FastQCFastQC Report
Fri 10 Feb 2017
ERR1631883.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631883.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308398
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6820.22114280896763272No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5930.1922839966536748No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5380.17444989915628506No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4970.16115539011277635No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4840.15694005797702967No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4550.14753662475113327No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4440.14396980525165531No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4260.13813319152523687No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4230.1371604225708338No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4100.13294509043508712No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4080.1322965777988184No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3110.10084371493978561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATTA251.2315316E-437.015
GGTATCA3550.030.2253531
CTTAAAC250.005490185729.63
TTAAACC250.005490185729.64
ATACATT250.005490185729.66
TTAGCTA405.9262577E-527.754
TACGGCT405.9262577E-527.7530
ATACACA358.854625E-426.4285737
CTTATAC2350.023.61702237
TAATACG1650.023.54545227
CGTGCCA1650.023.54545210
GTAATAC1750.023.25714326
ATACGGC400.001928001523.12529
CGGTAAT1600.023.12524
AATACGG1700.022.85294228
TCCGTGC1650.022.424248
AAAAATA502.6958936E-422.230
TAACTCC1750.022.24
AACTCCG1850.022.05
CTCCGTG1800.021.5833327
Produced by FastQC (version 0.11.5)