##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631888.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	155277
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58475498625038	34.0	31.0	34.0	31.0	34.0
2	32.76098198702963	34.0	31.0	34.0	31.0	34.0
3	32.839473972320434	34.0	31.0	34.0	31.0	34.0
4	36.265370917779194	37.0	37.0	37.0	35.0	37.0
5	36.194542656027615	37.0	35.0	37.0	35.0	37.0
6	36.24574792145649	37.0	37.0	37.0	35.0	37.0
7	36.23704734120314	37.0	37.0	37.0	35.0	37.0
8	36.20793807196172	37.0	36.0	37.0	35.0	37.0
9	38.06196023879905	39.0	38.0	39.0	35.0	39.0
10	37.97713763145862	39.0	38.0	39.0	35.0	39.0
11	38.06513520997959	39.0	38.0	39.0	35.0	39.0
12	37.98149758174102	39.0	38.0	39.0	35.0	39.0
13	38.040205568113755	39.0	38.0	39.0	35.0	39.0
14	39.42300533884606	40.0	39.0	41.0	37.0	41.0
15	39.422457930021835	40.0	39.0	41.0	37.0	41.0
16	39.37829169806217	40.0	39.0	41.0	36.0	41.0
17	39.337564481539445	40.0	39.0	41.0	36.0	41.0
18	39.34529260611681	40.0	39.0	41.0	36.0	41.0
19	39.35864294132421	40.0	39.0	41.0	36.0	41.0
20	39.346419624284344	40.0	39.0	41.0	36.0	41.0
21	39.31886886016603	40.0	39.0	41.0	36.0	41.0
22	39.28839428891594	40.0	39.0	41.0	36.0	41.0
23	39.22436677679244	40.0	39.0	41.0	36.0	41.0
24	39.24435685903257	40.0	39.0	41.0	36.0	41.0
25	39.17769534444895	40.0	39.0	41.0	36.0	41.0
26	39.10495437186447	40.0	39.0	41.0	35.0	41.0
27	39.00470127578456	40.0	39.0	41.0	35.0	41.0
28	38.98934162818705	40.0	39.0	41.0	35.0	41.0
29	38.959414465761185	40.0	38.0	41.0	35.0	41.0
30	38.92848908724409	40.0	38.0	41.0	35.0	41.0
31	38.85327511479485	40.0	38.0	41.0	35.0	41.0
32	38.82383096015508	40.0	38.0	41.0	35.0	41.0
33	38.75930756003787	40.0	38.0	41.0	35.0	41.0
34	38.72644371027261	40.0	38.0	41.0	35.0	41.0
35	38.63391229866625	40.0	38.0	41.0	35.0	41.0
36	38.60195006343502	40.0	38.0	41.0	35.0	41.0
37	38.563341641067254	40.0	38.0	41.0	35.0	41.0
38	38.52760550500074	40.0	38.0	41.0	34.0	41.0
39	38.459836292561036	40.0	38.0	41.0	34.0	41.0
40	38.12363711303026	40.0	37.0	41.0	34.0	41.0
41	38.214139891935055	40.0	37.0	41.0	34.0	41.0
42	38.21486118356228	40.0	37.0	41.0	34.0	41.0
43	37.74731608673532	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	2.0
21	11.0
22	24.0
23	42.0
24	76.0
25	138.0
26	208.0
27	321.0
28	478.0
29	777.0
30	1004.0
31	1506.0
32	1977.0
33	2855.0
34	4014.0
35	6379.0
36	9603.0
37	16182.0
38	37170.0
39	72505.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.37281116971606	15.664908518325316	12.099023036251344	28.863257275707284
2	21.271662899205936	18.24610212716629	32.03629642509837	28.445938548529405
3	20.956741822678183	19.313871339605996	28.84458097464531	30.884805863070515
4	15.597287428273344	15.099467403414543	33.71716352067595	35.586081647636156
5	16.969673551137646	32.53798051224586	33.40803853758123	17.08430739903527
6	35.28532879950025	33.39515832995228	15.062758811672044	16.256754058875426
7	31.255755842784183	27.174662055552336	19.61848825003059	21.951093851632887
8	28.282359911641773	30.335465007695923	19.709937724196113	21.672237356466187
9	29.080932784636488	12.146679804478449	16.629636069733444	42.142751341151616
10	19.75695048204177	22.763190942637994	28.95663878101715	28.523219794303085
11	40.17851967773721	18.711721632952724	18.159804736052347	22.949953953257726
12	24.431821840968077	23.000186763010618	25.35468871758213	27.213302678439177
13	35.09663375773617	17.09203552361264	19.881888496042556	27.929442222608632
14	24.289495546668213	19.089755726862318	22.940937807917464	33.67981091855201
15	30.14999001783909	23.015643012165356	19.6648569974948	27.169509972500755
16	26.188038151174997	23.210778157743903	21.12998061528752	29.471203075793582
17	27.557204222132057	23.544375535333632	20.684969441707402	28.213450800826912
18	27.031047740489576	20.525898877489908	22.83467609497865	29.608377287041865
19	28.798856237562546	21.584651944589346	22.17392144361367	27.442570374234432
20	30.155142100890664	20.096343953064526	22.17392144361367	27.574592502431138
21	28.88193357676926	21.169909258937253	20.924541303605814	29.023615860687674
22	29.91685825975515	22.275031073500905	19.999098385465974	27.809012281277973
23	28.90318591935702	22.01163082748894	20.929049376275945	28.156133876878098
24	29.200718715585694	20.86915641080134	21.523470958351847	28.406653915261117
25	28.75313150047979	20.869800421182788	22.403189139408926	27.973878938928497
26	29.266407774493324	21.82229177534342	21.627156629764873	27.28414382039838
27	28.410517977549798	20.89234078453345	22.904229216174965	27.792912021741788
28	26.701958435569985	21.900861041879995	21.960109996973152	29.437070525576875
29	28.136169555053225	22.54615944409024	21.938857654385387	27.37881334647114
30	27.140529505335625	20.623144445088453	23.635180999117704	28.601145050458214
31	27.91913805650547	21.97749827727223	22.256354772438932	27.84700889378337
32	26.128789196081843	21.521538927207505	22.484334447471294	29.865337429239357
33	26.741243068838273	21.55502746704277	23.766559116932964	27.937170347185997
34	27.371085221893775	21.461001951351456	23.10451644480509	28.06339638194968
35	26.875841238560767	22.846268281844704	23.97393045975901	26.30396001983552
36	27.068400342613526	21.598176162599742	23.873464840253224	27.459958654533512
37	26.43791417917657	21.855136304797234	24.08148019346072	27.625469322565483
38	26.690366248703928	20.710729856965294	24.835616350135563	27.763287544195215
39	25.415869703819627	20.11695228527084	26.17129388125737	28.295884129652173
40	25.192398101457396	20.62894053852148	27.340172723584306	26.838488636436818
41	23.429097677054553	19.649400748340064	28.142609658867702	28.77889191573768
42	21.515098823393032	20.768690791295555	29.05066429670846	28.66554608860295
43	21.384364715959222	20.361032219839384	29.202650746730036	29.051952317471358
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	2.5
4	4.0
5	4.0
6	4.0
7	4.0
8	8.0
9	12.0
10	17.0
11	22.0
12	22.0
13	42.0
14	62.0
15	126.0
16	190.0
17	180.5
18	171.0
19	171.0
20	189.5
21	208.0
22	163.5
23	119.0
24	138.5
25	158.0
26	158.0
27	186.0
28	214.0
29	289.5
30	365.0
31	511.5
32	658.0
33	658.0
34	1030.0
35	1402.0
36	1675.0
37	1948.0
38	2587.0
39	3226.0
40	3226.0
41	3806.5
42	4387.0
43	5461.5
44	6536.0
45	8578.5
46	10621.0
47	10621.0
48	11712.0
49	12803.0
50	14853.0
51	16903.0
52	17419.0
53	17935.0
54	17935.0
55	16831.0
56	15727.0
57	14698.5
58	13670.0
59	12852.0
60	12034.0
61	12034.0
62	11333.5
63	10633.0
64	8626.0
65	6619.0
66	5800.0
67	4981.0
68	4981.0
69	4236.5
70	3492.0
71	3259.0
72	3026.0
73	3185.5
74	3345.0
75	3345.0
76	2803.0
77	2261.0
78	1526.5
79	792.0
80	556.5
81	321.0
82	321.0
83	266.5
84	212.0
85	161.0
86	110.0
87	87.5
88	65.0
89	65.0
90	48.0
91	31.0
92	19.5
93	8.0
94	4.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	155277.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.16340475408464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.80709925105418	43.39019945001513
2	6.5542199005601365	6.706724112392692
3	2.436906035622129	3.7404122954462027
4	1.2977531625652967	2.655898813088867
5	0.842092013342564	2.154214725941382
6	0.6268487632953615	1.9243030197646787
7	0.49594058782805717	1.7761806320317886
8	0.36125621499150357	1.4786478358031132
9	0.3033545219963497	1.3968585173592998
>10	1.9812448863993959	20.226434050116886
>50	0.20894958776512054	7.284401424550964
>100	0.08433507457989804	7.26572512348899
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	423	0.2724163913522286	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	400	0.2576041525789396	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	375	0.24150389304275582	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	364	0.23441977884683501	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	350	0.22540363350657214	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	324	0.20865936358894105	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	304	0.19577915595999407	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	274	0.1764588445165736	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	266	0.17130676146499482	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	249	0.1603585849803899	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	247	0.1590705642174952	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	242	0.15585051231025845	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	239	0.1539184811659164	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	190	0.12236197247499629	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	186	0.1197859309492069	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	186	0.1197859309492069	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	180	0.1159218686605228	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	179	0.11527785827907545	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	170	0.10948176484604931	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	169	0.10883775446460196	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	165	0.10626171293881258	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	162	0.10432968179447052	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	160	0.10304166103157583	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	159	0.10239765065012847	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	158	0.10175364026868113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.44010381447349E-4	0.0	0.0	0.0	0.0
3	6.44010381447349E-4	0.0	0.0	0.0	0.0
4	6.44010381447349E-4	0.0	0.0	0.0	0.0
5	6.44010381447349E-4	0.0	0.0	0.0	0.0
6	6.44010381447349E-4	0.0	0.0	0.0	0.0
7	6.44010381447349E-4	0.0	0.0	0.0	0.0
8	6.44010381447349E-4	0.0	0.0	0.0	0.0
9	6.44010381447349E-4	0.0	0.0	0.0	0.0
10	6.44010381447349E-4	0.0	0.0	0.0	0.0
11	6.44010381447349E-4	0.0	0.0	0.0	0.0
12	6.44010381447349E-4	0.0	0.0	0.0	0.0
13	6.44010381447349E-4	0.0	0.0	6.44010381447349E-4	6.44010381447349E-4
14	6.44010381447349E-4	0.0	0.0	6.44010381447349E-4	6.44010381447349E-4
15	6.44010381447349E-4	0.0	0.0	6.44010381447349E-4	6.44010381447349E-4
16	6.44010381447349E-4	0.0	0.0	6.44010381447349E-4	6.44010381447349E-4
17	6.44010381447349E-4	0.0	0.0	0.001288020762894698	6.44010381447349E-4
18	0.001288020762894698	0.0	0.0	0.001288020762894698	6.44010381447349E-4
19	0.001288020762894698	0.0	0.0	0.001288020762894698	6.44010381447349E-4
20	0.001288020762894698	0.0	0.0	0.001288020762894698	6.44010381447349E-4
21	0.001288020762894698	0.0	0.0	0.002576041525789396	6.44010381447349E-4
22	0.001288020762894698	0.0	0.0	0.008372134958815535	6.44010381447349E-4
23	0.001288020762894698	0.0	0.0	0.011592186866052281	6.44010381447349E-4
24	0.001288020762894698	0.0	0.0	0.014168228391841677	6.44010381447349E-4
25	0.001288020762894698	0.0	0.0	0.016100259536183725	6.44010381447349E-4
26	0.001288020762894698	0.0	0.0	0.021252342587762516	6.44010381447349E-4
27	0.001288020762894698	0.0	0.0	0.03735260212394624	6.44010381447349E-4
28	0.001288020762894698	0.0	0.0	0.14232629429986413	6.44010381447349E-4
29	0.001288020762894698	0.0	0.0	0.31363305576485895	6.44010381447349E-4
30	0.001288020762894698	0.0	0.0	0.5139202843949845	6.44010381447349E-4
31	0.001288020762894698	0.0	0.0	1.0697012435840465	6.44010381447349E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGCT	30	3.5852808E-4	30.833332	30
ATACGGC	30	3.5852808E-4	30.833332	29
GGTATCA	245	0.0	30.204082	1
GCGGTAA	160	0.0	30.062502	23
CGGTAAT	160	0.0	30.062502	24
GTACACT	25	0.005481894	29.599998	4
CACTGGT	25	0.005481894	29.599998	34
CTGGTTA	25	0.005481894	29.599998	36
TCAACCG	25	0.005481894	29.599998	5
GGTAATA	185	0.0	28.0	25
AGTATTA	40	5.9075974E-5	27.750002	15
CAGTATT	40	5.9075974E-5	27.750002	14
CGTGCCA	175	0.0	27.485716	10
GTGCCAG	185	0.0	27.0	11
AACTCCG	180	0.0	26.722221	5
AGCCGCG	185	0.0	26.0	19
GTAATAC	185	0.0	26.0	26
TCCGTGC	185	0.0	26.0	8
TAATACG	180	0.0	25.694445	27
CTCCGTG	190	0.0	25.31579	7
>>END_MODULE