##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631876.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	121408
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.586633500263574	34.0	31.0	34.0	31.0	34.0
2	32.76899380600949	34.0	31.0	34.0	31.0	34.0
3	32.85755469161835	34.0	31.0	34.0	31.0	34.0
4	36.27059996046389	37.0	37.0	37.0	35.0	37.0
5	36.187903597785976	37.0	35.0	37.0	35.0	37.0
6	36.24911043753295	37.0	37.0	37.0	35.0	37.0
7	36.241466789667896	37.0	37.0	37.0	35.0	37.0
8	36.22874110437533	37.0	37.0	37.0	35.0	37.0
9	38.06731846336321	39.0	38.0	39.0	35.0	39.0
10	37.99528861360042	39.0	38.0	39.0	35.0	39.0
11	38.07324064312072	39.0	38.0	39.0	37.0	39.0
12	38.011177187664735	39.0	38.0	39.0	35.0	39.0
13	38.04883533210332	39.0	38.0	39.0	35.0	39.0
14	39.4528696626252	40.0	39.0	41.0	37.0	41.0
15	39.465010542962574	41.0	39.0	41.0	37.0	41.0
16	39.40951172904586	40.0	39.0	41.0	37.0	41.0
17	39.36206839746969	40.0	39.0	41.0	36.0	41.0
18	39.381045730100155	40.0	39.0	41.0	36.0	41.0
19	39.40529454401687	41.0	39.0	41.0	37.0	41.0
20	39.40452029520295	40.0	39.0	41.0	37.0	41.0
21	39.353856418028464	40.0	39.0	41.0	36.0	41.0
22	39.34174024775962	40.0	39.0	41.0	36.0	41.0
23	39.26577326041117	40.0	39.0	41.0	36.0	41.0
24	39.281694781233526	40.0	39.0	41.0	36.0	41.0
25	39.229886004217185	40.0	39.0	41.0	36.0	41.0
26	39.17217975751186	40.0	39.0	41.0	36.0	41.0
27	39.04988962836057	40.0	39.0	41.0	35.0	41.0
28	39.04364621771218	40.0	39.0	41.0	35.0	41.0
29	39.03636498418555	40.0	39.0	41.0	35.0	41.0
30	38.99320473115446	40.0	39.0	41.0	35.0	41.0
31	38.90657946758039	40.0	38.0	41.0	35.0	41.0
32	38.86084936742225	40.0	38.0	41.0	35.0	41.0
33	38.81261531365314	40.0	38.0	41.0	35.0	41.0
34	38.7638458750659	40.0	38.0	41.0	35.0	41.0
35	38.7014364786505	40.0	38.0	41.0	35.0	41.0
36	38.658325645756456	40.0	38.0	41.0	35.0	41.0
37	38.60925968634686	40.0	38.0	41.0	35.0	41.0
38	38.57216163679494	40.0	38.0	41.0	35.0	41.0
39	38.492916447021614	40.0	38.0	41.0	34.0	41.0
40	38.20207070374275	40.0	37.0	41.0	34.0	41.0
41	38.266152148128626	40.0	37.0	41.0	34.0	41.0
42	38.2657979704797	40.0	37.0	41.0	34.0	41.0
43	37.79610898787559	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	4.0
19	3.0
20	5.0
21	12.0
22	24.0
23	30.0
24	65.0
25	99.0
26	147.0
27	262.0
28	366.0
29	595.0
30	800.0
31	1162.0
32	1546.0
33	2052.0
34	3159.0
35	4548.0
36	7030.0
37	12554.0
38	28982.0
39	57961.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.1017725355825	16.292171850289932	12.53294675803901	28.073108856088563
2	21.341262519768055	19.61402872957301	31.743377701634156	27.301331049024775
3	21.19382577754349	19.602497364259357	29.25095545598313	29.95272140221402
4	15.829269899841854	15.671125461254611	33.53815234580917	34.96145229309436
5	16.934633632050605	32.97146810753822	33.44260674749604	16.65129151291513
6	34.95733394833948	33.34047179757512	15.334244860305745	16.36794939377965
7	30.662724037954664	27.51548497627833	19.704632314180284	22.117158671586715
8	27.704928835002633	30.50046125461255	20.31991302055878	21.474696889826042
9	28.136531365313655	12.735569319978914	17.155376910911965	41.972522403795466
10	19.16924749604639	23.764496573537166	29.647140221402214	27.419115709014235
11	39.55834870848708	19.32244992092778	18.642099367422247	22.477102003162887
12	23.182986294148655	23.3510147601476	26.410121244069586	27.055877701634156
13	35.36505007907222	17.300342646283607	20.75398655772272	26.580620716921455
14	24.47944122298366	19.491302055877703	22.577589615181868	33.45166710595677
15	29.44781233526621	24.11867422245651	19.52095413811281	26.912559304164468
16	26.101245387453876	23.866631523458093	21.946659198734846	28.08546389035319
17	27.794708750658938	23.364193463363208	21.13863995782815	27.70245782814971
18	26.754414865577225	21.276192672641013	23.00260279388508	28.96678966789668
19	28.610964681075384	22.056207169214552	23.001779124934107	26.33104902477596
20	30.213824459673166	20.525830258302584	22.477102003162887	26.783243278861363
21	27.70410516605166	22.131161043753295	21.39315366367949	28.771580126515552
22	28.87206773853453	22.382380073800736	20.753162888771744	27.992389298892988
23	28.260905376910912	22.0578545071165	21.88076568265683	27.800474433315763
24	28.19418819188192	21.39315366367949	22.546290195044808	27.866367949393776
25	28.522832103321033	21.791809435951503	22.443331576172902	27.24202688455456
26	28.339977596204534	22.40791381128097	21.92359646810754	27.328512124406956
27	27.394405640484976	21.529059040590408	23.6137651555087	27.462770163415918
28	26.90267527675277	22.513343437005798	21.804988139167104	28.778993147074328
29	27.499835266209804	23.246408803373747	22.026555086979442	27.227200843437004
30	27.200019768054823	22.1476344227728	23.244761465471797	27.40758434370058
31	28.343272272008434	22.308249868212968	22.264595413811282	27.083882445967316
32	25.68117422245651	21.94418819188192	22.91858856088561	29.45604902477596
33	26.410944913020558	21.765452029520297	23.75955455983131	28.064048497627837
34	26.52625856615709	21.801693463363208	23.44573668950975	28.226311280969952
35	26.148194517659462	23.21099103848181	23.977826831839746	26.66298761201898
36	26.95044807590933	22.040557459146022	24.492619926199264	26.516374538745385
37	27.33674881391671	21.58506852925672	24.039602003162887	27.03858065366368
38	26.040293885081706	21.549650764364785	25.12766868740116	27.282386663152348
39	25.537032156035842	20.915425672113862	25.804724565102795	27.742817606747494
40	25.165557459146022	21.461518186610437	26.815366367949395	26.55755798629415
41	23.857571164997367	20.79928835002636	27.577260147601475	27.765880337374803
42	22.89964417501318	21.07439377965208	28.40587111228255	27.62009093305219
43	21.93348049551924	21.617191618344755	28.209837901950447	28.239489984185557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	2.0
4	3.0
5	3.0
6	8.0
7	13.0
8	8.0
9	3.0
10	9.0
11	15.0
12	15.0
13	33.0
14	51.0
15	90.0
16	129.0
17	125.5
18	122.0
19	122.0
20	147.0
21	172.0
22	178.5
23	185.0
24	225.5
25	266.0
26	266.0
27	305.0
28	344.0
29	494.0
30	644.0
31	791.5
32	939.0
33	939.0
34	1156.0
35	1373.0
36	1585.0
37	1797.0
38	2310.0
39	2823.0
40	2823.0
41	3330.5
42	3838.0
43	4408.0
44	4978.0
45	6613.5
46	8249.0
47	8249.0
48	9194.0
49	10139.0
50	11650.0
51	13161.0
52	13730.5
53	14300.0
54	14300.0
55	12984.5
56	11669.0
57	11258.0
58	10847.0
59	10126.5
60	9406.0
61	9406.0
62	8700.5
63	7995.0
64	6773.0
65	5551.0
66	4798.5
67	4046.0
68	4046.0
69	3447.0
70	2848.0
71	2495.0
72	2142.0
73	1738.5
74	1335.0
75	1335.0
76	1118.5
77	902.0
78	707.5
79	513.0
80	401.0
81	289.0
82	289.0
83	213.0
84	137.0
85	118.0
86	99.0
87	72.0
88	45.0
89	45.0
90	36.0
91	27.0
92	17.5
93	8.0
94	5.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	121408.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.48435028993147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.18496987912103	55.10180548234054
2	5.500850239253372	6.87434106483922
3	1.8744809585953255	3.5137717448603056
4	1.0018322985460248	2.503953610964681
5	0.601099379127615	1.8779652082235108
6	0.46927933984524334	1.7593568792830785
7	0.35195950488393246	1.5394372693726937
8	0.27286748131450944	1.363995782814971
9	0.21354846363744215	1.2009093305218768
>10	1.3946560156074925	16.08213626779125
>50	0.09095582710483648	3.9593766473379017
>100	0.04350061296318267	4.222950711649974
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	353	0.2907551396942541	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	260	0.21415392725355828	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	247	0.20344623089088035	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	233	0.1919148655772272	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	220	0.18120716921454927	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	194	0.15979177648919346	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	187	0.1540260938323669	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	186	0.15320242488139166	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	181	0.14908408012651556	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	172	0.14167105956773854	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	164	0.13508170795993674	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	162	0.1334343700579863	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	161	0.13261070110701106	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	159	0.13096336320506063	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	141	0.11613732208750659	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	137	0.11284264628360569	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	135	0.11119530838165524	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	131	0.10790063257775435	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	126	0.10378228782287824	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	126	0.10378228782287824	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	126	0.10378228782287824	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	8.236689509752241E-4	0.0
20	0.0	0.0	0.0	8.236689509752241E-4	0.0
21	0.0	0.0	0.0	0.00411834475487612	0.0
22	0.0	0.0	0.0	0.00823668950975224	0.0
23	0.0	0.0	0.0	0.00823668950975224	0.0
24	0.0	0.0	0.0	0.009060358460727464	0.0
25	0.0	0.0	0.0	0.009060358460727464	0.0
26	0.0	0.0	0.0	0.01235503426462836	0.0
27	0.0	0.0	0.0	0.029652082235108065	0.0
28	0.0	0.0	0.0	0.09554559831312599	0.0
29	0.0	0.0	0.0	0.21827227200843438	0.0
30	0.0	0.0	0.0	0.3599433315761729	0.0
31	0.0	0.0	0.0	0.7767198207696363	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	30	3.580713E-4	30.833332	6
TGCGGGT	50	2.6870293E-7	29.599998	21
TAGGACC	25	0.0054772403	29.599998	4
TTAGGAC	40	5.8971396E-5	27.750002	3
CGGGTAA	55	6.184546E-7	26.90909	23
TAGAACA	55	6.184546E-7	26.90909	4
TTCTGCG	55	6.184546E-7	26.90909	18
ACCGTTA	45	1.3137129E-4	24.666668	8
GTAACGT	60	1.3210192E-6	24.666666	26
CTGTAGA	40	0.0019213085	23.125002	1
TAAGTCC	40	0.0019213085	23.125002	37
GTAGAAC	40	0.0019213085	23.125002	3
TTACAAC	40	0.0019213085	23.125002	23
AGTCGGT	40	0.0019213085	23.125002	11
GGTATCA	120	0.0	23.124998	1
ACGTCAA	65	2.6501766E-6	22.76923	29
CTTAGGA	50	2.6828618E-4	22.199999	2
AATGAGC	60	3.6910747E-5	21.583332	34
GGTAACG	70	5.040187E-6	21.142857	25
TAACGTC	70	5.040187E-6	21.142857	27
>>END_MODULE