##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631886.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	81887
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61575097390306	34.0	31.0	34.0	31.0	34.0
2	32.786315288140976	34.0	31.0	34.0	31.0	34.0
3	32.87319110481517	34.0	31.0	34.0	31.0	34.0
4	36.28656563312858	37.0	37.0	37.0	35.0	37.0
5	36.21101029467437	37.0	35.0	37.0	35.0	37.0
6	36.272717281131314	37.0	37.0	37.0	35.0	37.0
7	36.27888431619183	37.0	37.0	37.0	35.0	37.0
8	36.26132353120764	37.0	37.0	37.0	35.0	37.0
9	38.08636291474837	39.0	38.0	39.0	37.0	39.0
10	38.014849731947685	39.0	38.0	39.0	35.0	39.0
11	38.09936864215321	39.0	38.0	39.0	37.0	39.0
12	38.027183802068706	39.0	38.0	39.0	35.0	39.0
13	38.08716890348895	39.0	38.0	39.0	37.0	39.0
14	39.45324654707096	40.0	39.0	41.0	37.0	41.0
15	39.47644925323922	41.0	39.0	41.0	37.0	41.0
16	39.41481553848596	40.0	39.0	41.0	37.0	41.0
17	39.39577710747738	40.0	39.0	41.0	37.0	41.0
18	39.4345500506796	40.0	39.0	41.0	37.0	41.0
19	39.445137811862686	41.0	39.0	41.0	37.0	41.0
20	39.465263106475994	41.0	39.0	41.0	37.0	41.0
21	39.424951457496306	40.0	39.0	41.0	37.0	41.0
22	39.39950175241491	40.0	39.0	41.0	37.0	41.0
23	39.352375834992124	40.0	39.0	41.0	37.0	41.0
24	39.36307350373075	40.0	39.0	41.0	36.0	41.0
25	39.29474764004054	40.0	39.0	41.0	36.0	41.0
26	39.23648442365699	40.0	39.0	41.0	36.0	41.0
27	39.11766214417429	40.0	39.0	41.0	36.0	41.0
28	39.1224370168647	40.0	39.0	41.0	36.0	41.0
29	39.10383821607825	40.0	39.0	41.0	36.0	41.0
30	39.08980668482177	40.0	39.0	41.0	35.0	41.0
31	38.98762929402714	40.0	39.0	41.0	35.0	41.0
32	38.965183728797	40.0	38.0	41.0	35.0	41.0
33	38.89072746589813	40.0	38.0	41.0	35.0	41.0
34	38.8824111275294	40.0	38.0	41.0	35.0	41.0
35	38.82002027183802	40.0	38.0	41.0	35.0	41.0
36	38.78122290473458	40.0	38.0	41.0	35.0	41.0
37	38.73537924212634	40.0	38.0	41.0	35.0	41.0
38	38.69145285576465	40.0	38.0	41.0	35.0	41.0
39	38.60571275049764	40.0	38.0	41.0	35.0	41.0
40	38.30125660971827	40.0	38.0	41.0	34.0	41.0
41	38.36044793434856	40.0	38.0	41.0	34.0	41.0
42	38.35890922857108	40.0	38.0	41.0	34.0	41.0
43	37.887259271923504	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	4.0
22	5.0
23	24.0
24	37.0
25	48.0
26	98.0
27	146.0
28	222.0
29	363.0
30	516.0
31	692.0
32	971.0
33	1333.0
34	1953.0
35	3002.0
36	4484.0
37	8255.0
38	20122.0
39	39610.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.25090673733315	17.185878100308962	12.737064491311198	27.826150671046683
2	20.892205111922525	19.787023581276635	32.775654255254196	26.545117051546647
3	20.43303576880335	20.652850879871043	30.010868636047235	28.90324471527837
4	13.898420994785496	16.973390159610194	38.054880506063235	31.07330833954107
5	15.017035671107745	33.779476595796645	35.86772015093971	15.3357675821559
6	29.97056919901816	37.488245997533184	16.54597188808968	15.99521291535897
7	28.318292280826018	28.318292280826018	21.57729554141683	21.786119896931137
8	25.634105535677215	30.83761769267405	21.567525980924934	21.9607507907238
9	27.977578858671098	12.495267869136738	18.601243176572595	40.92591009561957
10	19.160550514733718	22.69468902267759	30.318609791542002	27.826150671046683
11	38.40169990352559	19.995847936790945	19.620941052914382	21.981511106769084
12	21.68964548707365	25.1212036098526	26.98230488355905	26.206846019514696
13	34.42426758826188	18.504768766715106	20.814048627987347	26.25691501703567
14	22.615311343680926	21.436858109345806	24.184546997691942	31.76328354928133
15	29.67626118919975	24.079524222404043	21.42708854885391	24.817126039542295
16	24.1882105828764	24.851319501263937	23.82307325949174	27.137396656367923
17	26.16776777754711	24.47763381244886	21.786119896931137	27.56847851307289
18	25.171272607373574	22.1988838277138	24.342081160623785	28.287762404288834
19	27.04824941687936	22.781393872043182	24.72431521486927	25.44604149620819
20	29.562690048481443	21.00699744770232	23.704617338527484	25.725695165288755
21	26.07251456275111	22.683698267124207	22.787499847350617	28.456287322774067
22	28.02154188088463	23.794985773077535	21.66766397596688	26.515808370070953
23	27.32423950077546	22.967015521389232	23.241784410223847	26.466960567611466
24	27.018940735403667	22.664159146140413	23.163627926288665	27.153272192167254
25	27.700367579713507	22.248952825234774	24.132035610047993	25.918643985003726
26	26.847973426795463	24.064869881666198	23.2173605089941	25.869796182544235
27	26.08961129361193	22.371072331383495	25.11876121972963	26.42055515527495
28	24.891618938293014	23.731483629880202	23.163627926288665	28.21326950553812
29	27.24364062671731	24.39703493839071	23.275977871945486	25.083346562946502
30	26.143343876317367	21.74093567965611	25.204244874033733	26.911475569992792
31	27.145945021798333	23.600815758301074	23.94885635082492	25.304382869075674
32	24.995420518519424	22.76185475105939	23.558073931149025	28.684650799272166
33	24.889176548170038	22.21109577832867	25.827054355392185	27.072673318109103
34	25.938183105987516	22.45777718074908	25.17371499749655	26.43032471576685
35	24.861089061755834	25.123645999975576	24.968554227166706	25.046710711101884
36	25.925971155372647	23.461599521291536	25.660971827029933	24.951457496305885
37	26.302099234310695	23.538534810165228	24.91482164446127	25.244544311062807
38	25.58281534309475	22.44922881531867	25.492446908544704	26.475508933041876
39	24.484960982817785	22.321003333862517	26.398573644168184	26.795462039151513
40	24.98931454321199	23.207590948502204	27.15205099710577	24.651043511180042
41	22.653168390587027	21.936326889494058	28.01787829570017	27.39262642421874
42	20.91785020821376	23.039066030016976	28.90812949552432	27.134954266244947
43	21.252457655061242	23.203927363317742	28.20960592035366	27.334009061267356
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	2.5
7	4.0
8	7.0
9	10.0
10	10.5
11	11.0
12	11.0
13	32.0
14	53.0
15	91.5
16	130.0
17	117.0
18	104.0
19	104.0
20	127.0
21	150.0
22	114.5
23	79.0
24	68.0
25	57.0
26	57.0
27	73.5
28	90.0
29	143.0
30	196.0
31	312.0
32	428.0
33	428.0
34	689.5
35	951.0
36	1158.0
37	1365.0
38	1853.0
39	2341.0
40	2341.0
41	2852.0
42	3363.0
43	4101.5
44	4840.0
45	6203.5
46	7567.0
47	7567.0
48	8090.0
49	8613.0
50	9942.5
51	11272.0
52	11008.0
53	10744.0
54	10744.0
55	9532.5
56	8321.0
57	7436.5
58	6552.0
59	5906.5
60	5261.0
61	5261.0
62	4621.0
63	3981.0
64	3056.0
65	2131.0
66	1768.0
67	1405.0
68	1405.0
69	1135.5
70	866.0
71	688.0
72	510.0
73	368.5
74	227.0
75	227.0
76	174.5
77	122.0
78	99.5
79	77.0
80	55.5
81	34.0
82	34.0
83	28.0
84	22.0
85	14.0
86	6.0
87	4.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	81887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.91353938964671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.53800024476807	29.75075408795047
2	10.30779586341941	8.22841232430056
3	4.45171949577775	5.330516443391503
4	2.3222371802716926	3.7075482066750522
5	1.511442907844817	3.0163518018733133
6	1.1657079916778852	2.7916519105596738
7	0.8138538734549015	2.273865204489113
8	0.7128870395300453	2.276307594612087
9	0.4772977603720475	1.7145578663279883
>10	3.2829519030718393	25.28728613821486
>50	0.32125810794272425	9.13087547473958
>100	0.09484763186880431	6.4918729468658025
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	326	0.39810959004481783	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	312	0.38101285918399747	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	299	0.36513732338466426	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	278	0.33949222709343363	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	255	0.3114047406792287	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	252	0.3077411554947672	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	244	0.2979715950028698	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	208	0.25400857278933164	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	207	0.2527873777278445	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	192	0.2344694518055369	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	189	0.23080586662107536	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	187	0.22836347649810104	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	149	0.18195806416158852	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	147	0.17951567403861418	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	146	0.17829447897712702	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	139	0.16974611354671681	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	135	0.16486133330076813	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	134	0.16364013823928097	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	127	0.15509177280887076	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	125	0.15264938268589642	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	124	0.15142818762440924	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	123	0.15020699256292208	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	122	0.1489857975014349	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	118	0.14410101725548624	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	118	0.14410101725548624	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	116	0.14165862713251187	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	113	0.13799504194805035	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	109	0.1331102617021017	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	109	0.1331102617021017	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	107	0.13066787157912735	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	106	0.12944667651764016	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	99	0.12089831108722997	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	99	0.12089831108722997	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	97	0.11845592096425563	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	96	0.11723472590276846	No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG	95	0.11601353084128127	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	95	0.11601353084128127	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	95	0.11601353084128127	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	95	0.11601353084128127	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	95	0.11601353084128127	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	94	0.11479233577979411	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	94	0.11479233577979411	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	93	0.11357114071830693	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	93	0.11357114071830693	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	91	0.1111287505953326	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	91	0.1111287505953326	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	91	0.1111287505953326	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	90	0.10990755553384542	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	89	0.10868636047235826	No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC	87	0.1062439703493839	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	87	0.1062439703493839	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	86	0.10502277528789675	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	85	0.10380158022640956	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	85	0.10380158022640956	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	85	0.10380158022640956	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATG	85	0.10380158022640956	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	82	0.10013799504194805	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	82	0.10013799504194805	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	82	0.10013799504194805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0012211950614871713	0.0	0.0	0.0	0.0
7	0.0012211950614871713	0.0	0.0	0.0	0.0
8	0.0012211950614871713	0.0	0.0	0.0	0.0
9	0.0012211950614871713	0.0	0.0	0.0	0.0
10	0.0012211950614871713	0.0	0.0	0.0	0.0
11	0.0012211950614871713	0.0	0.0	0.0	0.0
12	0.0012211950614871713	0.0	0.0	0.0	0.0
13	0.0012211950614871713	0.0	0.0	0.0	0.0
14	0.0012211950614871713	0.0	0.0	0.0	0.0
15	0.0012211950614871713	0.0	0.0	0.0	0.0
16	0.0012211950614871713	0.0	0.0	0.0012211950614871713	0.0
17	0.0012211950614871713	0.0	0.0	0.0012211950614871713	0.0
18	0.0012211950614871713	0.0	0.0	0.0012211950614871713	0.0
19	0.0012211950614871713	0.0	0.0	0.0012211950614871713	0.0
20	0.0012211950614871713	0.0	0.0	0.0036635851844615142	0.0
21	0.0012211950614871713	0.0	0.0	0.006105975307435857	0.0
22	0.0012211950614871713	0.0	0.0	0.0085483654304102	0.0
23	0.0012211950614871713	0.0	0.0	0.012211950614871714	0.0
24	0.0012211950614871713	0.0	0.0	0.013433145676358884	0.0
25	0.0012211950614871713	0.0	0.0	0.014654340737846057	0.0
26	0.0012211950614871713	0.0	0.0	0.024423901229743427	0.0
27	0.0012211950614871713	0.0	0.0	0.03907824196758948	0.0012211950614871713
28	0.0012211950614871713	0.0	0.0	0.09647440985748654	0.0012211950614871713
29	0.0012211950614871713	0.0	0.0	0.19294881971497307	0.0012211950614871713
30	0.0012211950614871713	0.0	0.0	0.341934617216408	0.0012211950614871713
31	0.0012211950614871713	0.0	0.0	0.7620257183679949	0.0012211950614871713
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATCAC	20	0.0018319672	37.0	26
CACCTCT	20	0.0018319672	37.0	30
ACCTCTA	20	0.0018319672	37.0	31
ATACGGC	35	2.3615105E-5	31.714287	29
ATCTTAC	25	0.0054669385	29.599998	1
TTACCCC	25	0.0054669385	29.599998	4
TTACCAA	25	0.0054669385	29.599998	17
CACCGTA	25	0.0054669385	29.599998	7
ACCCCGC	25	0.0054669385	29.599998	6
AAAACAT	25	0.0054669385	29.599998	23
TTTACCA	25	0.0054669385	29.599998	16
TCTTACC	25	0.0054669385	29.599998	2
GCGGTAA	95	0.0	29.210527	23
TACGGCT	40	5.874013E-5	27.750002	30
ACGGCTG	40	5.874013E-5	27.750002	31
GGTATCA	140	0.0	27.750002	1
AGCCGCG	110	0.0	26.90909	19
GGTGATT	35	8.798915E-4	26.428572	15
TGATTCC	35	8.798915E-4	26.428572	17
GTACTGG	45	1.3086025E-4	24.666668	1
>>END_MODULE