##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631875.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	185820
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58754170702831	34.0	31.0	34.0	31.0	34.0
2	32.756673124529115	34.0	31.0	34.0	31.0	34.0
3	32.84360133462491	34.0	31.0	34.0	31.0	34.0
4	36.281105370788936	37.0	37.0	37.0	35.0	37.0
5	36.19164244968249	37.0	35.0	37.0	35.0	37.0
6	36.24436551501453	37.0	37.0	37.0	35.0	37.0
7	36.23440964374125	37.0	37.0	37.0	35.0	37.0
8	36.21183941448714	37.0	36.0	37.0	35.0	37.0
9	38.05268001291572	39.0	38.0	39.0	35.0	39.0
10	37.96441179636207	39.0	38.0	39.0	35.0	39.0
11	38.06351307717146	39.0	38.0	39.0	35.0	39.0
12	37.99133570121623	39.0	38.0	39.0	35.0	39.0
13	38.04928963513077	39.0	38.0	39.0	35.0	39.0
14	39.42017005704445	40.0	39.0	41.0	37.0	41.0
15	39.42570767409321	40.0	39.0	41.0	37.0	41.0
16	39.37412011624152	40.0	39.0	41.0	36.0	41.0
17	39.34022171994403	40.0	39.0	41.0	36.0	41.0
18	39.37209665267463	40.0	39.0	41.0	36.0	41.0
19	39.39516736626843	40.0	39.0	41.0	37.0	41.0
20	39.38967818318803	40.0	39.0	41.0	37.0	41.0
21	39.3390216338392	40.0	39.0	41.0	36.0	41.0
22	39.31422882359272	40.0	39.0	41.0	36.0	41.0
23	39.256969109891294	40.0	39.0	41.0	36.0	41.0
24	39.275234097513724	40.0	39.0	41.0	36.0	41.0
25	39.218883866106985	40.0	39.0	41.0	36.0	41.0
26	39.161285114627056	40.0	39.0	41.0	36.0	41.0
27	39.04996771068776	40.0	39.0	41.0	35.0	41.0
28	39.03637391023571	40.0	39.0	41.0	35.0	41.0
29	39.00373479711549	40.0	39.0	41.0	35.0	41.0
30	38.95850285222258	40.0	38.0	41.0	35.0	41.0
31	38.88678828974276	40.0	38.0	41.0	35.0	41.0
32	38.8450812614358	40.0	38.0	41.0	35.0	41.0
33	38.77897427618125	40.0	38.0	41.0	35.0	41.0
34	38.75467656872242	40.0	38.0	41.0	35.0	41.0
35	38.69321386287805	40.0	38.0	41.0	35.0	41.0
36	38.65822301151652	40.0	38.0	41.0	35.0	41.0
37	38.60627488967818	40.0	38.0	41.0	35.0	41.0
38	38.5824453772468	40.0	38.0	41.0	35.0	41.0
39	38.50463889785814	40.0	38.0	41.0	34.0	41.0
40	38.1733559358519	40.0	38.0	41.0	34.0	41.0
41	38.24933806909913	40.0	37.0	41.0	34.0	41.0
42	38.25836831342159	40.0	37.0	41.0	34.0	41.0
43	37.79627058443655	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	2.0
19	2.0
20	11.0
21	23.0
22	27.0
23	54.0
24	92.0
25	171.0
26	276.0
27	355.0
28	612.0
29	832.0
30	1287.0
31	1768.0
32	2412.0
33	3181.0
34	4629.0
35	7031.0
36	10916.0
37	19409.0
38	45266.0
39	87463.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.32213970509095	16.53697126251211	13.183188031428264	27.957701000968676
2	20.665697987299538	19.526423420514476	32.687547088580345	27.120331503605637
3	20.81799591002045	20.023140673770314	29.373049187385643	29.785814228823593
4	15.425680766333011	16.005812076202776	34.53826283500162	34.0302443224626
5	16.737164998385534	32.6676353460338	33.959207835539765	16.6359918200409
6	33.57227424389194	34.083521687654724	15.769561941663977	16.574642126789367
7	30.349262727370572	27.621892153697125	20.220643633623936	21.808201485308363
8	27.27316758153051	30.5370788935529	20.668388763319342	21.521364761597244
9	28.64169626520289	12.706920675922936	17.837154235281456	40.81422882359272
10	19.09159401571413	23.516844257883974	30.06188784845549	27.329673877946398
11	38.75793778925842	19.466150037670864	18.81552039608223	22.960391776988484
12	22.80109783661608	24.15455817457755	26.662899580238943	26.38144440856743
13	35.2733828436121	17.752125713055644	20.763642234420406	26.210849208911853
14	24.0824453772468	20.625336347002477	23.331718867721452	31.960499408029275
15	29.61414271876009	24.24819718006673	20.047895813152515	26.089764288020667
16	25.146916370681303	24.173393606716175	22.681627381336778	27.99806264126574
17	26.896458938757938	23.746636529975245	21.82542245183511	27.531482079431708
18	26.718329566246908	21.247981917985147	23.387687008933376	28.646001506834573
19	28.058336024109355	22.3544290173286	23.472715531159185	26.11451942740286
20	30.269615757184372	20.899795501022496	22.7101496071467	26.120439134646432
21	27.52825314820794	22.253793994187923	21.83188031428264	28.386072543321493
22	28.554515122161234	22.823700355182435	21.31417500807233	27.30760951458401
23	27.922720912711224	22.088580346571952	22.469594230976213	27.51910450974061
24	27.646109137875364	21.578947368421055	23.249919276719407	27.52502421698418
25	27.70907329673878	22.051985792702617	23.151436874394577	27.087504036164027
26	27.72683241846949	22.831234528037886	22.476052093423743	26.965880960068883
27	26.869013023355937	21.88515767947476	24.123345172747822	27.12248412442148
28	26.10644709934345	22.89312237649338	22.334517274782048	28.66591324938112
29	26.89753524916586	23.5356796900226	22.70261543429125	26.86416962652029
30	26.913141750080722	22.274243891938436	23.61155957378108	27.201054784199762
31	27.821547734366593	22.54708858034657	22.78118609406953	26.850177591217307
32	25.38531912603595	22.450758798837583	23.01205467656872	29.151867398557744
33	25.524163168657843	22.10687762350662	25.01991174254655	27.34904746528899
34	26.41857711764073	21.767839845011302	24.18200408997955	27.631578947368425
35	25.588741793133142	23.59918200408998	24.729307932407707	26.082768270369172
36	25.842751049402647	22.680012915724895	25.01829727693467	26.458938757937787
37	26.358841890001077	22.504574319233665	24.506511677967925	26.630072112797333
38	25.62318372618663	21.68873103002906	25.449359595307286	27.23872564847702
39	25.219029168012053	20.657625659240125	26.522441072005165	27.600904100742653
40	24.84232052523948	21.68980734043698	27.31083844580777	26.157033688515767
41	23.234850931008502	20.727047680551074	28.336024109353136	27.70207727908729
42	21.829727693466797	21.064470993434504	29.3461414271876	27.7596598859111
43	20.834140566139276	21.81358303734797	29.234743299967707	28.117533096545046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	4.0
4	7.0
5	7.0
6	10.5
7	14.0
8	16.0
9	18.0
10	23.0
11	28.0
12	28.0
13	64.0
14	100.0
15	196.0
16	292.0
17	299.0
18	306.0
19	306.0
20	337.5
21	369.0
22	352.0
23	335.0
24	389.5
25	444.0
26	444.0
27	523.5
28	603.0
29	853.5
30	1104.0
31	1299.5
32	1495.0
33	1495.0
34	1868.0
35	2241.0
36	2595.5
37	2950.0
38	3634.0
39	4318.0
40	4318.0
41	5113.0
42	5908.0
43	6928.5
44	7949.0
45	11099.5
46	14250.0
47	14250.0
48	15772.0
49	17294.0
50	19357.0
51	21420.0
52	21882.5
53	22345.0
54	22345.0
55	20228.5
56	18112.0
57	17295.0
58	16478.0
59	15177.5
60	13877.0
61	13877.0
62	12511.0
63	11145.0
64	9207.0
65	7269.0
66	6256.0
67	5243.0
68	5243.0
69	4396.5
70	3550.0
71	3069.0
72	2588.0
73	2005.0
74	1422.0
75	1422.0
76	1171.5
77	921.0
78	748.0
79	575.0
80	481.0
81	387.0
82	387.0
83	304.5
84	222.0
85	166.0
86	110.0
87	93.5
88	77.0
89	77.0
90	56.5
91	36.0
92	24.5
93	13.0
94	8.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	185820.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.192103593109145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.88959628189991	49.693987917178504
2	6.105854573566613	6.98413334591762
3	2.064148619970325	3.5415900511474834
4	1.131299295547389	2.5880554602303074
5	0.7114675769863049	2.0345163683069556
6	0.5056144746610524	1.735029324779422
7	0.37571294032790997	1.504146938315382
8	0.2997709120737081	1.371562324601626
9	0.2248281798646096	1.1572556898120234
>10	1.486863692806902	16.042135503561585
>50	0.12290607137717569	4.812469205696677
>100	0.08093814456545716	8.241946429706386
>500	9.99236352659965E-4	0.29317144074603907
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	513	0.27607361963190186	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	499	0.2685394467764503	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	425	0.22871596168334948	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	417	0.22441072005166288	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	392	0.21095683995264233	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	373	0.2007318910773867	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	363	0.1953503390377785	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	341	0.1835109245506404	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	338	0.18189645893875792	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	300	0.16144656118824668	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	281	0.15122161231299105	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	275	0.1479926810892261	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	228	0.1226993865030675	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	226	0.12162307609514583	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	225	0.12108492089118503	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	219	0.11785598966742009	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	206	0.1108599720159294	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	199	0.10709288558820364	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	199	0.10709288558820364	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	199	0.10709288558820364	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	195	0.10494026477236035	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	192	0.10332579916047789	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	187	0.10063502314067378	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	186	0.10009686793671295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0010763104079216446	0.0
17	5.381552039608223E-4	0.0	0.0	0.0010763104079216446	0.0
18	5.381552039608223E-4	0.0	0.0	0.0016144656118824668	0.0
19	5.381552039608223E-4	0.0	0.0	0.0016144656118824668	0.0
20	5.381552039608223E-4	0.0	0.0	0.0021526208158432892	0.0
21	5.381552039608223E-4	0.0	0.0	0.0043052416316865785	0.0
22	5.381552039608223E-4	0.0	0.0	0.00699601765149069	0.0
23	5.381552039608223E-4	0.0	0.0	0.008072328059412335	0.0
24	5.381552039608223E-4	0.0	0.0	0.010763104079216447	0.0
25	5.381552039608223E-4	0.0	0.0	0.013453880099020557	0.0
26	5.381552039608223E-4	0.0	0.0	0.015068345710903024	0.0
27	5.381552039608223E-4	0.0	0.0	0.03013669142180605	0.0
28	5.381552039608223E-4	0.0	0.0	0.11139812721989022	0.0
29	5.381552039608223E-4	0.0	0.0	0.26154342912495965	0.0
30	5.381552039608223E-4	0.0	0.0	0.4423635776557959	0.0
31	5.381552039608223E-4	0.0	0.0	0.9611451942740287	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTAAG	30	3.587976E-4	30.833334	2
GGTATCA	265	0.0	30.018867	1
TTAGAAC	25	0.0054846387	29.599998	3
TAGATAA	25	0.0054846387	29.599998	4
GGTAGCG	25	0.0054846387	29.599998	8
TTAGGAC	35	8.8413234E-4	26.42857	3
TCCTCGC	60	1.3273511E-6	24.666668	21
GCAGTCG	95	2.764864E-10	23.368422	9
TAGAACA	40	0.0019251327	23.125	4
CCTTATT	75	3.7059544E-7	22.2	27
CGCCTTA	75	3.7059544E-7	22.2	25
CTAATCC	50	2.6903063E-4	22.199999	3
AGTCGGT	100	5.2568794E-10	22.199999	11
TAATCCT	50	2.6903063E-4	22.199999	4
TGATTCC	110	7.2759576E-11	21.863636	17
GGTGATT	105	9.677024E-10	21.142857	15
CAGTCGG	105	9.677024E-10	21.142857	10
TATACAC	70	5.064041E-6	21.142857	37
TGGGCAG	115	1.3278623E-10	20.913044	6
AGTCTGT	45	0.0038139292	20.555555	37
>>END_MODULE