##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631880.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	46908
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57378272362923	34.0	31.0	34.0	31.0	34.0
2	32.73836019442312	34.0	31.0	34.0	31.0	34.0
3	32.82099002302379	34.0	31.0	34.0	31.0	34.0
4	36.25466871322589	37.0	37.0	37.0	35.0	37.0
5	36.16992837042722	37.0	35.0	37.0	35.0	37.0
6	36.23034450413576	37.0	36.0	37.0	35.0	37.0
7	36.21194678946022	37.0	37.0	37.0	35.0	37.0
8	36.19190756374179	37.0	36.0	37.0	35.0	37.0
9	38.045024302890766	39.0	38.0	39.0	35.0	39.0
10	37.95872772235013	39.0	38.0	39.0	35.0	39.0
11	38.04704954378784	39.0	38.0	39.0	35.0	39.0
12	37.9646968534152	39.0	38.0	39.0	35.0	39.0
13	38.00051163980557	39.0	38.0	39.0	35.0	39.0
14	39.38313294107615	40.0	39.0	41.0	37.0	41.0
15	39.40745715016628	40.0	39.0	41.0	37.0	41.0
16	39.33798072823399	40.0	39.0	41.0	36.0	41.0
17	39.32828089025326	40.0	39.0	41.0	36.0	41.0
18	39.34620960177369	40.0	39.0	41.0	36.0	41.0
19	39.339643557602116	40.0	39.0	41.0	36.0	41.0
20	39.35401210880873	40.0	39.0	41.0	36.0	41.0
21	39.31939114863136	40.0	39.0	41.0	36.0	41.0
22	39.277031636394646	40.0	39.0	41.0	36.0	41.0
23	39.220196981325145	40.0	39.0	41.0	36.0	41.0
24	39.23976720388846	40.0	39.0	41.0	36.0	41.0
25	39.16327705295472	40.0	39.0	41.0	36.0	41.0
26	39.114820499701544	40.0	39.0	41.0	36.0	41.0
27	39.00164151104289	40.0	39.0	41.0	35.0	41.0
28	39.004348938347405	40.0	39.0	41.0	35.0	41.0
29	38.96303402404707	40.0	39.0	41.0	35.0	41.0
30	38.941204059009124	40.0	38.0	41.0	35.0	41.0
31	38.84751001961286	40.0	38.0	41.0	35.0	41.0
32	38.81431738722606	40.0	38.0	41.0	35.0	41.0
33	38.74464910036667	40.0	38.0	41.0	35.0	41.0
34	38.733670162872	40.0	38.0	41.0	35.0	41.0
35	38.627931269719454	40.0	38.0	41.0	35.0	41.0
36	38.57740683891873	40.0	38.0	41.0	35.0	41.0
37	38.54751854694295	40.0	38.0	41.0	35.0	41.0
38	38.48320115971689	40.0	38.0	41.0	34.0	41.0
39	38.38441204059009	40.0	38.0	41.0	34.0	41.0
40	38.08527330092948	40.0	37.0	41.0	34.0	41.0
41	38.16754071800119	40.0	37.0	41.0	34.0	41.0
42	38.12938091583525	40.0	37.0	41.0	34.0	41.0
43	37.67380830561951	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	1.0
21	2.0
22	6.0
23	25.0
24	29.0
25	31.0
26	83.0
27	104.0
28	152.0
29	226.0
30	330.0
31	476.0
32	603.0
33	830.0
34	1231.0
35	1892.0
36	2843.0
37	5037.0
38	11278.0
39	21726.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.70273727295984	16.05696256502089	12.682271680736761	28.55802848128251
2	21.778801057388932	19.28668883772491	31.29743327364202	27.63707683124414
3	21.32685256246269	19.256843182399592	29.24661038628805	30.169693868849663
4	15.997271254370258	15.862965805406326	33.983542252920614	34.156220687302806
5	16.500383729854182	32.78545237486143	34.03044256843182	16.683721326852563
6	33.847104971433446	34.02404707086211	15.615673232710838	16.513174724993604
7	29.61541741280805	27.71808646712714	20.403769079901082	22.262727040163725
8	27.082800375202527	29.91600579858446	20.9409908757568	22.060202950456212
9	28.40453653960945	12.343310309542083	17.404280719706662	41.84787243114181
10	19.27816150763196	23.106932719365567	29.34254284983372	28.272362923168753
11	39.97399164321651	18.745203376822715	18.395582843011855	22.885222136948922
12	23.02592308348256	23.78059179670845	26.449646115801144	26.743839004007846
13	35.548307324976555	17.120746994116143	20.461328558028484	26.869617122878825
14	24.26025411443677	19.640573036582246	23.254029163468918	32.84514368551207
15	30.218726016884112	23.601517864756545	19.484949262385946	26.694806855973397
16	25.78451436855121	23.648418180267758	22.239276882408117	28.327790568772915
17	27.04442738978426	23.91702907819562	20.975100196128594	28.06344333589153
18	26.75023450157756	21.288479577044427	22.90014496461158	29.061140956766433
19	28.125266479065402	22.19664023194338	23.292402148887184	26.385691140104033
20	30.79858446320457	20.120235354310566	22.35865950370939	26.722520678775474
21	27.321565617805067	21.7020550865524	21.9557431568176	29.020636138824933
22	29.38091583525198	22.011170802421763	20.898354225292064	27.709559137034194
23	28.587874136607827	21.535772149739916	21.861942525795172	28.01441118785708
24	28.385350046900314	21.179756118359343	22.220090389698985	28.21480344504136
25	28.498337170631878	21.86620619084165	22.6592478894858	26.976208749040676
26	28.956681163127822	22.215826724652512	21.957874989340837	26.869617122878825
27	27.15741451351582	21.416389528438646	24.418009721156306	27.00818623688923
28	26.603138057474208	22.47804212501066	21.45689434638015	29.461925471134986
29	27.33862027799096	23.59938603223331	21.74682356954038	27.315170120235354
30	26.735311673914897	21.657286603564422	23.045109576191695	28.562292146328982
31	28.31073590858702	22.657116056962565	22.384241493988235	26.64790654046218
32	26.385691140104033	21.565617805065234	22.422614479406498	29.626076575424236
33	26.27057218384924	21.39720303572951	24.332736420226826	27.999488360194423
34	26.71399334868253	21.467553508996335	22.92359512236719	28.89485801995395
35	25.87405133452716	23.85094226997527	24.26025411443677	26.014752281060797
36	26.871748955402065	22.01969813251471	24.420141553679542	26.688411358403684
37	27.48998038714079	22.235013217361644	23.46294875074614	26.81205764475143
38	26.36437281487166	21.896051846166962	24.95096785196555	26.78860748699582
39	25.8761831670504	21.092350984906627	25.014922827662662	28.01654302038032
40	25.88471049714334	21.887524516074016	26.159716892640915	26.068048094141727
41	23.846678604928798	20.936727210710327	26.63511554532276	28.581478639038117
42	22.220090389698985	21.682868593843267	28.30434041101731	27.792700605440434
43	22.164662744094823	22.390636991557944	27.607231175918816	27.837469088428413
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	3.5
7	6.0
8	4.0
9	2.0
10	6.5
11	11.0
12	11.0
13	21.0
14	31.0
15	37.5
16	44.0
17	43.5
18	43.0
19	43.0
20	66.0
21	89.0
22	72.0
23	55.0
24	64.5
25	74.0
26	74.0
27	92.5
28	111.0
29	158.0
30	205.0
31	239.0
32	273.0
33	273.0
34	344.5
35	416.0
36	481.0
37	546.0
38	733.0
39	920.0
40	920.0
41	1117.0
42	1314.0
43	1534.5
44	1755.0
45	2574.0
46	3393.0
47	3393.0
48	3729.0
49	4065.0
50	4765.5
51	5466.0
52	5634.5
53	5803.0
54	5803.0
55	5271.0
56	4739.0
57	4561.5
58	4384.0
59	4108.5
60	3833.0
61	3833.0
62	3461.0
63	3089.0
64	2562.0
65	2035.0
66	1714.0
67	1393.0
68	1393.0
69	1195.0
70	997.0
71	860.0
72	723.0
73	571.0
74	419.0
75	419.0
76	338.0
77	257.0
78	219.0
79	181.0
80	144.0
81	107.0
82	107.0
83	76.5
84	46.0
85	40.5
86	35.0
87	31.0
88	27.0
89	27.0
90	22.0
91	17.0
92	10.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	46908.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.0167988402831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.6762129941057	55.48733691481197
2	6.024176629258384	7.712970069071373
3	2.3543907556029176	4.521616781785623
4	1.3986479736254953	3.5814786390381173
5	0.799227413500283	2.558199027884369
6	0.4528955343168271	1.7395753389613713
7	0.4262546205334843	1.9101219408203294
8	0.2963801658396883	1.5178647565447259
9	0.2530886809417563	1.4581734458940905
>10	1.2154916913650138	13.952843864585999
>50	0.07659262712711046	3.2723629231687554
>100	0.026640913783342768	2.2874562974332737
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	164	0.34962053381086383	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	150	0.31977487848554614	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	149	0.3176430459623092	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	138	0.2941928882067025	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	132	0.28140189306728064	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	119	0.25368807026519996	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	113	0.2408970751257781	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	108	0.23023791250959325	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	96	0.20465592223074958	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	94	0.20039225718427559	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	93	0.19826042466103863	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	91	0.1939967596145647	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	73	0.15562377419629914	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	72	0.15349194167306215	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	68	0.14496461158011428	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	66	0.14070094653364032	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	65	0.13856911401040334	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	65	0.13856911401040334	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	62	0.13217361644069242	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	62	0.13217361644069242	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	62	0.13217361644069242	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	61	0.13004178391745544	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	60	0.12790995139421849	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	60	0.12790995139421849	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	60	0.12790995139421849	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	56	0.11938262130127059	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	56	0.11938262130127059	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	55	0.11725078877803359	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	54	0.11511895625479662	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	52	0.11085529120832267	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	52	0.11085529120832267	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	50	0.10659162616184871	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	49	0.10445979363861174	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	49	0.10445979363861174	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	49	0.10445979363861174	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	48	0.10232796111537479	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	47	0.10019612859213779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.004263665046473949	0.0
25	0.0	0.0	0.0	0.006395497569710923	0.0
26	0.0	0.0	0.0	0.006395497569710923	0.0
27	0.0	0.0	0.0	0.036241152895028564	0.0
28	0.0	0.0	0.0	0.08527330092947898	0.0
29	0.0	0.0	0.0	0.1769420994286689	0.0
30	0.0	0.0	0.0	0.33256587362496803	0.0
31	0.0	0.0	0.0	0.6800545749125949	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACTCCG	70	0.0	34.357143	5
TAACTCC	70	0.0	34.357143	4
GCTAACT	70	0.0	34.357143	2
GGTATCA	50	7.01948E-9	33.300003	1
CTAACTC	75	0.0	32.066666	3
GGCTAAC	75	0.0	32.066666	1
CCGTGCC	80	1.8189894E-12	30.0625	9
CGTGCCA	80	1.8189894E-12	30.0625	10
GGTAATA	80	1.8189894E-12	30.0625	25
CACAATA	25	0.005443419	29.6	34
AATATGT	25	0.005443419	29.6	37
TCACAAT	25	0.005443419	29.6	33
ACAATAT	25	0.005443419	29.6	35
TCCGTGC	85	1.8189894E-12	28.294117	8
CTCCGTG	85	1.8189894E-12	28.294117	7
GTATCAA	60	4.14002E-8	27.750002	2
GCGGTAA	90	3.6379788E-12	26.722221	23
GCCAGCA	90	3.6379788E-12	26.722221	13
CGGTAAT	90	3.6379788E-12	26.722221	24
TTCCACT	35	8.742659E-4	26.42857	29
>>END_MODULE