FastQCFastQC Report
Fri 10 Feb 2017
ERR1631882.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631882.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85729
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1890.22046215399689717No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1580.184301694875713No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1390.16213883283369687No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1390.16213883283369687No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1170.13647657152188875No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1120.1306442394055687No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1110.1294777729823047No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1030.12014604159619265No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC980.11431370947987261No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA980.11431370947987261No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT930.1084813773635526No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA900.10498197809376056No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA870.10148257882396855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCC250.00546835829.59999837
GGTATCA905.456968E-1226.7222211
GGGGAAC358.8023156E-426.4285719
GCAGTCG358.8023156E-426.428579
CATCTAA358.8023156E-426.4285731
CTGAAAC358.8023156E-426.4285725
AACATCT358.8023156E-426.4285729
ACATCTA358.8023156E-426.4285730
GATTCCT358.8023156E-426.4285718
AGTCGGT358.8023156E-426.4285711
TATGGGC551.8761646E-523.5454544
AACGTCA400.001916719723.12500228
CGGGTAA400.001916719723.12500223
AACCGGG400.001916719723.12500214
ATCTAAG400.001916719723.12500232
ACTGAAA400.001916719723.12500224
GCGAACC400.001916719723.12500211
TAACGTC400.001916719723.12500227
GGATACC400.001916719723.1250021
AATGAGC400.001916719723.12500234
Produced by FastQC (version 0.11.5)