##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631882.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85729
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61435453580469	34.0	31.0	34.0	31.0	34.0
2	32.78146251560149	34.0	31.0	34.0	31.0	34.0
3	32.88195359796568	34.0	31.0	34.0	31.0	34.0
4	36.298393775735164	37.0	37.0	37.0	35.0	37.0
5	36.210127261486775	37.0	35.0	37.0	35.0	37.0
6	36.26028531768713	37.0	37.0	37.0	35.0	37.0
7	36.24810740822826	37.0	37.0	37.0	35.0	37.0
8	36.2309720164705	37.0	37.0	37.0	35.0	37.0
9	38.08175763160657	39.0	38.0	39.0	37.0	39.0
10	38.00176136429913	39.0	38.0	39.0	35.0	39.0
11	38.09909132265628	39.0	38.0	39.0	37.0	39.0
12	38.01337936987484	39.0	38.0	39.0	35.0	39.0
13	38.06852990236676	39.0	38.0	39.0	37.0	39.0
14	39.46728644915956	40.0	39.0	41.0	37.0	41.0
15	39.46711147919607	41.0	39.0	41.0	37.0	41.0
16	39.43548857446138	40.0	39.0	41.0	37.0	41.0
17	39.41016458841232	40.0	39.0	41.0	37.0	41.0
18	39.42152597137491	41.0	39.0	41.0	37.0	41.0
19	39.44057436806681	41.0	39.0	41.0	37.0	41.0
20	39.41855148199559	41.0	39.0	41.0	37.0	41.0
21	39.372394405627034	40.0	39.0	41.0	37.0	41.0
22	39.36985150882432	40.0	39.0	41.0	36.0	41.0
23	39.30019013402699	40.0	39.0	41.0	36.0	41.0
24	39.329841710506365	40.0	39.0	41.0	36.0	41.0
25	39.26601266782536	40.0	39.0	41.0	36.0	41.0
26	39.19897584248037	40.0	39.0	41.0	36.0	41.0
27	39.09085606970803	40.0	39.0	41.0	36.0	41.0
28	39.10360554771431	40.0	39.0	41.0	35.0	41.0
29	39.080801129139495	40.0	39.0	41.0	35.0	41.0
30	39.03728026688752	40.0	39.0	41.0	35.0	41.0
31	38.9523265172812	40.0	38.0	41.0	35.0	41.0
32	38.91214174899976	40.0	38.0	41.0	35.0	41.0
33	38.87228359131682	40.0	38.0	41.0	35.0	41.0
34	38.832623732925846	40.0	38.0	41.0	35.0	41.0
35	38.786000069987985	40.0	38.0	41.0	35.0	41.0
36	38.74182598653898	40.0	38.0	41.0	35.0	41.0
37	38.68939332081326	40.0	38.0	41.0	35.0	41.0
38	38.64405277094099	40.0	38.0	41.0	35.0	41.0
39	38.5754062219319	40.0	38.0	41.0	35.0	41.0
40	38.243908129104504	40.0	38.0	41.0	34.0	41.0
41	38.341646350709794	40.0	38.0	41.0	34.0	41.0
42	38.3488317838771	40.0	37.0	41.0	34.0	41.0
43	37.90341658015374	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	7.0
22	7.0
23	16.0
24	36.0
25	75.0
26	103.0
27	181.0
28	241.0
29	388.0
30	537.0
31	749.0
32	1078.0
33	1451.0
34	2097.0
35	3160.0
36	5084.0
37	8738.0
38	19842.0
39	41936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24557617608977	16.40051791109193	11.988941898307457	28.36496401451084
2	21.292678090261173	19.321349834945	31.046670321594792	28.339301753199038
3	21.492143848639316	19.173208599190474	28.55393157507961	30.7807159770906
4	15.940930140325909	15.068413255724433	33.14281048420021	35.84784611974944
5	17.5681508007792	32.67739038131787	32.69838677693663	17.0560720409663
6	36.42641346568839	32.9876704499061	14.591328488609456	15.994587595796055
7	31.295127669750027	27.099347945269397	19.187206196269642	22.418318188710938
8	28.41395560428793	30.031844533355105	19.56864071667697	21.985559145679993
9	28.212156913063257	12.336548892440131	17.238040802995485	42.21325339150113
10	19.15804453568804	23.491467298113825	29.847542838479395	27.50294532771874
11	40.233759871222105	18.608638850330696	18.419671289761926	22.737929988685277
12	23.493800230960353	23.287335674042623	26.19183706797	27.027027027027028
13	35.24128357965216	17.341856314665982	20.26735410421211	27.149506001469746
14	24.685928915536167	18.43250242041783	22.371659531780377	34.509909132265626
15	29.561758564779712	23.683934257952384	19.573306582370027	27.181000594897874
16	26.80306547376034	23.259340479884287	21.570297098998008	28.367296947357367
17	27.41779327882047	23.387651786443328	21.300843355224018	27.893711579512182
18	27.565934514574998	20.90774417058405	22.870907160937374	28.65541415390358
19	28.265814368533405	21.936567555902904	22.461477446371706	27.33614062919199
20	29.494103512230403	21.169032649395188	22.41365232301788	26.92321151535653
21	28.00919175541532	21.637952151547317	22.05788006392236	28.294976029115006
22	28.320638290426807	22.539630696730395	20.964901025323986	28.174829987518805
23	28.44545019771606	22.20368836683036	21.689276674170934	27.661584761282647
24	28.520104048804956	21.136371589543796	21.767429924529623	28.57609443712163
25	28.529435780191065	21.5574659683421	22.325000874849817	27.588097376617014
26	28.71023807579699	21.966895682907765	21.406991799741046	27.9158744415542
27	27.670916492668756	21.56913063257474	22.956059209835644	27.803893664920853
28	27.0083635642548	22.158196176323063	21.919070559553944	28.914369699868192
29	27.625424302161463	22.953726276989116	22.00772200772201	27.413127413127413
30	27.28481610656837	21.79892451795775	23.110032777706493	27.80622659776738
31	28.35796521597126	21.98439267925673	22.31683560988697	27.340806494885044
32	26.26882385190542	22.263178154416824	22.175693172672027	29.292304821005725
33	26.372639363575917	21.713772469059478	23.963886199535747	27.949701967828855
34	26.93604264601244	21.566797699728212	23.093702247780797	28.403457406478555
35	26.513781800790863	22.928064015677307	23.951055068879842	26.607099114651984
36	26.96987017228709	21.56213183403516	24.221675279077093	27.246322714600662
37	27.11451200877183	21.777928122339	23.757421642618016	27.35013822627116
38	26.13118081396027	21.09437879830629	25.07669516732961	27.69774522040383
39	25.6634277782314	20.490149191055533	26.035530567252625	27.81089246346044
40	25.552613468021324	21.45015105740181	26.406466889850577	26.590768584726288
41	24.103862170327428	20.216029581588494	27.471450734290613	28.20865751379347
42	22.971223273338076	20.885581308542033	28.189994051021237	27.953201367098647
43	22.50347023760921	20.969566891017042	28.178329386788604	28.348633484585147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	4.0
9	5.0
10	6.5
11	8.0
12	8.0
13	17.0
14	26.0
15	44.5
16	63.0
17	59.5
18	56.0
19	56.0
20	78.0
21	100.0
22	83.5
23	67.0
24	85.0
25	103.0
26	103.0
27	142.5
28	182.0
29	234.0
30	286.0
31	383.0
32	480.0
33	480.0
34	660.0
35	840.0
36	1030.0
37	1220.0
38	1568.0
39	1916.0
40	1916.0
41	2311.0
42	2706.0
43	3179.0
44	3652.0
45	4714.5
46	5777.0
47	5777.0
48	6470.5
49	7164.0
50	8074.5
51	8985.0
52	9051.0
53	9117.0
54	9117.0
55	8746.0
56	8375.0
57	8029.5
58	7684.0
59	7347.0
60	7010.0
61	7010.0
62	6615.0
63	6220.0
64	5326.5
65	4433.0
66	3825.5
67	3218.0
68	3218.0
69	2714.5
70	2211.0
71	1866.0
72	1521.0
73	1211.5
74	902.0
75	902.0
76	727.0
77	552.0
78	446.0
79	340.0
80	270.0
81	200.0
82	200.0
83	176.0
84	152.0
85	112.5
86	73.0
87	58.5
88	44.0
89	44.0
90	35.0
91	26.0
92	17.0
93	8.0
94	5.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	85729.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.90083868935832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.88176339134557	60.551272031634575
2	6.008329383083903	8.279578672327917
3	2.0823457709758246	4.304261101844183
4	1.0513306697365747	2.8975025953877918
5	0.655177084038735	2.2571125290158522
6	0.4892666079772466	2.022652777939787
7	0.33859280828875193	1.633052992569609
8	0.22855014559490755	1.2597837371251268
9	0.17098936818581975	1.0603179787469819
>10	1.0310151012392497	12.256062709234914
>50	0.04909595720186904	2.232616734127308
>100	0.013543712331550077	1.245786140045959
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	189	0.22046215399689717	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	158	0.184301694875713	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	139	0.16213883283369687	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	139	0.16213883283369687	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	117	0.13647657152188875	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	112	0.1306442394055687	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	111	0.1294777729823047	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	103	0.12014604159619265	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	98	0.11431370947987261	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	98	0.11431370947987261	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	93	0.1084813773635526	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	90	0.10498197809376056	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	87	0.10148257882396855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0011664664232640064	0.0
20	0.0	0.0	0.0	0.002332932846528013	0.0
21	0.0	0.0	0.0	0.002332932846528013	0.0
22	0.0	0.0	0.0	0.003499399269792019	0.0
23	0.0	0.0	0.0	0.004665865693056026	0.0
24	0.0	0.0	0.0	0.004665865693056026	0.0
25	0.0	0.0	0.0	0.005832332116320032	0.0
26	0.0	0.0	0.0	0.006998798539584038	0.0
27	0.0	0.0	0.0	0.02799519415833615	0.0
28	0.0	0.0	0.0	0.09448378028438452	0.0
29	0.0	0.0	0.0	0.20646455691772914	0.0
30	0.0	0.0	0.0	0.3487734605559379	0.0
31	0.0	0.0	0.0	0.738373245926116	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACCC	25	0.005468358	29.599998	37
GGTATCA	90	5.456968E-12	26.722221	1
GGGGAAC	35	8.8023156E-4	26.42857	19
GCAGTCG	35	8.8023156E-4	26.42857	9
CATCTAA	35	8.8023156E-4	26.42857	31
CTGAAAC	35	8.8023156E-4	26.42857	25
AACATCT	35	8.8023156E-4	26.42857	29
ACATCTA	35	8.8023156E-4	26.42857	30
GATTCCT	35	8.8023156E-4	26.42857	18
AGTCGGT	35	8.8023156E-4	26.42857	11
TATGGGC	55	1.8761646E-5	23.545454	4
AACGTCA	40	0.0019167197	23.125002	28
CGGGTAA	40	0.0019167197	23.125002	23
AACCGGG	40	0.0019167197	23.125002	14
ATCTAAG	40	0.0019167197	23.125002	32
ACTGAAA	40	0.0019167197	23.125002	24
GCGAACC	40	0.0019167197	23.125002	11
TAACGTC	40	0.0019167197	23.125002	27
GGATACC	40	0.0019167197	23.125002	1
AATGAGC	40	0.0019167197	23.125002	34
>>END_MODULE