FastQCFastQC Report
Fri 10 Feb 2017
ERR1631888.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631888.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155277
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4230.2724163913522286No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4000.2576041525789396No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC3750.24150389304275582No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3640.23441977884683501No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3500.22540363350657214No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3240.20865936358894105No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3040.19577915595999407No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2740.1764588445165736No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2660.17130676146499482No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2490.1603585849803899No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT2470.1590705642174952No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2420.15585051231025845No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2390.1539184811659164No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG1900.12236197247499629No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1860.1197859309492069No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1860.1197859309492069No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1800.1159218686605228No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1790.11527785827907545No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT1700.10948176484604931No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1690.10883775446460196No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1650.10626171293881258No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1620.10432968179447052No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG1600.10304166103157583No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1590.10239765065012847No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1580.10175364026868113No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCT303.5852808E-430.83333230
ATACGGC303.5852808E-430.83333229
GGTATCA2450.030.2040821
GCGGTAA1600.030.06250223
CGGTAAT1600.030.06250224
GTACACT250.00548189429.5999984
CACTGGT250.00548189429.59999834
CTGGTTA250.00548189429.59999836
TCAACCG250.00548189429.5999985
GGTAATA1850.028.025
AGTATTA405.9075974E-527.75000215
CAGTATT405.9075974E-527.75000214
CGTGCCA1750.027.48571610
GTGCCAG1850.027.011
AACTCCG1800.026.7222215
AGCCGCG1850.026.019
GTAATAC1850.026.026
TCCGTGC1850.026.08
TAATACG1800.025.69444527
CTCCGTG1900.025.315797
Produced by FastQC (version 0.11.5)