FastQCFastQC Report
Fri 10 Feb 2017
ERR1631878.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631878.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797459
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19760.247787033565362No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA18580.23299003459738996No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA18140.22747250955848514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17950.22508994192804893No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17140.21493267992461054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.20765957873696328No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16310.20452462132849464No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15570.19524514739942744No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC14940.18734505473008645No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14180.17781478420834174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13300.1667797341305321No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13020.1632685818330472No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC13000.16301778524036972No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA12170.15260972664425382No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT11840.1484715828650752No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11780.14771919308704273No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10630.13329838900808694No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10060.1261506861167784No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9220.11561722922432377No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8890.11147908544514515No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA8890.11147908544514515No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC8820.11060129737077391No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA8810.11047589907443517No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8700.10909651781470897No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8570.10746633996230527No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC8430.10571076381356284No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8410.10545996722088533No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT8410.10545996722088533No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG8300.10408058596115913No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT8170.10245040810875543No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8070.10119642514536797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.028.7777791
CGTTATT502.701097E-422.19999910
CATAGGA450.003824796820.5555552
GGACCGT1800.020.5555556
TGTTACG555.1411963E-420.18181816
GTATCAA17550.019.8176632
TTTAGGA851.2443288E-619.5882362
GACCGTT1153.0468073E-919.3043467
CTTATAC6300.018.7936537
TATTAGA801.6156586E-518.52
CAGTCGG2900.018.510
TTGTTAC701.21860314E-418.515
TCTGACG500.0070330418.4999988
TAAGTCT1002.8721843E-718.49999835
TATACAC2050.018.0487837
GCGGTAA7200.017.9861123
CTTAGGA1653.6379788E-1217.9393942
GCAGTCG3000.017.8833339
CTCTATG4600.017.695651
ATACGGC953.6035835E-617.52631629
Produced by FastQC (version 0.11.5)