FastQCFastQC Report
Fri 10 Feb 2017
ERR1631874.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631874.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664533
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11540.1736557853409838No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11520.17335482210815717No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10360.15589895460421077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.12505022323947795No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7770.11692421595315809No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7610.1145165100905448No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7490.11271073069358482No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7470.11240976746075815No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7290.10970109836531819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7130.10729339250270489No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6910.10398279694161162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.030.5251
ACCTAAC250.005494695629.68
CGATAAC250.005494695629.61
GGTAGAC358.865443E-426.428573
GTTCTAA502.7004402E-422.21
ATACGGC957.1304385E-921.42105329
TTCTGCG3950.021.07594918
GTAACGT3700.021.00000226
TTCTATA450.003824135820.5555552
TGCGGGT4050.020.55555521
ACTAGCA450.003824135820.5555552
GTAGACT450.003824135820.5555554
CGGGTAA3800.020.44736923
GGTAACG3800.020.44736925
GCGGTAA4850.020.21649623
AACGTCA3900.019.92307728
GTTTAGG656.8965295E-519.9230771
CTAAGTC656.8965295E-519.92307734
CGTCAAT4050.019.64197530
TTTAGGA951.673452E-719.4736842
Produced by FastQC (version 0.11.5)