FastQCFastQC Report
Fri 10 Feb 2017
ERR1631879.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631879.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823289
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22940.27863848539212843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.27135064357716426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17770.2158415817531875No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16060.19507123258053977No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA14840.1802526208901127No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14680.17830919640612228No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13240.16081837605020835No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12100.14697147660177654No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11550.1402909549380594No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11400.13846899448431838No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11040.1340962893953399No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10490.1274157677316228No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9940.12073524606790569No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9930.12061378203765628No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9560.11611961291842841No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8910.10822445095221728No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8870.10773859483121966No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8650.1050663861657328No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8480.10300149765149298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.027.2709181
ACCTCTA603.724634E-521.58333231
CGTTCGT450.00382489920.55555714
GTATGGA450.00382489920.5555571
GTCTATA555.141392E-420.1818182
GGCTTAT555.141392E-420.18181817
ATAACGC1403.6379788E-1219.82142835
TATACAC2450.019.63265437
CTTATAC8250.019.2848537
GCGGTAA6300.018.523
TATTAGA1306.9485395E-1018.52
GTATAGA609.233783E-418.51
TGTACAC609.233783E-418.55
GTACTGG3950.017.797471
GTATCAA19100.017.725132
ATACGGC1156.398295E-817.69565229
TACGGCT1054.7931826E-717.61904730
GTATTAG1950.017.0769211
TCTTATA15250.016.98360637
CGCGGTA7000.016.6522
Produced by FastQC (version 0.11.5)