##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631885.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	842724
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63997465362325	34.0	31.0	34.0	31.0	34.0
2	32.80881166313051	34.0	31.0	34.0	31.0	34.0
3	32.90252680592934	34.0	31.0	34.0	31.0	34.0
4	36.30759892918678	37.0	37.0	37.0	35.0	37.0
5	36.22928621945026	37.0	35.0	37.0	35.0	37.0
6	36.2820769314746	37.0	37.0	37.0	35.0	37.0
7	36.27047289504037	37.0	37.0	37.0	35.0	37.0
8	36.25126257232498	37.0	37.0	37.0	35.0	37.0
9	38.08656096183329	39.0	38.0	39.0	37.0	39.0
10	38.02409092419345	39.0	38.0	39.0	35.0	39.0
11	38.0962023153488	39.0	38.0	39.0	37.0	39.0
12	38.03853812161514	39.0	38.0	39.0	35.0	39.0
13	38.08228791395522	39.0	38.0	39.0	37.0	39.0
14	39.49484054091256	41.0	39.0	41.0	37.0	41.0
15	39.50520336432806	41.0	39.0	41.0	37.0	41.0
16	39.46514873196919	41.0	39.0	41.0	37.0	41.0
17	39.42676961852279	41.0	39.0	41.0	37.0	41.0
18	39.447403894988156	41.0	39.0	41.0	37.0	41.0
19	39.473551245722206	41.0	39.0	41.0	37.0	41.0
20	39.4657717117348	41.0	39.0	41.0	37.0	41.0
21	39.429163047450885	41.0	39.0	41.0	37.0	41.0
22	39.40556813381368	41.0	39.0	41.0	37.0	41.0
23	39.34116270570198	40.0	39.0	41.0	36.0	41.0
24	39.36119061519548	41.0	39.0	41.0	36.0	41.0
25	39.30907509457426	41.0	39.0	41.0	36.0	41.0
26	39.255395598084306	40.0	39.0	41.0	36.0	41.0
27	39.147063570041915	40.0	39.0	41.0	36.0	41.0
28	39.13959018611075	40.0	39.0	41.0	36.0	41.0
29	39.117944902482904	40.0	39.0	41.0	35.0	41.0
30	39.0859676477708	40.0	39.0	41.0	35.0	41.0
31	38.997639796659406	40.0	39.0	41.0	35.0	41.0
32	38.949967011738124	40.0	38.0	41.0	35.0	41.0
33	38.90268818735434	40.0	38.0	41.0	35.0	41.0
34	38.86592882129855	40.0	38.0	41.0	35.0	41.0
35	38.805340775864934	40.0	38.0	41.0	35.0	41.0
36	38.75125426592811	40.0	38.0	41.0	35.0	41.0
37	38.71524366221919	40.0	38.0	41.0	35.0	41.0
38	38.673455366169705	40.0	38.0	41.0	35.0	41.0
39	38.61659570630479	40.0	38.0	41.0	35.0	41.0
40	38.3003426982025	40.0	38.0	41.0	34.0	41.0
41	38.37230813409847	40.0	38.0	41.0	34.0	41.0
42	38.36459386465794	40.0	38.0	41.0	34.0	41.0
43	37.911943886729226	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	10.0
19	12.0
20	43.0
21	70.0
22	130.0
23	219.0
24	389.0
25	617.0
26	1074.0
27	1632.0
28	2422.0
29	3770.0
30	5417.0
31	7328.0
32	9985.0
33	13696.0
34	20217.0
35	30492.0
36	47063.0
37	84926.0
38	192777.0
39	420432.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.96851638258789	16.995481320100055	12.741300829215735	27.294701468096317
2	19.926096800375923	19.842795506001963	34.13383266644833	26.097275027173783
3	20.56177348693048	20.470521784119118	29.516306643693547	29.45139808525686
4	15.226218785747173	15.894646408551317	34.13751121363578	34.74162359206573
5	16.343903816670704	33.905169426763685	33.489374931768886	16.26155182479673
6	34.17097412676036	34.22330442707221	15.604515831992444	16.001205614174985
7	30.205144270247438	28.673088698079084	20.014500595687316	21.10726643598616
8	26.84864795591439	32.28435407084645	20.206734351934916	20.66026362130425
9	27.66635339684167	13.780787066702741	17.980619989462742	40.57223954699285
10	18.66328714976671	24.564270152505447	30.606936553367415	26.165506144360428
11	37.99262866608759	20.428871136932138	20.058049847874273	21.520450349105992
12	22.58734769628016	24.034915345949564	27.694476483403818	25.68326047436646
13	33.50658103958117	18.253188469771835	22.3039809000337	25.936249590613297
14	23.695421039391306	19.832471841314593	24.011538771887356	32.46056834740674
15	28.214694253397315	25.036548146249544	20.914676691301068	25.834080909052076
16	25.317304360621034	24.620397662817243	22.77352964909033	27.28876832747139
17	26.054081763424325	24.231776951884605	22.97430712783782	26.739834156853252
18	25.803821891864953	22.522795126280965	24.379630816257755	27.29375216559633
19	27.54780924715565	23.101039011586234	24.093297449698834	25.25785429155928
20	28.42045557026974	22.118392261286022	23.758905644078013	25.702246524366224
21	26.66472059654169	23.33729667127078	22.87676629596404	27.121216436223484
22	26.85220784028935	23.760092272203	22.50297843659371	26.88472145091394
23	26.762024102790477	23.30062986220874	23.378235341582773	26.55911069341801
24	27.09819585059877	22.733658944090827	23.469843032831626	26.698302172478773
25	27.239523260284503	22.969916603775374	23.77990896189025	26.010651174049865
26	26.949748672163125	23.217091242209786	23.49749146814378	26.335668617483304
27	26.490879576231364	22.85789890877678	24.30641586094617	26.34480565404569
28	25.889852430926375	23.94152771251323	23.32139585439598	26.84722400216441
29	26.25367261404683	24.236048813134552	23.24153578158448	26.26874279123414
30	26.032129143112098	23.85846374376427	24.12153919907348	25.98786791405015
31	26.906555408413666	23.19039210939762	23.610814454079865	26.292238028108848
32	24.469221239694136	23.437804073457027	24.295498882196306	27.79747580465253
33	25.243614754059458	22.869527864401633	25.24919190624689	26.637665475292028
34	25.356463088745546	22.626269098779673	25.065620535311677	26.951647277163104
35	25.328577327808393	23.635970970329552	25.0316829709371	26.003768730924953
36	25.29202918155885	22.955083752213064	25.65679866717929	26.0960883990488
37	25.97089913186286	22.08718394159891	25.101100716248737	26.840816210289493
38	24.599394345005006	21.901120651601236	26.575248835917808	26.924236167475947
39	24.267494458446656	21.02301583911221	27.551962445593098	27.15752725684803
40	23.973210683450336	21.56376227566795	28.409657254332377	26.053369786549336
41	22.88697129783891	21.194127614735073	29.18476274557269	26.734138341853324
42	22.12254545972347	21.266986581609164	29.579909911192754	27.030558047474617
43	21.109402366611132	21.73048352722837	29.655616785566806	27.504497320593696
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	13.5
2	12.0
3	37.5
4	63.0
5	63.0
6	86.5
7	110.0
8	119.0
9	128.0
10	205.0
11	282.0
12	282.0
13	542.0
14	802.0
15	1588.0
16	2374.0
17	2438.5
18	2503.0
19	2503.0
20	2713.5
21	2924.0
22	2921.0
23	2918.0
24	3346.5
25	3775.0
26	3775.0
27	4455.5
28	5136.0
29	7154.0
30	9172.0
31	10415.0
32	11658.0
33	11658.0
34	14035.0
35	16412.0
36	18084.5
37	19757.0
38	23782.0
39	27807.0
40	27807.0
41	32437.0
42	37067.0
43	40256.0
44	43445.0
45	52527.0
46	61609.0
47	61609.0
48	67696.0
49	73783.0
50	79456.5
51	85130.0
52	87773.0
53	90416.0
54	90416.0
55	82652.5
56	74889.0
57	70180.0
58	65471.0
59	60035.5
60	54600.0
61	54600.0
62	50146.0
63	45692.0
64	38610.5
65	31529.0
66	27713.5
67	23898.0
68	23898.0
69	20725.5
70	17553.0
71	15048.0
72	12543.0
73	10096.0
74	7649.0
75	7649.0
76	6296.0
77	4943.0
78	4006.5
79	3070.0
80	2385.5
81	1701.0
82	1701.0
83	1331.5
84	962.0
85	746.0
86	530.0
87	393.5
88	257.0
89	257.0
90	177.5
91	98.0
92	65.5
93	33.0
94	19.0
95	5.0
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	842724.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.96239405147294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.21705220198726	48.48620385184983
2	5.903413922005992	6.489315244604894
3	1.8065919522324898	2.9788385630646568
4	0.9452627084856933	2.0781560586381307
5	0.5414425347449248	1.4879488975439445
6	0.39707386684505186	1.3094478202247903
7	0.28559371467856565	1.098783999935108
8	0.21182414189346815	0.9313889565091132
9	0.17947693075120455	0.8878033611986963
>10	1.2369044307485142	13.652912652251128
>50	0.15921546110957088	6.118899770037984
>100	0.10810041733258902	11.275141401605273
>500	0.007177693705228929	2.6050363274959825
>1k	8.700234794216884E-4	0.6001230950406053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1321	0.15675357531054057	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1286	0.1526003768730925	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1261	0.14963380656062958	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1152	0.13669955999829125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	989	0.11735752156103302	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	984	0.11676420749854044	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	868	0.1029993212487125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	1.1866281249851672E-4	0.0	0.0	0.0
12	0.0	1.1866281249851672E-4	0.0	1.1866281249851672E-4	0.0
13	0.0	1.1866281249851672E-4	0.0	1.1866281249851672E-4	0.0
14	0.0	1.1866281249851672E-4	0.0	1.1866281249851672E-4	0.0
15	0.0	1.1866281249851672E-4	0.0	7.119768749911003E-4	0.0
16	0.0	1.1866281249851672E-4	0.0	0.001305290937483684	0.0
17	0.0	1.1866281249851672E-4	0.0	0.0018986049999762675	0.0
18	0.0	1.1866281249851672E-4	0.0	0.002491919062468851	0.0
19	0.0	1.1866281249851672E-4	0.0	0.0032038959374599513	0.0
20	1.1866281249851672E-4	1.1866281249851672E-4	0.0	0.004509186874943635	0.0
21	1.1866281249851672E-4	1.1866281249851672E-4	0.0	0.007001105937412486	0.0
22	1.1866281249851672E-4	1.1866281249851672E-4	0.0	0.009849013437376887	0.0
23	1.1866281249851672E-4	1.1866281249851672E-4	0.0	0.012222269687347222	0.0
24	1.1866281249851672E-4	1.1866281249851672E-4	0.0	0.014239537499822005	0.0
25	1.1866281249851672E-4	1.1866281249851672E-4	0.0	0.016138142499798273	0.0
26	1.1866281249851672E-4	1.1866281249851672E-4	0.0	0.018511398749768607	0.0
27	1.1866281249851672E-4	1.1866281249851672E-4	0.0	0.03358157593708023	0.0
28	1.1866281249851672E-4	1.1866281249851672E-4	0.0	0.12661322093591734	0.0
29	1.1866281249851672E-4	1.1866281249851672E-4	0.0	0.2706698753091166	0.0
30	1.1866281249851672E-4	1.1866281249851672E-4	0.0	0.4663448531191707	0.0
31	1.1866281249851672E-4	1.1866281249851672E-4	0.0	1.0718811852991015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	860	0.0	29.686047	1
TAGACAG	60	1.3367007E-6	24.666666	5
GACGTAT	40	0.0019307618	23.125	28
TATACAC	285	0.0	22.719297	37
CTTATAC	1025	0.0	20.756098	37
ATACTTA	45	0.003824974	20.555555	6
ACGTATA	45	0.003824974	20.555555	29
TTTTACG	65	6.899335E-5	19.923077	4
GCGGTAA	480	0.0	19.65625	23
CGCGGTA	505	0.0	18.683168	22
GGACGTA	60	9.234031E-4	18.5	27
TCTATAA	50	0.0070333635	18.499998	2
GGTAATA	565	0.0	18.00885	25
CGGTAAT	535	0.0	17.635515	24
ATACGGC	105	4.79351E-7	17.61905	29
GTATCAA	1455	0.0	17.546392	2
GCCTAGT	95	3.6040765E-6	17.526316	1
TCACGTA	95	3.6040765E-6	17.526316	25
AGACAGT	85	2.7217196E-5	17.411764	6
TCTTATA	1945	0.0	17.311056	37
>>END_MODULE