##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631878.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	797459
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61318262130091	34.0	31.0	34.0	31.0	34.0
2	32.782070300792896	34.0	31.0	34.0	31.0	34.0
3	32.86780386201673	34.0	31.0	34.0	31.0	34.0
4	36.266796161307354	37.0	37.0	37.0	35.0	37.0
5	36.200094299518845	37.0	35.0	37.0	35.0	37.0
6	36.26110182467061	37.0	37.0	37.0	35.0	37.0
7	36.257472797974565	37.0	37.0	37.0	35.0	37.0
8	36.24262814765399	37.0	37.0	37.0	35.0	37.0
9	38.06704545312047	39.0	38.0	39.0	37.0	39.0
10	38.00286033513949	39.0	38.0	39.0	35.0	39.0
11	38.0699935670674	39.0	38.0	39.0	37.0	39.0
12	38.0080417927442	39.0	38.0	39.0	35.0	39.0
13	38.058998644444415	39.0	38.0	39.0	35.0	39.0
14	39.45261888071989	40.0	39.0	41.0	37.0	41.0
15	39.46065440354927	41.0	39.0	41.0	37.0	41.0
16	39.41167382899936	40.0	39.0	41.0	37.0	41.0
17	39.3836661195121	40.0	39.0	41.0	37.0	41.0
18	39.417358133772396	40.0	39.0	41.0	37.0	41.0
19	39.42954684817652	41.0	39.0	41.0	37.0	41.0
20	39.423843733659034	40.0	39.0	41.0	37.0	41.0
21	39.38857546281376	40.0	39.0	41.0	37.0	41.0
22	39.36133895284899	40.0	39.0	41.0	36.0	41.0
23	39.29800027336829	40.0	39.0	41.0	36.0	41.0
24	39.308240298247306	40.0	39.0	41.0	36.0	41.0
25	39.25500872145151	40.0	39.0	41.0	36.0	41.0
26	39.19561005644177	40.0	39.0	41.0	36.0	41.0
27	39.08170325997951	40.0	39.0	41.0	35.0	41.0
28	39.07246140553934	40.0	39.0	41.0	35.0	41.0
29	39.0455459152132	40.0	39.0	41.0	35.0	41.0
30	39.001875958513224	40.0	39.0	41.0	35.0	41.0
31	38.90757393170056	40.0	38.0	41.0	35.0	41.0
32	38.861918919969554	40.0	38.0	41.0	35.0	41.0
33	38.79758207005	40.0	38.0	41.0	35.0	41.0
34	38.762901917214556	40.0	38.0	41.0	35.0	41.0
35	38.6923553436603	40.0	38.0	41.0	35.0	41.0
36	38.640825421745824	40.0	38.0	41.0	35.0	41.0
37	38.58600128658652	40.0	38.0	41.0	35.0	41.0
38	38.55000946757137	40.0	38.0	41.0	35.0	41.0
39	38.454721810149486	40.0	38.0	41.0	34.0	41.0
40	38.12784732506624	40.0	38.0	41.0	34.0	41.0
41	38.19183682170494	40.0	37.0	41.0	34.0	41.0
42	38.173628989076555	40.0	37.0	41.0	34.0	41.0
43	37.69746156228722	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	12.0
19	11.0
20	34.0
21	40.0
22	143.0
23	259.0
24	416.0
25	667.0
26	1088.0
27	1770.0
28	2679.0
29	3904.0
30	5403.0
31	7534.0
32	9908.0
33	13665.0
34	19619.0
35	29642.0
36	45803.0
37	83649.0
38	190417.0
39	380794.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.91129700711886	16.763369652859897	12.915522929705478	27.409810410315767
2	20.500364282050864	19.87951731687773	33.06502277860053	26.555095622470876
3	21.278586109129122	20.20956563284131	29.59700749505617	28.91484076297339
4	15.182974924102682	16.579410352130957	34.793763691926486	33.443851031839884
5	16.614647273402145	33.011101511174864	33.753208628907565	16.62104258651542
6	32.675410271876046	34.39085896579009	16.183653328885875	16.750077433447988
7	29.605910774096223	28.262393427122902	20.561307854071494	21.57038794470938
8	26.890912260066035	30.844218950441338	20.646202500692826	21.6186662887998
9	28.3141829235108	13.187135639575201	18.365583685180052	40.13309775173394
10	19.17891703523316	23.66140453615797	30.112645289601097	27.047033139007777
11	38.072803742888354	20.216337140843603	19.586085303445067	22.12477381282298
12	22.2850328355439	24.315858244749887	27.473011151670494	25.92609776803572
13	34.42308632794915	18.240686982026663	21.480728162827177	25.855498527197007
14	23.635948682001207	20.806085328524727	24.0984175988985	31.459548390575563
15	29.21817924181682	24.5385656190475	20.778121508441185	25.465133630694492
16	24.63148575663451	24.62559203670659	23.561085899086976	27.181836307571928
17	26.024159235772622	24.342066488684686	22.77797353845151	26.855800737091183
18	25.943653529523147	21.99874852500254	24.431851668863228	27.625746276611086
19	27.173309223420887	23.086829542333838	24.54270376282668	25.197157471418592
20	29.003622756781226	21.41263688791524	23.94329990632747	25.640440448976058
21	26.650774522577336	22.96669797444132	23.13059354775606	27.251933955225283
22	27.437021840621274	23.383521911471313	22.66787383426609	26.511582413641328
23	27.09944962687737	22.6666198513027	23.630180360369625	26.603750161450307
24	27.062833324346457	22.31262046073842	23.92988228861923	26.6946639262959
25	27.066595273236615	22.71803315280159	24.089514319858452	26.125857254103344
26	26.948093883196506	23.445844864751667	23.566101830940525	26.039959421111302
27	26.363487025665272	22.46334921293759	25.021223661655334	26.151940099741804
28	25.643951601273546	23.523968003370708	23.273422207285893	27.558658188069856
29	26.43646883413442	23.949193626255393	23.44973221193817	26.16460532767202
30	26.19959145235053	22.521157827549754	24.807670362990446	26.47158035710927
31	27.07725412842541	22.969080542071755	23.833325600438393	26.12033972906444
32	24.97219292778688	22.889076429007634	24.02443260405864	28.11429803914684
33	25.149882313698885	22.65094506426036	25.492846654185357	26.7063259678554
34	25.591660511700287	22.552256605041766	24.82648010744126	27.029602775816684
35	25.135085314730915	24.168515246551863	25.118532739614203	25.577866699103026
36	25.670912234986375	22.954910534585476	25.4567319448398	25.917445285588347
37	26.193697732422606	22.718158551097925	24.99363603646081	26.09450768001866
38	25.464381240916463	22.217066958928296	25.76671653338918	26.551835266766066
39	24.987115325051192	21.367995094418646	26.72062137363802	26.924268206892144
40	24.797262304394334	22.276380353096524	27.12841663333162	25.797940709177524
41	23.220127931341924	21.72964378105959	27.99040452236416	27.059823765234327
42	21.985957898775986	22.228478203895122	28.59745767494003	27.188106222388864
43	21.489380645274554	22.957418500512254	28.226780310962695	27.326420543250503
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	11.5
2	14.0
3	34.0
4	54.0
5	54.0
6	93.0
7	132.0
8	140.0
9	148.0
10	231.0
11	314.0
12	314.0
13	662.0
14	1010.0
15	1971.0
16	2932.0
17	2960.5
18	2989.0
19	2989.0
20	3400.0
21	3811.0
22	3128.5
23	2446.0
24	2623.5
25	2801.0
26	2801.0
27	3227.5
28	3654.0
29	4679.5
30	5705.0
31	6756.5
32	7808.0
33	7808.0
34	9816.0
35	11824.0
36	13268.0
37	14712.0
38	18201.5
39	21691.0
40	21691.0
41	25802.5
42	29914.0
43	34407.0
44	38900.0
45	51379.0
46	63858.0
47	63858.0
48	69550.0
49	75242.0
50	84535.0
51	93828.0
52	93236.5
53	92645.0
54	92645.0
55	83387.0
56	74129.0
57	68651.5
58	63174.0
59	57838.5
60	52503.0
61	52503.0
62	47675.5
63	42848.0
64	35127.5
65	27407.0
66	23712.0
67	20017.0
68	20017.0
69	17269.5
70	14522.0
71	12378.0
72	10234.0
73	8240.5
74	6247.0
75	6247.0
76	5127.5
77	4008.0
78	3268.5
79	2529.0
80	2024.0
81	1519.0
82	1519.0
83	1227.0
84	935.0
85	706.5
86	478.0
87	380.5
88	283.0
89	283.0
90	210.0
91	137.0
92	88.5
93	40.0
94	23.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	797459.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.671977175944114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.14539118440078	39.34430339986729
2	6.596711464742014	6.025697109079721
3	2.1110791561470315	2.892514771084757
4	1.1462111292047332	2.093989141274068
5	0.6628034913448795	1.51357729644197
6	0.47611962187758505	1.3047194702047322
7	0.34029129115824175	1.0879243258066238
8	0.282602737499968	1.0325620621566292
9	0.20489164608214866	0.842202592506496
>10	1.6344042846369655	15.127204455791205
>50	0.22154262596518884	6.960954453251144
>100	0.15836292189740211	13.881583434018657
>500	0.014622360384250915	4.554488982286092
>1k	0.004966084658802197	3.338278506230611
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1976	0.247787033565362	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1858	0.23299003459738996	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1814	0.22747250955848514	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1795	0.22508994192804893	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1714	0.21493267992461054	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1656	0.20765957873696328	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1631	0.20452462132849464	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1557	0.19524514739942744	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1494	0.18734505473008645	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1418	0.17781478420834174	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1330	0.1667797341305321	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1302	0.1632685818330472	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1300	0.16301778524036972	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1217	0.15260972664425382	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1184	0.1484715828650752	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1178	0.14771919308704273	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1063	0.13329838900808694	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1006	0.1261506861167784	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	922	0.11561722922432377	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	889	0.11147908544514515	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	889	0.11147908544514515	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	882	0.11060129737077391	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	881	0.11047589907443517	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	870	0.10909651781470897	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	857	0.10746633996230527	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	843	0.10571076381356284	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	841	0.10545996722088533	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	841	0.10545996722088533	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	830	0.10408058596115913	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	817	0.10245040810875543	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	807	0.10119642514536797	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.2539829633874595E-4	0.0	0.0	0.0	0.0
8	2.507965926774919E-4	0.0	0.0	0.0	0.0
9	2.507965926774919E-4	0.0	0.0	0.0	0.0
10	2.507965926774919E-4	0.0	0.0	0.0	0.0
11	2.507965926774919E-4	1.2539829633874595E-4	0.0	0.0	0.0
12	2.507965926774919E-4	1.2539829633874595E-4	0.0	0.0	0.0
13	2.507965926774919E-4	1.2539829633874595E-4	0.0	0.0	0.0
14	3.7619488901623785E-4	1.2539829633874595E-4	0.0	1.2539829633874595E-4	0.0
15	3.7619488901623785E-4	1.2539829633874595E-4	0.0	1.2539829633874595E-4	0.0
16	5.015931853549838E-4	1.2539829633874595E-4	0.0	3.7619488901623785E-4	0.0
17	6.269914816937297E-4	1.2539829633874595E-4	0.0	7.523897780324757E-4	0.0
18	6.269914816937297E-4	1.2539829633874595E-4	0.0	0.0012539829633874595	0.0
19	7.523897780324757E-4	1.2539829633874595E-4	0.0	0.002006372741419935	0.0
20	7.523897780324757E-4	1.2539829633874595E-4	0.0	0.002507965926774919	0.0
21	7.523897780324757E-4	1.2539829633874595E-4	0.0	0.00401274548283987	0.0
22	7.523897780324757E-4	1.2539829633874595E-4	0.0	0.006520711409614789	0.0
23	8.777880743712216E-4	1.2539829633874595E-4	0.0	0.007649296076663503	0.0
24	0.0010031863707099676	1.2539829633874595E-4	0.0	0.010282660299777167	0.0
25	0.0010031863707099676	1.2539829633874595E-4	0.0	0.012038236448519611	0.0
26	0.0010031863707099676	1.2539829633874595E-4	0.0	0.01479699896797202	0.0
27	0.0011285846670487135	1.2539829633874595E-4	0.0	0.02934320134326655	0.0
28	0.0011285846670487135	1.2539829633874595E-4	0.0	0.1020742132197392	0.0
29	0.0011285846670487135	1.2539829633874595E-4	0.0	0.20979134977472197	0.0
30	0.0011285846670487135	1.2539829633874595E-4	0.0	0.3630280679006695	0.0
31	0.0011285846670487135	1.2539829633874595E-4	0.0	0.7992887408631666	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1215	0.0	28.777779	1
CGTTATT	50	2.701097E-4	22.199999	10
CATAGGA	45	0.0038247968	20.555555	2
GGACCGT	180	0.0	20.555555	6
TGTTACG	55	5.1411963E-4	20.181818	16
GTATCAA	1755	0.0	19.817663	2
TTTAGGA	85	1.2443288E-6	19.588236	2
GACCGTT	115	3.0468073E-9	19.304346	7
CTTATAC	630	0.0	18.79365	37
TATTAGA	80	1.6156586E-5	18.5	2
CAGTCGG	290	0.0	18.5	10
TTGTTAC	70	1.21860314E-4	18.5	15
TCTGACG	50	0.00703304	18.499998	8
TAAGTCT	100	2.8721843E-7	18.499998	35
TATACAC	205	0.0	18.04878	37
GCGGTAA	720	0.0	17.98611	23
CTTAGGA	165	3.6379788E-12	17.939394	2
GCAGTCG	300	0.0	17.883333	9
CTCTATG	460	0.0	17.69565	1
ATACGGC	95	3.6035835E-6	17.526316	29
>>END_MODULE