FastQCFastQC Report
Fri 10 Feb 2017
ERR1631884.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631884.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194918
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5830.2991001344154978No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5400.27703957561641307No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5120.2626745605844509No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4520.23189238551596056No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4510.23137934926481907No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3710.1903364491734986No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3580.18366697790865905No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3270.16776285412327235No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3240.16622374536984783No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3120.16006731035614977No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3010.1544239115935932No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2820.1446762228219046No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2650.135954606552499No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2630.134928534050216No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2540.13031120778994246No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2330.11953744651597081No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2260.11594619275798028No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2230.11440708400455576No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2200.11286797525113124No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2190.11235493899998973No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2120.1087636852419992No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2110.1082506489908577No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2110.1082506489908577No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2050.10517243148400868No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2020.10363332273058415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2800.032.3751
GGACCGT401.5910573E-632.3756
TATCAGC250.005485289729.5999986
TACATAT250.005485289729.5999982
AGAAACG250.005485289729.59999820
TCAGCGT250.005485289729.5999988
CGGTCAA250.005485289729.59999810
TAGGACC250.005485289729.5999984
ATATTAA250.005485289729.5999981
GAAATGA358.842883E-426.42857230
TACGGCA358.842883E-426.42857230
GTGCTAG358.842883E-426.4285721
TAAGGAG358.842883E-426.4285724
CAGCGTG400.001925469323.1250029
GGGCTAA400.001925469323.1250021
ACCATTT400.001925469323.12500236
GTGTCGG400.001925469323.12500225
AAGGAGG400.001925469323.1250025
GGGTACT652.6639136E-622.769231
AGAAATG502.690962E-422.19999929
Produced by FastQC (version 0.11.5)