FastQCFastQC Report
Fri 10 Feb 2017
ERR1631881.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631881.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496374
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC12200.2457824140668125No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9530.1919923283653052No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT9400.1893733354285277No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9060.1825236615938788No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8280.16680970397321374No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7790.1569381152115139No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7750.15613227123096698No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7750.15613227123096698No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7300.14706652644981405No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6920.13941100863461825No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6290.12671896594100418No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6110.123092668028543No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG6050.1218839020577226No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5780.11644445518903086No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG5320.1071772494127412No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT5230.10536410045651062No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT5130.10334949050514329No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5110.10294656851486984No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC5090.10254364652459637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAAA200.00184092937.01
CTAAACA250.00549337229.64
GGTATCA6400.027.751
GTCTCGC551.8998364E-523.5454541
CATACTG400.001929650323.1255
ATACGGC652.6777489E-622.7692329
GCGGTAA3850.022.10389523
TCGGTAG603.720798E-521.58333228
AACTCCG4150.020.9518075
TCCAGGA3800.020.9342122
CTAACTC4200.020.7023813
TCCGTGC4200.020.7023818
GTACCGG450.003822791220.5555576
TGTCTAA450.003822791220.55555715
TACTACT450.003822791220.5555574
CAGTCGG909.451105E-820.55555710
GCTAACT4150.020.5060232
TAACTCC4150.020.5060234
GTATCAA8700.020.4137942
CTCCGTG4200.020.2619047
Produced by FastQC (version 0.11.5)