FastQCFastQC Report
Fri 10 Feb 2017
ERR1631876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121408
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3530.2907551396942541No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2600.21415392725355828No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2470.20344623089088035No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2330.1919148655772272No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2200.18120716921454927No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1940.15979177648919346No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1870.1540260938323669No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1860.15320242488139166No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1810.14908408012651556No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1720.14167105956773854No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1640.13508170795993674No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1620.1334343700579863No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1610.13261070110701106No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1590.13096336320506063No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1410.11613732208750659No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1370.11284264628360569No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1350.11119530838165524No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1310.10790063257775435No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1260.10378228782287824No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1260.10378228782287824No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1260.10378228782287824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT303.580713E-430.8333326
TGCGGGT502.6870293E-729.59999821
TAGGACC250.005477240329.5999984
TTAGGAC405.8971396E-527.7500023
CGGGTAA556.184546E-726.9090923
TAGAACA556.184546E-726.909094
TTCTGCG556.184546E-726.9090918
ACCGTTA451.3137129E-424.6666688
GTAACGT601.3210192E-624.66666626
CTGTAGA400.001921308523.1250021
TAAGTCC400.001921308523.12500237
GTAGAAC400.001921308523.1250023
TTACAAC400.001921308523.12500223
AGTCGGT400.001921308523.12500211
GGTATCA1200.023.1249981
ACGTCAA652.6501766E-622.7692329
CTTAGGA502.6828618E-422.1999992
AATGAGC603.6910747E-521.58333234
GGTAACG705.040187E-621.14285725
TAACGTC705.040187E-621.14285727
Produced by FastQC (version 0.11.5)