FastQCFastQC Report
Fri 10 Feb 2017
ERR1631880.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631880.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46908
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1640.34962053381086383No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1500.31977487848554614No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1490.3176430459623092No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1380.2941928882067025No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1320.28140189306728064No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1190.25368807026519996No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1130.2408970751257781No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1080.23023791250959325No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT960.20465592223074958No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA940.20039225718427559No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA930.19826042466103863No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC910.1939967596145647No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT730.15562377419629914No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA720.15349194167306215No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA680.14496461158011428No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA660.14070094653364032No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC650.13856911401040334No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG650.13856911401040334No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC620.13217361644069242No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT620.13217361644069242No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA620.13217361644069242No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA610.13004178391745544No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT600.12790995139421849No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA600.12790995139421849No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT600.12790995139421849No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT560.11938262130127059No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC560.11938262130127059No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC550.11725078877803359No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC540.11511895625479662No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC520.11085529120832267No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA520.11085529120832267No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG500.10659162616184871No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA490.10445979363861174No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC490.10445979363861174No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC490.10445979363861174No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA480.10232796111537479No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC470.10019612859213779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCCG700.034.3571435
TAACTCC700.034.3571434
GCTAACT700.034.3571432
GGTATCA507.01948E-933.3000031
CTAACTC750.032.0666663
GGCTAAC750.032.0666661
CCGTGCC801.8189894E-1230.06259
CGTGCCA801.8189894E-1230.062510
GGTAATA801.8189894E-1230.062525
CACAATA250.00544341929.634
AATATGT250.00544341929.637
TCACAAT250.00544341929.633
ACAATAT250.00544341929.635
TCCGTGC851.8189894E-1228.2941178
CTCCGTG851.8189894E-1228.2941177
GTATCAA604.14002E-827.7500022
GCGGTAA903.6379788E-1226.72222123
GCCAGCA903.6379788E-1226.72222113
CGGTAAT903.6379788E-1226.72222124
TTCCACT358.742659E-426.4285729
Produced by FastQC (version 0.11.5)