FastQCFastQC Report
Fri 10 Feb 2017
ERR1631875.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631875.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185820
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5130.27607361963190186No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4990.2685394467764503No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4250.22871596168334948No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4170.22441072005166288No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3920.21095683995264233No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3730.2007318910773867No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3630.1953503390377785No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3410.1835109245506404No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3380.18189645893875792No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3000.16144656118824668No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2810.15122161231299105No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2750.1479926810892261No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2280.1226993865030675No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2260.12162307609514583No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2250.12108492089118503No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2190.11785598966742009No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2060.1108599720159294No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1990.10709288558820364No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1990.10709288558820364No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1990.10709288558820364No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1950.10494026477236035No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1920.10332579916047789No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1870.10063502314067378No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1860.10009686793671295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAG303.587976E-430.8333342
GGTATCA2650.030.0188671
TTAGAAC250.005484638729.5999983
TAGATAA250.005484638729.5999984
GGTAGCG250.005484638729.5999988
TTAGGAC358.8413234E-426.428573
TCCTCGC601.3273511E-624.66666821
GCAGTCG952.764864E-1023.3684229
TAGAACA400.001925132723.1254
CCTTATT753.7059544E-722.227
CGCCTTA753.7059544E-722.225
CTAATCC502.6903063E-422.1999993
AGTCGGT1005.2568794E-1022.19999911
TAATCCT502.6903063E-422.1999994
TGATTCC1107.2759576E-1121.86363617
GGTGATT1059.677024E-1021.14285715
CAGTCGG1059.677024E-1021.14285710
TATACAC705.064041E-621.14285737
TGGGCAG1151.3278623E-1020.9130446
AGTCTGT450.003813929220.55555537
Produced by FastQC (version 0.11.5)