##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631883.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	308398
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.619793254171555	34.0	31.0	34.0	31.0	34.0
2	32.79990466864247	34.0	31.0	34.0	31.0	34.0
3	32.88794998670549	34.0	31.0	34.0	31.0	34.0
4	36.28634426941809	37.0	37.0	37.0	35.0	37.0
5	36.20720627241422	37.0	35.0	37.0	35.0	37.0
6	36.26326694725647	37.0	37.0	37.0	35.0	37.0
7	36.251914733558586	37.0	37.0	37.0	35.0	37.0
8	36.23466753999702	37.0	37.0	37.0	35.0	37.0
9	38.07347648168925	39.0	38.0	39.0	37.0	39.0
10	38.00210118094151	39.0	38.0	39.0	35.0	39.0
11	38.075636028768024	39.0	38.0	39.0	37.0	39.0
12	38.00910187485003	39.0	38.0	39.0	35.0	39.0
13	38.06448809655056	39.0	38.0	39.0	37.0	39.0
14	39.47952969863618	41.0	39.0	41.0	37.0	41.0
15	39.477785199644615	41.0	39.0	41.0	37.0	41.0
16	39.43452616424231	41.0	39.0	41.0	37.0	41.0
17	39.39296298938385	40.0	39.0	41.0	37.0	41.0
18	39.408952068431056	41.0	39.0	41.0	37.0	41.0
19	39.425181097153676	41.0	39.0	41.0	37.0	41.0
20	39.41753189060889	41.0	39.0	41.0	37.0	41.0
21	39.376056264956325	40.0	39.0	41.0	37.0	41.0
22	39.366626891224975	40.0	39.0	41.0	36.0	41.0
23	39.29557584679537	40.0	39.0	41.0	36.0	41.0
24	39.31098126446994	40.0	39.0	41.0	36.0	41.0
25	39.2426831561813	40.0	39.0	41.0	36.0	41.0
26	39.190072568564	40.0	39.0	41.0	36.0	41.0
27	39.09174184008975	40.0	39.0	41.0	36.0	41.0
28	39.075739790789825	40.0	39.0	41.0	35.0	41.0
29	39.03832061167712	40.0	39.0	41.0	35.0	41.0
30	39.00100519458621	40.0	39.0	41.0	35.0	41.0
31	38.91856626826374	40.0	38.0	41.0	35.0	41.0
32	38.87707118723208	40.0	38.0	41.0	35.0	41.0
33	38.82023229722631	40.0	38.0	41.0	35.0	41.0
34	38.78853948469186	40.0	38.0	41.0	35.0	41.0
35	38.716253023690165	40.0	38.0	41.0	35.0	41.0
36	38.67267945965927	40.0	38.0	41.0	35.0	41.0
37	38.62322064345424	40.0	38.0	41.0	35.0	41.0
38	38.57024364619745	40.0	38.0	41.0	35.0	41.0
39	38.51151109929377	40.0	38.0	41.0	35.0	41.0
40	38.18545191603059	40.0	38.0	41.0	34.0	41.0
41	38.26386033631865	40.0	37.0	41.0	34.0	41.0
42	38.254622273815	40.0	37.0	41.0	34.0	41.0
43	37.78370806555166	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	9.0
20	15.0
21	26.0
22	52.0
23	91.0
24	131.0
25	241.0
26	429.0
27	656.0
28	1001.0
29	1479.0
30	2062.0
31	2899.0
32	3672.0
33	5141.0
34	7622.0
35	11552.0
36	18110.0
37	31730.0
38	72812.0
39	148663.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.96331364016628	16.25723902230235	12.594763908974766	28.184683428556607
2	20.520236836814764	19.384042698071973	32.317005946860874	27.77871451825239
3	21.07698493505146	19.698247070344166	28.84389652332376	30.380871471280617
4	15.866510158950447	15.543550866088626	33.00183529076064	35.588103684200284
5	17.044857619050706	32.61013365845434	33.43406896283374	16.91093975966122
6	35.39841373809169	32.85948676709965	15.161901179644484	16.58019831516417
7	31.127633771944048	27.382797553810335	19.93592695153665	21.553641722708967
8	27.864966698876124	30.769979053041848	19.97678324762158	21.388271000460442
9	28.407123262796773	12.913183613382706	17.46995765212485	41.209735471695666
10	19.04973443407545	23.81241123483291	30.28359457584031	26.85425975525133
11	38.99084948670225	19.254340170818228	18.803948144929606	22.95086219754992
12	23.065000421533213	23.615263393407222	26.492065447895254	26.827670737164315
13	34.27097451993852	17.580853312926802	21.34190234696723	26.806269820167444
14	24.49497078450574	19.48067108087601	22.862664479017376	33.16169365560088
15	29.296558343439322	24.041011939117634	19.869454406319107	26.79297531112394
16	26.448939357583384	23.847106660873287	21.897677676249522	27.80627630529381
17	27.24012477383122	23.585107555820723	21.667131434055992	27.507636236292065
18	26.814376228120807	21.926860744881612	23.374340949033392	27.88442207796419
19	28.176901276921377	22.158379756029547	23.181408439743446	26.483310527305626
20	29.387674368835075	21.09190072568564	22.862664479017376	26.657760426461905
21	27.40744103398855	22.47939351098256	21.93302161492617	28.180143840102723
22	28.496293750283723	22.75760543194184	21.168100960447216	27.577999857327217
23	27.848753882969408	22.276733312148586	22.516034474931747	27.35847832995026
24	27.97067425858793	21.92361818170027	22.693402680951237	27.412304878760562
25	27.95219164845427	21.901244495749	22.996582338406863	27.149981517389865
26	27.906471507597324	22.771872709939753	22.32893857936822	26.992717203094703
27	27.501151109929378	21.793267141810258	23.2290741185092	27.47650762975117
28	26.4641794045357	22.56045759051615	22.423297167945318	28.552065837002832
29	27.295572604232195	23.152873883747624	22.498848890070622	27.05270462194956
30	27.103937120214788	22.258574958333064	23.23426221959935	27.4032257018528
31	27.76704129079955	22.671677507636236	22.682053709816536	26.879227491747677
32	25.97617364574349	22.22841912074657	22.981018035136415	28.81438919837353
33	26.219041628026122	22.059481578998565	24.065655419295844	27.65582137367947
34	26.74466111972192	21.795536936037198	23.725510541572902	27.73429140266798
35	26.23719998184165	23.126284865660608	24.21189501877444	26.4246201337233
36	26.547189021978095	22.266032853650152	24.277394795037583	26.909383329334176
37	26.69278010882042	22.15740698707514	24.038742144890694	27.111070759213746
38	26.18013086985	21.676859123600025	24.970330546890708	27.17267945965927
39	25.773189190591378	20.894428627941817	25.739466533505407	27.5929156479614
40	25.363004948151413	21.868170351299295	26.31567001083016	26.453154689719128
41	23.978754726035838	21.001433212926155	27.36950304476683	27.65030901627118
42	22.987178905180965	21.364276032918504	27.83221681074456	27.816328251155976
43	22.63017269891504	21.642812210195917	27.868533518375603	27.858481572513437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	8.5
4	15.0
5	15.0
6	17.5
7	20.0
8	25.0
9	30.0
10	34.5
11	39.0
12	39.0
13	104.0
14	169.0
15	292.0
16	415.0
17	422.0
18	429.0
19	429.0
20	510.0
21	591.0
22	531.0
23	471.0
24	536.0
25	601.0
26	601.0
27	781.0
28	961.0
29	1339.5
30	1718.0
31	2121.0
32	2524.0
33	2524.0
34	3289.0
35	4054.0
36	4675.5
37	5297.0
38	6641.5
39	7986.0
40	7986.0
41	9367.0
42	10748.0
43	12154.0
44	13560.0
45	17474.0
46	21388.0
47	21388.0
48	23572.0
49	25756.0
50	29346.0
51	32936.0
52	34070.0
53	35204.0
54	35204.0
55	32336.5
56	29469.0
57	27947.5
58	26426.0
59	24371.5
60	22317.0
61	22317.0
62	20712.0
63	19107.0
64	16444.5
65	13782.0
66	12091.5
67	10401.0
68	10401.0
69	8895.0
70	7389.0
71	6438.0
72	5487.0
73	4482.0
74	3477.0
75	3477.0
76	2828.0
77	2179.0
78	1865.5
79	1552.0
80	1183.5
81	815.0
82	815.0
83	670.0
84	525.0
85	403.0
86	281.0
87	222.5
88	164.0
89	164.0
90	117.0
91	70.0
92	52.5
93	35.0
94	20.5
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	308398.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.91017833818593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.4171421622673	51.497594345937195
2	6.156119404762679	7.253161840114728
3	1.9897629981483669	3.5165187921493137
4	1.01924198119147	2.4017490752702177
5	0.6309190664866013	1.8583777361843734
6	0.4416924865181463	1.561210989085247
7	0.30774231086014214	1.2690408090483498
8	0.24067014661188468	1.1342337006066785
9	0.19873658156161178	1.0536846715904384
>10	1.404540166939531	16.411051996299094
>50	0.13761354562404599	5.559858687207968
>100	0.054161154502636574	5.893248351417194
>500	0.0016579945255909155	0.590269005089187
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	682	0.22114280896763272	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	593	0.1922839966536748	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	538	0.17444989915628506	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	497	0.16115539011277635	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	484	0.15694005797702967	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	455	0.14753662475113327	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	444	0.14396980525165531	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	426	0.13813319152523687	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	423	0.1371604225708338	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	410	0.13294509043508712	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	408	0.1322965777988184	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	311	0.10084371493978561	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.2425631813435884E-4	0.0	0.0	0.0	0.0
9	3.2425631813435884E-4	0.0	0.0	0.0	0.0
10	3.2425631813435884E-4	0.0	0.0	0.0	0.0
11	3.2425631813435884E-4	0.0	0.0	0.0	0.0
12	3.2425631813435884E-4	0.0	0.0	0.0	0.0
13	3.2425631813435884E-4	0.0	0.0	0.0	0.0
14	3.2425631813435884E-4	0.0	0.0	0.0	0.0
15	6.485126362687177E-4	0.0	0.0	3.2425631813435884E-4	0.0
16	6.485126362687177E-4	0.0	0.0	0.0012970252725374354	0.0
17	6.485126362687177E-4	0.0	0.0	0.0012970252725374354	0.0
18	6.485126362687177E-4	0.0	0.0	0.0016212815906717943	0.0
19	6.485126362687177E-4	0.0	0.0	0.0016212815906717943	0.0
20	6.485126362687177E-4	0.0	0.0	0.0029183068632092296	0.0
21	9.727689544030766E-4	0.0	0.0	0.004215332135746665	0.0
22	9.727689544030766E-4	0.0	0.0	0.008106407953358971	0.0
23	9.727689544030766E-4	0.0	0.0	0.009403433225896407	0.0
24	9.727689544030766E-4	0.0	0.0	0.01134897113470256	0.0
25	9.727689544030766E-4	0.0	0.0	0.011673227452836919	0.0
26	9.727689544030766E-4	0.0	0.0	0.012645996407239995	0.0
27	9.727689544030766E-4	0.0	0.0	0.02821029967768922	0.0
28	9.727689544030766E-4	0.0	0.0	0.08949474380508304	0.0
29	9.727689544030766E-4	0.0	0.0	0.207848299924124	0.0
30	9.727689544030766E-4	0.0	0.0	0.33690231454159886	0.0
31	9.727689544030766E-4	0.0	0.0	0.7263341526209638	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTATTA	25	1.2315316E-4	37.0	15
GGTATCA	355	0.0	30.225353	1
CTTAAAC	25	0.0054901857	29.6	3
TTAAACC	25	0.0054901857	29.6	4
ATACATT	25	0.0054901857	29.6	6
TTAGCTA	40	5.9262577E-5	27.75	4
TACGGCT	40	5.9262577E-5	27.75	30
ATACACA	35	8.854625E-4	26.42857	37
CTTATAC	235	0.0	23.617022	37
TAATACG	165	0.0	23.545452	27
CGTGCCA	165	0.0	23.545452	10
GTAATAC	175	0.0	23.257143	26
ATACGGC	40	0.0019280015	23.125	29
CGGTAAT	160	0.0	23.125	24
AATACGG	170	0.0	22.852942	28
TCCGTGC	165	0.0	22.42424	8
AAAAATA	50	2.6958936E-4	22.2	30
TAACTCC	175	0.0	22.2	4
AACTCCG	185	0.0	22.0	5
CTCCGTG	180	0.0	21.583332	7
>>END_MODULE