FastQCFastQC Report
Fri 10 Feb 2017
ERR1631886.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631886.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81887
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3260.39810959004481783No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3120.38101285918399747No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2990.36513732338466426No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2780.33949222709343363No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2550.3114047406792287No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2520.3077411554947672No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2440.2979715950028698No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2080.25400857278933164No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2070.2527873777278445No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1920.2344694518055369No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1890.23080586662107536No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1870.22836347649810104No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1490.18195806416158852No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1470.17951567403861418No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1460.17829447897712702No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1390.16974611354671681No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1350.16486133330076813No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1340.16364013823928097No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1270.15509177280887076No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT1250.15264938268589642No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1240.15142818762440924No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1230.15020699256292208No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1220.1489857975014349No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1180.14410101725548624No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1180.14410101725548624No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC1160.14165862713251187No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1130.13799504194805035No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA1090.1331102617021017No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1090.1331102617021017No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1070.13066787157912735No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1060.12944667651764016No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC990.12089831108722997No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC990.12089831108722997No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC970.11845592096425563No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG960.11723472590276846No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG950.11601353084128127No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC950.11601353084128127No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA950.11601353084128127No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA950.11601353084128127No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA950.11601353084128127No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA940.11479233577979411No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC940.11479233577979411No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG930.11357114071830693No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG930.11357114071830693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT910.1111287505953326No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC910.1111287505953326No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT910.1111287505953326No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA900.10990755553384542No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG890.10868636047235826No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC870.1062439703493839No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC870.1062439703493839No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG860.10502277528789675No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT850.10380158022640956No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC850.10380158022640956No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA850.10380158022640956No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG850.10380158022640956No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA820.10013799504194805No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA820.10013799504194805No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG820.10013799504194805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCAC200.001831967237.026
CACCTCT200.001831967237.030
ACCTCTA200.001831967237.031
ATACGGC352.3615105E-531.71428729
ATCTTAC250.005466938529.5999981
TTACCCC250.005466938529.5999984
TTACCAA250.005466938529.59999817
CACCGTA250.005466938529.5999987
ACCCCGC250.005466938529.5999986
AAAACAT250.005466938529.59999823
TTTACCA250.005466938529.59999816
TCTTACC250.005466938529.5999982
GCGGTAA950.029.21052723
TACGGCT405.874013E-527.75000230
ACGGCTG405.874013E-527.75000231
GGTATCA1400.027.7500021
AGCCGCG1100.026.9090919
GGTGATT358.798915E-426.42857215
TGATTCC358.798915E-426.42857217
GTACTGG451.3086025E-424.6666681
Produced by FastQC (version 0.11.5)