869570559685	0.0	0.0	0.01958284696197088	0.0
27	0.0019198869570559685	0.0	0.0	0.04031762609817534	0.0
28	0.0019198869570559685	0.0	0.0	0.1332401548196842	0.0
29	0.0019198869570559685	0.0	0.0	0.30641395834613255	0.0
30	0.0019198869570559685	0.0	0.0	0.5149136818824107	0.0
31	0.0019198869570559685	0.0	0.0	1.1480924003194692	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGA	20	0.0018393231	37.0	6
CACGACG	20	0.0018393231	37.0	4
GTAATGT	20	0.0018393231	37.0	6
ACGGACG	25	1.2310053E-4	36.999996	8
GTGAGTA	30	3.591905E-4	30.833332	2
TCACGAC	25	0.005488637	29.599998	3
TGAGTAA	25	0.005488637	29.599998	3
CCGCTTA	25	0.005488637	29.599998	25
GCTTATT	25	0.005488637	29.599998	27
GTGTAAG	25	0.005488637	29.599998	1
CGGACGT	25	0.005488637	29.599998	9
CTGTGCA	65	2.9667717E-9	28.461538	9
ACACGAA	55	6.23053E-7	26.90909	17
AAACACG	55	6.23053E-7	26.90909	15
GAACTGC	35	8.850906E-4	26.428572	6
GTGCAAA	65	9.3335984E-8	25.615385	11
GCAGTCG	80	9.695214E-10	25.4375	9
GGTGATT	80	9.695214E-10	25.4375	15
GGGCAGT	80	9.695214E-10	25.4375	7
CAGTCGG	80	9.695214E-10	25.4375	10
>>END_MODULE