##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631887.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	356566
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.578950320557766	34.0	31.0	34.0	31.0	34.0
2	32.73638540971377	34.0	31.0	34.0	31.0	34.0
3	32.82593965773518	34.0	31.0	34.0	31.0	34.0
4	36.26498880992579	37.0	37.0	37.0	35.0	37.0
5	36.17884767476428	37.0	35.0	37.0	35.0	37.0
6	36.23046785167402	37.0	37.0	37.0	35.0	37.0
7	36.22130264803711	37.0	37.0	37.0	35.0	37.0
8	36.2013119590763	37.0	36.0	37.0	35.0	37.0
9	38.03563996567255	39.0	38.0	39.0	35.0	39.0
10	37.94786098506307	39.0	38.0	39.0	35.0	39.0
11	38.035530589007365	39.0	38.0	39.0	35.0	39.0
12	37.96766096599227	39.0	38.0	39.0	35.0	39.0
13	38.021721083894704	39.0	38.0	39.0	35.0	39.0
14	39.40943051216325	40.0	39.0	41.0	37.0	41.0
15	39.410914108468	40.0	39.0	41.0	37.0	41.0
16	39.362067611606264	40.0	39.0	41.0	36.0	41.0
17	39.31920598150132	40.0	39.0	41.0	36.0	41.0
18	39.33854041047099	40.0	39.0	41.0	36.0	41.0
19	39.357925320978445	40.0	39.0	41.0	36.0	41.0
20	39.35261354139206	40.0	39.0	41.0	36.0	41.0
21	39.31533012121178	40.0	39.0	41.0	36.0	41.0
22	39.301972145409266	40.0	39.0	41.0	36.0	41.0
23	39.234744759735925	40.0	39.0	41.0	36.0	41.0
24	39.24461390037188	40.0	39.0	41.0	36.0	41.0
25	39.19347329807105	40.0	39.0	41.0	36.0	41.0
26	39.12405557456404	40.0	39.0	41.0	36.0	41.0
27	39.004464811563636	40.0	39.0	41.0	35.0	41.0
28	38.99688416730704	40.0	39.0	41.0	35.0	41.0
29	38.988495818445955	40.0	39.0	41.0	35.0	41.0
30	38.96190887521525	40.0	38.0	41.0	35.0	41.0
31	38.86088129546844	40.0	38.0	41.0	35.0	41.0
32	38.82485991373266	40.0	38.0	41.0	35.0	41.0
33	38.761934676890114	40.0	38.0	41.0	35.0	41.0
34	38.73799520986297	40.0	38.0	41.0	35.0	41.0
35	38.66446043649703	40.0	38.0	41.0	35.0	41.0
36	38.617958526612185	40.0	38.0	41.0	35.0	41.0
37	38.584270513733784	40.0	38.0	41.0	35.0	41.0
38	38.540444125351264	40.0	38.0	41.0	35.0	41.0
39	38.461277855992996	40.0	38.0	41.0	34.0	41.0
40	38.152375717258515	40.0	38.0	41.0	34.0	41.0
41	38.225357998238756	40.0	37.0	41.0	34.0	41.0
42	38.22069406505388	40.0	37.0	41.0	34.0	41.0
43	37.7513756219045	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	6.0
19	9.0
20	19.0
21	40.0
22	64.0
23	95.0
24	184.0
25	320.0
26	502.0
27	722.0
28	1228.0
29	1779.0
30	2482.0
31	3337.0
32	4626.0
33	6384.0
34	9205.0
35	13818.0
36	20989.0
37	37842.0
38	87167.0
39	165745.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.84929017348822	16.174565157642622	12.645064307870072	28.331080360999085
2	20.67976195150407	19.586556205583257	32.213951975230394	27.51972986768228
3	20.75969105298879	19.89140860317585	29.252368425480835	30.096531918354525
4	15.456885962206155	15.732290796093851	34.48421891038405	34.32660433131594
5	16.361907753403298	33.47234453088629	33.82375212443138	16.341995591279034
6	34.20797271753336	34.30500945126568	15.567664892334099	15.919352938866856
7	30.41316334142908	27.801865573273954	20.11521008733306	21.66976099796391
8	27.518047149756285	30.95331579567317	20.516538312682645	21.012098741887897
9	28.68248795454418	12.549429839076076	17.31011930470095	41.45796290167879
10	18.928893949507245	23.618628809252705	29.740356624019114	27.712120617220936
11	39.3865371347801	19.366120157278036	18.7805343190321	22.46680838890977
12	22.90515640863122	23.718470072861685	26.764469971898606	26.611903546608485
13	34.92032330620418	17.524104934289866	21.078566099964664	26.47700565954129
14	24.00958027405866	19.588238923509252	23.151394131801688	33.2507866706304
15	29.47532855067505	24.13550366552055	19.881873201595216	26.507294582209184
16	25.51000375807003	24.299849116292634	22.084831419709115	28.105315705928213
17	27.26535900786951	23.85841611370686	21.490831991833208	27.38539288659042
18	26.475042488627633	21.536265375835047	23.32162909531475	28.66706304022257
19	28.194780208993564	22.224497007566622	23.312374146721783	26.268348636718024
20	30.11139592670081	20.551314483153188	22.75118771840276	26.58610187174324
21	27.155982342679895	22.138397940353258	21.83158237184701	28.874037345119834
22	28.43905476125037	22.6945362148943	21.01854916060421	27.847859863251124
23	28.072783159359	22.247774605542872	22.274417639371112	27.405024595727017
24	28.082599013927297	21.550568478205996	22.655272796621105	27.711559711245602
25	28.207120140450858	21.990879668841114	22.93712804922511	26.86487214148292
26	28.193377944055236	22.795779743441607	22.144007000106573	26.86683531239658
27	27.153177812803243	21.4459595138067	24.012665256922983	27.388197416467076
28	26.09811367320496	22.935445331299114	22.126058008895967	28.840382986599955
29	27.26367628994352	23.60853250169674	22.168406410033487	26.95938479832626
30	26.683699511450897	22.211596170134	23.54627193843496	27.55843237998014
31	27.836361290756827	22.611241677557594	22.778672111194002	26.773724920491578
32	25.550949894269227	22.311156980755317	22.838969503542124	29.29892362143334
33	26.135413920564492	21.897769276936106	24.347526124195802	27.6192906783036
34	26.591710931496554	21.801854355154447	23.81410454165568	27.79233017169332
35	25.927598256704226	23.706691047379728	24.27180381752607	26.093906878389976
36	26.2097339622959	22.518131285652586	24.658828940504705	26.613305811546812
37	26.98069922538885	22.21215707610933	24.103812477914328	26.703331220587494
38	26.035572656955512	21.797647560339463	25.16700975415491	26.999770028550113
39	25.448304100783588	20.903843888648947	25.842340548453862	27.805511462113607
40	25.121015464177738	21.80101299619145	26.82925461204938	26.248716927581427
41	23.438858444159006	20.866824094277074	27.879270597869677	27.81504686369424
42	22.274978545346443	21.215427157945513	28.609009271775772	27.900585024932273
43	21.456336274350328	21.81784017545139	28.75316210743593	27.972661442762348
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	5.5
4	11.0
5	11.0
6	20.0
7	29.0
8	29.0
9	29.0
10	37.0
11	45.0
12	45.0
13	102.5
14	160.0
15	347.5
16	535.0
17	540.5
18	546.0
19	546.0
20	604.5
21	663.0
22	565.0
23	467.0
24	583.0
25	699.0
26	699.0
27	824.5
28	950.0
29	1415.5
30	1881.0
31	2241.0
32	2601.0
33	2601.0
34	3284.5
35	3968.0
36	4642.5
37	5317.0
38	6939.5
39	8562.0
40	8562.0
41	10326.0
42	12090.0
43	13822.5
44	15555.0
45	20859.5
46	26164.0
47	26164.0
48	28833.5
49	31503.0
50	36272.0
51	41041.0
52	42299.0
53	43557.0
54	43557.0
55	39001.5
56	34446.0
57	32718.5
58	30991.0
59	28539.0
60	26087.0
61	26087.0
62	23970.5
63	21854.0
64	18221.0
65	14588.0
66	12694.0
67	10800.0
68	10800.0
69	9044.0
70	7288.0
71	6324.5
72	5361.0
73	4413.0
74	3465.0
75	3465.0
76	2847.0
77	2229.0
78	1803.0
79	1377.0
80	1073.0
81	769.0
82	769.0
83	596.5
84	424.0
85	338.0
86	252.0
87	197.5
88	143.0
89	143.0
90	113.5
91	84.0
92	54.0
93	24.0
94	16.0
95	8.0
96	8.0
97	4.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	356566.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.618626091319044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.0096073176457	43.53688153073086
2	6.387324857592261	6.466352173805006
3	2.2175807154849427	3.367526671933562
4	1.1648242365246737	2.358472099629945
5	0.7256662512860317	1.83661143204684
6	0.515345063222744	1.5651635437967548
7	0.375267533880967	1.3296868887212447
8	0.26772429532779335	1.0841468800607557
9	0.23433308549676177	1.0675456952027114
>10	1.7854050580601437	17.938004272796498
>50	0.20608257755158982	7.269991198323693
>100	0.10415524865445215	9.723394727673448
>500	0.006683759271943452	2.456222885278679
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	930	0.26082127852908016	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	922	0.2585776546277548	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	909	0.25493176578810095	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	827	0.23193462079951535	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	805	0.22576465507087046	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	743	0.20837656983559846	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	666	0.1867816897853413	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	621	0.1741613053403858	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	587	0.16462590375975275	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	578	0.16210182687076166	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	572	0.16041910894476757	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	552	0.15481004919145402	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	450	0.1262038444495549	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	444	0.12452112652356086	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	428	0.12003387872091002	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	427	0.11975342573324434	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	421	0.11807070780725026	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	404	0.11330300701693374	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	389	0.1090962122019486	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	367	0.10292624647330367	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	366	0.102645793485638	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	365	0.10236534049797232	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	358	0.10040216958431258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	2.804529876656776E-4	0.0
16	0.0	0.0	0.0	8.413589629970328E-4	2.804529876656776E-4
17	0.0	0.0	0.0	0.0016827179259940655	2.804529876656776E-4
18	2.804529876656776E-4	0.0	0.0	0.001963170913659743	2.804529876656776E-4
19	2.804529876656776E-4	0.0	0.0	0.0030849828643224535	2.804529876656776E-4
20	2.804529876656776E-4	0.0	0.0	0.005328606765647875	2.804529876656776E-4
21	2.804529876656776E-4	0.0	0.0	0.007572230666973295	2.804529876656776E-4
22	2.804529876656776E-4	0.0	0.0	0.01065721353129575	2.804529876656776E-4
23	2.804529876656776E-4	0.0	0.0	0.013742196395618203	2.804529876656776E-4
24	2.804529876656776E-4	0.0	0.0	0.016266273284609303	2.804529876656776E-4
25	2.804529876656776E-4	0.0	0.0	0.016827179259940657	2.804529876656776E-4
26	2.804529876656776E-4	0.0	0.0	0.019351256148931753	2.804529876656776E-4
27	2.804529876656776E-4	0.0	0.0	0.02916711071723047	2.804529876656776E-4
28	2.804529876656776E-4	0.0	0.0	0.08862314410235413	2.804529876656776E-4
29	2.804529876656776E-4	0.0	0.0	0.20473068099594466	2.804529876656776E-4
30	2.804529876656776E-4	0.0	0.0	0.35729710628607325	2.804529876656776E-4
31	2.804529876656776E-4	0.0	0.0	0.8096677753908113	2.804529876656776E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	345	0.0	31.63768	1
CAGGTTA	25	0.0054913233	29.599998	26
GTTCAAA	25	0.0054913233	29.599998	1
TTATTAC	25	0.0054913233	29.599998	1
TAATACT	35	8.857352E-4	26.42857	4
ATACGGC	80	9.731593E-10	25.437502	29
CTTATAC	305	0.0	24.86885	37
ATCGAAA	40	0.0019285892	23.125002	24
GGTAATA	395	0.0	22.949368	25
TAATACG	370	0.0	22.5	27
TTATGAG	50	2.6970403E-4	22.199999	3
AAAGTTG	50	2.6970403E-4	22.199999	28
CTAGTTC	50	2.6970403E-4	22.199999	3
AACTCCG	420	0.0	22.02381	5
GCGGTAA	405	0.0	21.925926	23
GTAATAC	405	0.0	21.925926	26
CGGTAAT	405	0.0	21.925926	24
CGTGCCA	435	0.0	21.689653	10
ACGTATA	95	7.1031536E-9	21.421053	2
CTAACTC	440	0.0	21.022728	3
>>END_MODULE