##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631879.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	823289
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64240017782334	34.0	31.0	34.0	31.0	34.0
2	32.802155743608864	34.0	31.0	34.0	31.0	34.0
3	32.894167175803396	34.0	31.0	34.0	31.0	34.0
4	36.2972285552218	37.0	37.0	37.0	35.0	37.0
5	36.22580770543515	37.0	35.0	37.0	35.0	37.0
6	36.282440309538934	37.0	37.0	37.0	35.0	37.0
7	36.273329292629924	37.0	37.0	37.0	35.0	37.0
8	36.25793979999733	37.0	37.0	37.0	35.0	37.0
9	38.08979957220369	39.0	38.0	39.0	37.0	39.0
10	38.02069139755299	39.0	38.0	39.0	35.0	39.0
11	38.09715178995468	39.0	38.0	39.0	37.0	39.0
12	38.038244164564325	39.0	38.0	39.0	35.0	39.0
13	38.089755845152794	39.0	38.0	39.0	37.0	39.0
14	39.48477144720748	41.0	39.0	41.0	37.0	41.0
15	39.4960821777043	41.0	39.0	41.0	37.0	41.0
16	39.44663660027038	41.0	39.0	41.0	37.0	41.0
17	39.41533531967511	40.0	39.0	41.0	37.0	41.0
18	39.44161527725987	41.0	39.0	41.0	37.0	41.0
19	39.46215848869595	41.0	39.0	41.0	37.0	41.0
20	39.458571655882686	41.0	39.0	41.0	37.0	41.0
21	39.424582376298	41.0	39.0	41.0	37.0	41.0
22	39.39624967660202	40.0	39.0	41.0	37.0	41.0
23	39.32747066947329	40.0	39.0	41.0	36.0	41.0
24	39.34254071170634	41.0	39.0	41.0	36.0	41.0
25	39.2900682506386	40.0	39.0	41.0	36.0	41.0
26	39.22991197501728	40.0	39.0	41.0	36.0	41.0
27	39.118178428231154	40.0	39.0	41.0	36.0	41.0
28	39.112796357050804	40.0	39.0	41.0	35.0	41.0
29	39.08014439643916	40.0	39.0	41.0	35.0	41.0
30	39.031756770708704	40.0	39.0	41.0	35.0	41.0
31	38.94040853211934	40.0	38.0	41.0	35.0	41.0
32	38.88703845186806	40.0	38.0	41.0	35.0	41.0
33	38.81772743228684	40.0	38.0	41.0	35.0	41.0
34	38.782721498768964	40.0	38.0	41.0	35.0	41.0
35	38.7063740679154	40.0	38.0	41.0	35.0	41.0
36	38.649844708237325	40.0	38.0	41.0	35.0	41.0
37	38.59974565432066	40.0	38.0	41.0	35.0	41.0
38	38.553488507680775	40.0	38.0	41.0	35.0	41.0
39	38.47334532588192	40.0	38.0	41.0	35.0	41.0
40	38.14550662039697	40.0	38.0	41.0	34.0	41.0
41	38.203205678686345	40.0	37.0	41.0	34.0	41.0
42	38.17433975189757	40.0	37.0	41.0	34.0	41.0
43	37.69472931133539	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	4.0
17	2.0
18	10.0
19	16.0
20	30.0
21	75.0
22	119.0
23	227.0
24	393.0
25	682.0
26	1155.0
27	1762.0
28	2691.0
29	3989.0
30	5396.0
31	7437.0
32	10294.0
33	13880.0
34	20142.0
35	30093.0
36	46535.0
37	85907.0
38	191483.0
39	400964.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.75157326285181	17.114889182292	13.013170344799944	27.12036721005625
2	19.9490094001013	20.18902232387412	33.76007695960956	26.101891316415017
3	20.902623501589357	20.799257611847118	29.728321403541162	28.569797483022363
4	15.175473011299797	16.663528845885224	34.53307404811676	33.627924094698216
5	16.47525959899865	33.35936712381679	33.69205710266991	16.47331617451466
6	32.657183564944994	34.57740841915779	16.039082266373047	16.726325749524165
7	29.329312064171877	28.68822491251553	20.757595449471573	21.22486757384102
8	26.72014323038447	31.45311063308267	20.55657247940881	21.270173657124047
9	27.993086267398205	13.648913079125313	18.651530628977188	39.706470024499296
10	18.968430283897877	24.232559890876715	30.547110431452385	26.251899393773027
11	37.31945890203804	20.67572869308347	19.889856417369842	22.11495598750864
12	22.25876939932393	24.575331384240528	27.69914331419465	25.46675590224089
13	33.48398921885268	18.70764701095241	22.1834617005693	25.62490206962561
14	23.53001193991417	20.908575239071578	24.46091226774559	31.100500553268656
15	28.508579611776668	25.080986142168786	21.302968945291386	25.10746530076316
16	24.539135103226208	25.02450536810282	23.698118157779348	26.73824137089163
17	25.349421649019966	24.742708817924203	23.389478056915618	26.518391476140213
18	25.339825990630267	22.598747219992006	25.124470264998074	26.936956524379653
19	26.859219545020036	23.452517888615056	25.13965326877925	24.54860929758566
20	28.139450423848732	22.073901145284342	24.621852107826047	25.16479632304088
21	26.171976061868918	23.503168389229057	23.72362560413172	26.601229944770306
22	26.696700672546335	24.034087665449192	23.31854306324996	25.950668598754508
23	26.447092090383816	23.279310181479406	24.335925780618954	25.93767194751782
24	26.422799284333937	23.046828027582052	24.399330004409144	26.13104268367487
25	26.45523018041052	23.360934009807004	24.651124939116155	25.532710870666314
26	26.27133363861293	24.02218419048475	24.144377004915647	25.562105165986672
27	25.993059545311546	23.086182373382858	25.148034286866462	25.772723794439134
28	25.26014558678666	24.031415456783705	24.05838047149907	26.650058484930568
29	25.90341909098749	24.49322169979193	24.019269053758766	25.584090155461816
30	25.660612494518936	23.411948902511753	25.278365191324063	25.64907341164524
31	26.300363541842536	23.59341616370436	24.377345014934	25.728875279519098
32	24.290376769275433	23.54070077457612	24.75133276407191	27.41758969207654
33	24.50354614236313	23.306396660225023	26.046989574742284	26.143067622669562
34	25.16673974752487	23.118005949308202	25.48667600320179	26.22857829996514
35	24.87291825835156	24.206323660342843	25.68247601996383	25.238282061341767
36	24.995839856963958	23.220643054868948	26.140395414004075	25.643121674163023
37	25.455338283397445	22.790417459725564	25.71065567498169	26.0435885818953
38	24.649060050601914	22.365779209973656	26.769336162635476	26.215824576788954
39	24.264262002771808	21.79635583616446	27.416860907895042	26.52252125316869
40	24.028014463936724	22.619639033194904	27.9328401083945	25.419506394473874
41	22.62498345052588	22.011104241645402	28.852201353352225	26.5117109544765
42	21.62897840248078	22.519188280178646	29.144808202223032	26.70702511511753
43	21.062105773306822	23.116548380945208	28.85608820232021	26.965257643427766
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	32.5
2	37.0
3	78.0
4	119.0
5	119.0
6	163.0
7	207.0
8	219.0
9	231.0
10	348.5
11	466.0
12	466.0
13	997.5
14	1529.0
15	2890.5
16	4252.0
17	4201.0
18	4150.0
19	4150.0
20	4750.0
21	5350.0
22	4557.5
23	3765.0
24	3976.5
25	4188.0
26	4188.0
27	4719.5
28	5251.0
29	6764.0
30	8277.0
31	9586.0
32	10895.0
33	10895.0
34	13229.5
35	15564.0
36	17405.0
37	19246.0
38	22890.5
39	26535.0
40	26535.0
41	30527.0
42	34519.0
43	38837.0
44	43155.0
45	53422.0
46	63689.0
47	63689.0
48	69420.0
49	75151.0
50	83130.5
51	91110.0
52	91382.0
53	91654.0
54	91654.0
55	82637.5
56	73621.0
57	68202.0
58	62783.0
59	56649.5
60	50516.0
61	50516.0
62	45962.0
63	41408.0
64	34123.0
65	26838.0
66	22985.0
67	19132.0
68	19132.0
69	16350.0
70	13568.0
71	11644.5
72	9721.0
73	7934.0
74	6147.0
75	6147.0
76	4994.0
77	3841.0
78	3170.5
79	2500.0
80	2009.0
81	1518.0
82	1518.0
83	1266.0
84	1014.0
85	810.0
86	606.0
87	494.0
88	382.0
89	382.0
90	308.5
91	235.0
92	154.0
93	73.0
94	44.0
95	15.0
96	15.0
97	9.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	823289.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.13291465526841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.6417434114156	40.36544983138647
2	6.866578274565513	6.472836955776332
3	2.2292404689818044	3.1521180231176977
4	1.1840286581051507	2.2322688676744815
5	0.7008715644547959	1.6517059815876176
6	0.4993252387772976	1.4120792318707132
7	0.34404922709400626	1.1351230000483001
8	0.2869718834204136	1.0820657031772791
9	0.2226052020004915	0.9442828788937154
>10	1.6508259297998398	15.873203959694113
>50	0.21268802711092377	6.982503758792929
>100	0.14680646808238146	12.985886986087078
>500	0.011153141568058146	3.528902532781804
>1k	0.0031125046236441336	2.181572289111456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2294	0.27863848539212843	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2234	0.27135064357716426	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1777	0.2158415817531875	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1606	0.19507123258053977	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1484	0.1802526208901127	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1468	0.17830919640612228	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1324	0.16081837605020835	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1210	0.14697147660177654	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1155	0.1402909549380594	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1140	0.13846899448431838	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1104	0.1340962893953399	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1049	0.1274157677316228	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	994	0.12073524606790569	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	993	0.12061378203765628	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	956	0.11611961291842841	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	891	0.10822445095221728	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	887	0.10773859483121966	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	865	0.1050663861657328	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	848	0.10300149765149298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.6439209074820626E-4	0.0	0.0	0.0	0.0
9	3.6439209074820626E-4	0.0	0.0	0.0	0.0
10	3.6439209074820626E-4	0.0	0.0	0.0	0.0
11	3.6439209074820626E-4	0.0	0.0	0.0	0.0
12	3.6439209074820626E-4	0.0	0.0	0.0	0.0
13	3.6439209074820626E-4	0.0	0.0	0.0	0.0
14	3.6439209074820626E-4	0.0	0.0	0.0	0.0
15	4.8585612099760837E-4	0.0	0.0	0.0	0.0
16	4.8585612099760837E-4	0.0	0.0	6.073201512470104E-4	0.0
17	7.287841814964125E-4	0.0	0.0	0.001457568362992825	0.0
18	7.287841814964125E-4	0.0	0.0	0.0024292806049880417	0.0
19	8.502482117458146E-4	0.0	0.0	0.002793672695736248	0.0
20	8.502482117458146E-4	0.0	0.0	0.004008312998230269	0.0
21	8.502482117458146E-4	0.0	0.0	0.005101489270474888	0.0
22	8.502482117458146E-4	0.0	0.0	0.007166377784714724	0.0
23	8.502482117458146E-4	0.0	0.0	0.008988338238455755	0.0
24	8.502482117458146E-4	0.0	0.0	0.011782010934192002	1.2146403024940209E-4
25	8.502482117458146E-4	0.0	0.0	0.013239579297184828	1.2146403024940209E-4
26	8.502482117458146E-4	0.0	0.0	0.017126428265165694	1.2146403024940209E-4
27	8.502482117458146E-4	0.0	0.0	0.03850409758906046	1.2146403024940209E-4
28	8.502482117458146E-4	0.0	0.0	0.12522941518713357	1.2146403024940209E-4
29	8.502482117458146E-4	0.0	0.0	0.2601759527942193	1.2146403024940209E-4
30	8.502482117458146E-4	0.0	0.0	0.44419395862206346	1.2146403024940209E-4
31	8.502482117458146E-4	0.0	0.0	1.009487555402781	1.2146403024940209E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1255	0.0	27.270918	1
ACCTCTA	60	3.724634E-5	21.583332	31
CGTTCGT	45	0.003824899	20.555557	14
GTATGGA	45	0.003824899	20.555557	1
GTCTATA	55	5.141392E-4	20.181818	2
GGCTTAT	55	5.141392E-4	20.181818	17
ATAACGC	140	3.6379788E-12	19.821428	35
TATACAC	245	0.0	19.632654	37
CTTATAC	825	0.0	19.28485	37
GCGGTAA	630	0.0	18.5	23
TATTAGA	130	6.9485395E-10	18.5	2
GTATAGA	60	9.233783E-4	18.5	1
TGTACAC	60	9.233783E-4	18.5	5
GTACTGG	395	0.0	17.79747	1
GTATCAA	1910	0.0	17.72513	2
ATACGGC	115	6.398295E-8	17.695652	29
TACGGCT	105	4.7931826E-7	17.619047	30
GTATTAG	195	0.0	17.076921	1
TCTTATA	1525	0.0	16.983606	37
CGCGGTA	700	0.0	16.65	22
>>END_MODULE