FastQCFastQC Report
Fri 10 Feb 2017
ERR1631885.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631885.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842724
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13210.15675357531054057No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12860.1526003768730925No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12610.14963380656062958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11520.13669955999829125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9890.11735752156103302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9840.11676420749854044No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8680.1029993212487125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8600.029.6860471
TAGACAG601.3367007E-624.6666665
GACGTAT400.001930761823.12528
TATACAC2850.022.71929737
CTTATAC10250.020.75609837
ATACTTA450.00382497420.5555556
ACGTATA450.00382497420.55555529
TTTTACG656.899335E-519.9230774
GCGGTAA4800.019.6562523
CGCGGTA5050.018.68316822
GGACGTA609.234031E-418.527
TCTATAA500.007033363518.4999982
GGTAATA5650.018.0088525
CGGTAAT5350.017.63551524
ATACGGC1054.79351E-717.6190529
GTATCAA14550.017.5463922
GCCTAGT953.6040765E-617.5263161
TCACGTA953.6040765E-617.52631625
AGACAGT852.7217196E-517.4117646
TCTTATA19450.017.31105637
Produced by FastQC (version 0.11.5)