FastQCFastQC Report
Fri 10 Feb 2017
ERR1631877.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631877.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292120
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16900.578529371491168No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16380.5607284677529782No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13520.4628234971929345No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12450.42619471450089No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10220.34985622346980694No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA10090.34540599753525947No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9950.34061344652882375No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9780.3347939203067233No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC9770.33445159523483503No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9080.3108311652745447No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8900.30466931398055597No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8720.2985074626865672No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC8430.2885800356018075No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8060.27591400794194165No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6950.23791592496234426No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC6880.23551964945912637No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6830.23380802409968507No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6770.2317540736683555No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6670.22833082294947282No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6630.22696152266191977No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT6610.22627687251814324No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6090.20847596877995345No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6090.20847596877995345No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA5950.20368341777351773No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5930.20299876762974123No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5920.20265644255785292No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5910.20231411748596467No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA5870.20094481719841162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5810.19889086676708204No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5690.19478296590442284No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG5650.1934136656168698No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5630.19272901547309323No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5590.19135971518554018No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC5550.18999041489798713No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA5450.18656716417910446No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC5360.1834862385321101No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5330.1824592633164453No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG5330.1824592633164453No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC5320.18211693824455702No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5280.180747637957004No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT5150.17629741202245652No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5090.17424346159112694No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA5040.1725318362316856No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4970.17013556072846775No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4950.16945091058469122No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC4890.16739696015336164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.16636998493769684No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4800.16431603450636723No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG4800.16431603450636723No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4780.1636313843625907No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4740.16226208407503767No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4620.15815418321237848No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC4490.15370395727783104No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA4460.15267698206216623No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA4410.1509653567027249No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4340.14856908119950707No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4270.14617280569628918No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC4250.14548815555251265No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC4140.14172257976174174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4080.13966862933041216No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3940.13487607832397647No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3920.1341914281801999No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG3890.13316445296453513No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3880.13282212789264686No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3880.13282212789264686No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3800.13008352731754075No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT3760.12871422702998767No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3760.12871422702998767No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG3730.12768725181432286No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3710.12700260167054633No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3680.12597562645488156No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG3660.12529097631110503No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3650.12494865123921675No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3640.1246063261673285No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3560.12186772559222238No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA3540.12118307544844585No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA3520.12049842530466931No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA3510.12015610023278106No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT3490.1194714500890045No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC3460.11844447487333973No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3430.11741749965767494No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG3370.11536354922634534No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA3360.11502122415445706No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT3320.11365192386690401No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG3310.11330959879501575No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA3270.11194029850746269No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3220.11022867314802137No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT3180.1088593728604683No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG3170.10851704778858003No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC3100.10612077228536218No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC3070.10509379706969738No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3060.10475147199780913No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2990.10235519649459127No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2950.10098589620703821No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2940.10064357113514993No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2940.10064357113514993No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2940.10064357113514993No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2940.10064357113514993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGC200.001839689737.025
CTAAGTC303.5929662E-430.83333234
GGTATCA6750.030.148151
TTGGAGC250.005489716329.633
ATACACA453.9948136E-628.77777937
AACCCTG604.299727E-827.74999836
TAAGTCT358.853502E-426.4285735
TACGGCT1050.026.4285730
TCGCTTA358.853502E-426.4285723
AAACCAT358.853502E-426.428576
TCTCAGA358.853502E-426.4285723
ATACGGC1050.026.4285729
CTCTCAG358.853502E-426.4285722
CTACCGT509.06081E-625.9000028
ACCGTTT509.06081E-625.90000210
CCGTTTC509.06081E-625.90000211
AACGACC659.341784E-825.61538537
GGAGGGC659.341784E-825.61538533
TGATGGG451.3199137E-424.66666833
GTATTAG601.3317094E-624.6666661
Produced by FastQC (version 0.11.5)