##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631881.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	496374
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.580294294221694	34.0	31.0	34.0	31.0	34.0
2	32.7554888048125	34.0	31.0	34.0	31.0	34.0
3	32.84484279998549	34.0	31.0	34.0	31.0	34.0
4	36.267755764806374	37.0	37.0	37.0	35.0	37.0
5	36.192816303835414	37.0	35.0	37.0	35.0	37.0
6	36.24043966847579	37.0	37.0	37.0	35.0	37.0
7	36.22870255089912	37.0	37.0	37.0	35.0	37.0
8	36.214717128616726	37.0	37.0	37.0	35.0	37.0
9	38.04773215357775	39.0	38.0	39.0	35.0	39.0
10	37.97976525764847	39.0	38.0	39.0	35.0	39.0
11	38.05387268470951	39.0	38.0	39.0	35.0	39.0
12	37.99009819208903	39.0	38.0	39.0	35.0	39.0
13	38.035015935564715	39.0	38.0	39.0	35.0	39.0
14	39.43269591074472	40.0	39.0	41.0	37.0	41.0
15	39.43246423060032	40.0	39.0	41.0	37.0	41.0
16	39.39531683770705	40.0	39.0	41.0	37.0	41.0
17	39.34261262676933	40.0	39.0	41.0	36.0	41.0
18	39.338621684455674	40.0	39.0	41.0	36.0	41.0
19	39.368458057835426	40.0	39.0	41.0	36.0	41.0
20	39.335533287400224	40.0	39.0	41.0	36.0	41.0
21	39.314271093973495	40.0	39.0	41.0	36.0	41.0
22	39.290444302078676	40.0	39.0	41.0	36.0	41.0
23	39.232236176753815	40.0	39.0	41.0	36.0	41.0
24	39.24764592827183	40.0	39.0	41.0	36.0	41.0
25	39.178456164102066	40.0	39.0	41.0	36.0	41.0
26	39.113470890900814	40.0	39.0	41.0	36.0	41.0
27	39.026723801004884	40.0	39.0	41.0	35.0	41.0
28	39.00049760865799	40.0	39.0	41.0	35.0	41.0
29	38.98144342773796	40.0	39.0	41.0	35.0	41.0
30	38.94085306643781	40.0	38.0	41.0	35.0	41.0
31	38.87571468288025	40.0	38.0	41.0	35.0	41.0
32	38.825143943881024	40.0	38.0	41.0	35.0	41.0
33	38.767485807072894	40.0	38.0	41.0	35.0	41.0
34	38.72356529552314	40.0	38.0	41.0	35.0	41.0
35	38.63783558365265	40.0	38.0	41.0	35.0	41.0
36	38.6193535519588	40.0	38.0	41.0	35.0	41.0
37	38.576152256161684	40.0	38.0	41.0	35.0	41.0
38	38.542923279623835	40.0	38.0	41.0	34.0	41.0
39	38.475665526397435	40.0	38.0	41.0	34.0	41.0
40	38.13908665643245	40.0	37.0	41.0	34.0	41.0
41	38.21845825929642	40.0	37.0	41.0	34.0	41.0
42	38.216735767788	40.0	37.0	41.0	34.0	41.0
43	37.736970510139535	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	4.0
19	8.0
20	18.0
21	35.0
22	76.0
23	127.0
24	233.0
25	400.0
26	678.0
27	1021.0
28	1537.0
29	2280.0
30	3363.0
31	4714.0
32	6308.0
33	8898.0
34	13030.0
35	20222.0
36	30375.0
37	51737.0
38	119726.0
39	231580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.39751880638389	16.22385539935613	11.974640089932189	28.403985704327784
2	21.028297211376906	18.37707051537752	32.892939597964435	27.701692675281137
3	21.127617481979314	19.93940053266287	28.58812105388276	30.34486093147506
4	15.505646951693683	15.041883740888926	33.91998775117149	35.53248155624589
5	16.781902355884878	32.98299266279056	33.561588640823246	16.673516340501315
6	35.635428124760764	33.57387776152659	14.638357367630054	16.15233674608259
7	31.245593040731386	27.2764085145475	19.94786189445861	21.530136550262505
8	28.010532380825747	30.693992836047013	19.52036166277847	21.77511312034877
9	28.698521679217688	12.512742407942397	17.18905502705621	41.599680885783705
10	19.71678613303678	22.911353132919935	29.773719010262422	27.598141723780863
11	39.440220478913076	19.309834922860585	18.724993653978654	22.524950944247685
12	24.415460922610773	23.216566540552083	25.753564852308948	26.614407684528196
13	33.63351021608707	17.478755938062832	21.003114586984815	27.88461925886529
14	24.082042975659483	19.18372033990499	23.08964611361594	33.64459057081958
15	29.735441421186444	23.270155165258455	20.434188736718685	26.56021467683642
16	26.500783683271084	23.454894897798837	21.33492084597501	28.70940057295507
17	26.762078593963423	23.815711540088724	21.720315729671576	27.701894136276277
18	26.275147368717946	21.47594354257072	23.608408176093025	28.640500912618304
19	28.15296530438742	21.770882439450897	22.827948280933327	27.248203975228357
20	28.671122983879094	20.921724344949574	23.14061574538554	27.2665369257858
21	28.612497834294302	21.69392433930867	21.677203076712317	28.016374749684715
22	29.12279853497564	22.872068238868273	20.782514797310093	27.22261842884599
23	28.367722725203176	22.480025142332195	21.772494127411992	27.37975800505264
24	28.747879623026186	21.840789404763346	21.50938606776342	27.901944904447053
25	27.954727685172877	21.68747758746429	22.95849500578193	27.399299721580906
26	28.587919592887623	22.195360756203993	22.5217275683255	26.694992082582893
27	28.48396571939707	21.40402196730691	22.62890481773824	27.483107495557785
28	26.47701128584495	22.088384967786386	22.742528818995357	28.692074927373312
29	28.07701450922087	22.52756993718446	22.753810634723013	26.641604918871657
30	26.930499985897733	21.107068460475368	23.990579683867406	27.971851869759494
31	27.323348926414358	22.350284261464136	22.90309323211933	27.423273580002178
32	25.698364539641478	22.04305624388062	23.076551148932055	29.18202806754584
33	26.709497274232735	22.030565662182145	24.13341552941935	27.12652153416577
34	27.004839093103183	21.92620886670132	23.806242873317295	27.262709166878203
35	26.590635287102067	23.33845044260981	23.885215583410897	26.185698686877235
36	26.98227546164787	22.235451494236198	23.512109820417667	27.27016322369826
37	26.080737508410994	22.301127778650777	24.115284039857045	27.502850673081188
38	26.741529572459477	21.173550588870487	24.885670885259906	27.199248953410134
39	25.178796633183847	21.031520587299095	25.83334340638309	27.956339373133964
40	25.414505997493826	21.456603287037595	26.825740268426628	26.30315044704195
41	23.77642664603706	20.384629331915047	27.57134741142767	28.267596610620217
42	22.43147304250424	21.62844951588923	27.90758581231088	28.032491629295652
43	22.41052109901002	20.632023433942955	28.394517037556362	28.562938429490664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	5.5
2	5.0
3	9.5
4	14.0
5	14.0
6	20.5
7	27.0
8	27.0
9	27.0
10	40.0
11	53.0
12	53.0
13	126.5
14	200.0
15	378.5
16	557.0
17	518.5
18	480.0
19	480.0
20	570.0
21	660.0
22	557.0
23	454.0
24	503.5
25	553.0
26	553.0
27	723.5
28	894.0
29	1324.0
30	1754.0
31	2433.0
32	3112.0
33	3112.0
34	4781.0
35	6450.0
36	7821.0
37	9192.0
38	11830.0
39	14468.0
40	14468.0
41	16547.5
42	18627.0
43	22537.5
44	26448.0
45	30269.0
46	34090.0
47	34090.0
48	37050.5
49	40011.0
50	45685.5
51	51360.0
52	52263.0
53	53166.0
54	53166.0
55	50700.0
56	48234.0
57	44400.0
58	40566.0
59	37615.5
60	34665.0
61	34665.0
62	33343.0
63	32021.0
64	25763.0
65	19505.0
66	17078.5
67	14652.0
68	14652.0
69	12757.0
70	10862.0
71	10183.5
72	9505.0
73	10308.5
74	11112.0
75	11112.0
76	9488.5
77	7865.0
78	5279.5
79	2694.0
80	1804.5
81	915.0
82	915.0
83	725.5
84	536.0
85	414.5
86	293.0
87	242.0
88	191.0
89	191.0
90	145.5
91	100.0
92	68.0
93	36.0
94	24.5
95	13.0
96	13.0
97	7.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	496374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.53773839766104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.91945844091546	34.019050527512185
2	6.858038372518063	5.560187309325166
3	2.4712997062944275	3.0054270298794017
4	1.3843942343847422	2.244808452508757
5	0.8765614508295259	1.7766909391600767
6	0.598978615840064	1.4568743060830573
7	0.46414774959109917	1.3170850035550943
8	0.3411119030638443	1.1062324072584344
9	0.2862213391898559	1.0442489194715925
>10	2.2425198945639253	18.12573917855622
>50	0.3026888928566708	8.64367068837289
>100	0.2450577294816392	18.935302658687895
>500	0.009020529919569541	2.516838303991978
>1k	5.011405510871967E-4	0.2478442756372761
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1220	0.2457824140668125	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	953	0.1919923283653052	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	940	0.1893733354285277	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	906	0.1825236615938788	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	828	0.16680970397321374	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	779	0.1569381152115139	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	775	0.15613227123096698	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	775	0.15613227123096698	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	730	0.14706652644981405	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	692	0.13941100863461825	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	629	0.12671896594100418	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	611	0.123092668028543	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	605	0.1218839020577226	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	578	0.11644445518903086	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	532	0.1071772494127412	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	523	0.10536410045651062	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	513	0.10334949050514329	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	511	0.10294656851486984	No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC	509	0.10254364652459637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.0146099513673158E-4	0.0	0.0	0.0	0.0
8	4.0292199027346315E-4	0.0	0.0	0.0	2.0146099513673158E-4
9	4.0292199027346315E-4	0.0	0.0	0.0	2.0146099513673158E-4
10	8.058439805469263E-4	0.0	0.0	0.0	2.0146099513673158E-4
11	8.058439805469263E-4	0.0	0.0	0.0	2.0146099513673158E-4
12	8.058439805469263E-4	0.0	0.0	0.0	2.0146099513673158E-4
13	8.058439805469263E-4	0.0	0.0	0.0	2.0146099513673158E-4
14	8.058439805469263E-4	0.0	0.0	0.0	2.0146099513673158E-4
15	0.0010073049756836579	0.0	0.0	4.0292199027346315E-4	2.0146099513673158E-4
16	0.0010073049756836579	0.0	0.0	6.043829854101947E-4	2.0146099513673158E-4
17	0.0010073049756836579	0.0	0.0	0.0012087659708203895	2.0146099513673158E-4
18	0.0010073049756836579	0.0	0.0	0.0012087659708203895	2.0146099513673158E-4
19	0.0010073049756836579	0.0	0.0	0.0020146099513673158	2.0146099513673158E-4
20	0.0010073049756836579	0.0	0.0	0.002417531941640779	2.0146099513673158E-4
21	0.0010073049756836579	0.0	0.0	0.0046336028881448265	2.0146099513673158E-4
22	0.0010073049756836579	0.0	0.0	0.007252595824922337	2.0146099513673158E-4
23	0.0010073049756836579	0.0	0.0	0.008662822790879458	2.0146099513673158E-4
24	0.0010073049756836579	0.0	0.0	0.0114832767227937	2.0146099513673158E-4
25	0.0010073049756836579	0.0	0.0	0.012087659708203895	2.0146099513673158E-4
26	0.0012087659708203895	0.0	0.0	0.015512496625528331	2.0146099513673158E-4
27	0.0012087659708203895	0.0	0.0	0.03364398618783417	2.0146099513673158E-4
28	0.001410226965957121	0.0	0.0	0.11463130623280027	4.0292199027346315E-4
29	0.001410226965957121	0.0	0.0	0.23067283943155767	4.0292199027346315E-4
30	0.001410226965957121	0.0	0.0	0.36968092607590247	4.0292199027346315E-4
31	0.001410226965957121	0.0	0.0	0.7564860367384271	4.0292199027346315E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTAAA	20	0.001840929	37.0	1
CTAAACA	25	0.005493372	29.6	4
GGTATCA	640	0.0	27.75	1
GTCTCGC	55	1.8998364E-5	23.545454	1
CATACTG	40	0.0019296503	23.125	5
ATACGGC	65	2.6777489E-6	22.76923	29
GCGGTAA	385	0.0	22.103895	23
TCGGTAG	60	3.720798E-5	21.583332	28
AACTCCG	415	0.0	20.951807	5
TCCAGGA	380	0.0	20.934212	2
CTAACTC	420	0.0	20.702381	3
TCCGTGC	420	0.0	20.702381	8
GTACCGG	45	0.0038227912	20.555557	6
TGTCTAA	45	0.0038227912	20.555557	15
TACTACT	45	0.0038227912	20.555557	4
CAGTCGG	90	9.451105E-8	20.555557	10
GCTAACT	415	0.0	20.506023	2
TAACTCC	415	0.0	20.506023	4
GTATCAA	870	0.0	20.413794	2
CTCCGTG	420	0.0	20.261904	7
>>END_MODULE