FastQCFastQC Report
Fri 10 Feb 2017
ERR1631887.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631887.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356566
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9300.26082127852908016No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9220.2585776546277548No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9090.25493176578810095No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC8270.23193462079951535No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8050.22576465507087046No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7430.20837656983559846No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6660.1867816897853413No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6210.1741613053403858No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5870.16462590375975275No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5780.16210182687076166No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5720.16041910894476757No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5520.15481004919145402No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4500.1262038444495549No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4440.12452112652356086No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4280.12003387872091002No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4270.11975342573324434No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4210.11807070780725026No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4040.11330300701693374No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3890.1090962122019486No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3670.10292624647330367No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3660.102645793485638No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3650.10236534049797232No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3580.10040216958431258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3450.031.637681
CAGGTTA250.005491323329.59999826
GTTCAAA250.005491323329.5999981
TTATTAC250.005491323329.5999981
TAATACT358.857352E-426.428574
ATACGGC809.731593E-1025.43750229
CTTATAC3050.024.8688537
ATCGAAA400.001928589223.12500224
GGTAATA3950.022.94936825
TAATACG3700.022.527
TTATGAG502.6970403E-422.1999993
AAAGTTG502.6970403E-422.19999928
CTAGTTC502.6970403E-422.1999993
AACTCCG4200.022.023815
GCGGTAA4050.021.92592623
GTAATAC4050.021.92592626
CGGTAAT4050.021.92592624
CGTGCCA4350.021.68965310
ACGTATA957.1031536E-921.4210532
CTAACTC4400.021.0227283
Produced by FastQC (version 0.11.5)