##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631874.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	664533
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65849100044693	34.0	31.0	34.0	31.0	34.0
2	32.8212639552889	34.0	31.0	34.0	31.0	34.0
3	32.91679269502041	34.0	31.0	34.0	31.0	34.0
4	36.327792600216995	37.0	37.0	37.0	35.0	37.0
5	36.24856101954305	37.0	37.0	37.0	35.0	37.0
6	36.289591337074306	37.0	37.0	37.0	35.0	37.0
7	36.27701709320681	37.0	37.0	37.0	35.0	37.0
8	36.26014810400687	37.0	37.0	37.0	35.0	37.0
9	38.10063759060874	39.0	38.0	39.0	37.0	39.0
10	38.024405108549914	39.0	38.0	39.0	35.0	39.0
11	38.10277593437797	39.0	38.0	39.0	37.0	39.0
12	38.0360358326825	39.0	38.0	39.0	35.0	39.0
13	38.08923258890078	39.0	38.0	39.0	37.0	39.0
14	39.501138393428164	41.0	39.0	41.0	37.0	41.0
15	39.50457689836321	41.0	39.0	41.0	37.0	41.0
16	39.47009704559442	41.0	39.0	41.0	37.0	41.0
17	39.43108920098776	41.0	39.0	41.0	37.0	41.0
18	39.45112733302936	41.0	39.0	41.0	37.0	41.0
19	39.476719741532776	41.0	39.0	41.0	37.0	41.0
20	39.46334493546596	41.0	39.0	41.0	37.0	41.0
21	39.431796464584906	41.0	39.0	41.0	37.0	41.0
22	39.41176134217563	41.0	39.0	41.0	37.0	41.0
23	39.34948151559065	40.0	39.0	41.0	36.0	41.0
24	39.3624981754104	41.0	39.0	41.0	36.0	41.0
25	39.304176015337084	40.0	39.0	41.0	36.0	41.0
26	39.252201169844085	40.0	39.0	41.0	36.0	41.0
27	39.14175368266136	40.0	39.0	41.0	36.0	41.0
28	39.13657561024058	40.0	39.0	41.0	36.0	41.0
29	39.10488418182393	40.0	39.0	41.0	35.0	41.0
30	39.06752110128466	40.0	39.0	41.0	35.0	41.0
31	38.99554724897033	40.0	39.0	41.0	35.0	41.0
32	38.94265446561721	40.0	38.0	41.0	35.0	41.0
33	38.890903837732665	40.0	38.0	41.0	35.0	41.0
34	38.85387783601416	40.0	38.0	41.0	35.0	41.0
35	38.78716181137731	40.0	38.0	41.0	35.0	41.0
36	38.73330293604682	40.0	38.0	41.0	35.0	41.0
37	38.68728866737995	40.0	38.0	41.0	35.0	41.0
38	38.64166715573192	40.0	38.0	41.0	35.0	41.0
39	38.571727814871494	40.0	38.0	41.0	35.0	41.0
40	38.245075865306916	40.0	38.0	41.0	34.0	41.0
41	38.314679632162736	40.0	37.0	41.0	34.0	41.0
42	38.29294256267183	40.0	37.0	41.0	34.0	41.0
43	37.826335185762034	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	2.0
16	1.0
17	0.0
18	3.0
19	6.0
20	14.0
21	35.0
22	55.0
23	165.0
24	253.0
25	475.0
26	741.0
27	1298.0
28	1955.0
29	2883.0
30	4069.0
31	5918.0
32	7814.0
33	10763.0
34	15989.0
35	24268.0
36	37834.0
37	68455.0
38	155735.0
39	325800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73823421861668	16.465848949563078	11.966147655571657	27.829769176248583
2	20.550371463870114	19.086185336168406	33.002123295607596	27.361319904353888
3	20.86984393551562	19.829715002866674	28.4620929284174	30.838348133200306
4	15.297359198113563	15.083223857957393	33.63083548898249	35.988581454946555
5	17.30990033602545	33.18601183086468	32.64834101541985	16.85574681769002
6	35.7023654205284	33.600438202467	15.03296299807534	15.664233378929262
7	31.61799338783777	27.97829453164854	19.270374834658323	21.133337245855362
8	27.934203418039434	31.76742163293621	19.22011397477627	21.07826097424808
9	28.21951656275911	13.021475231478346	17.273032340004182	41.48597586575836
10	18.98160061276114	23.843812120692277	30.001218901092948	27.173368365453634
11	39.56929151750177	19.569080843238787	19.414536223182292	21.447091416077154
12	23.423516966049842	23.02940561266333	27.585988957658987	25.961088463627842
13	34.468265684322674	17.391461372121476	21.233558002386637	26.906714941169213
14	24.057496015999206	18.800119783366664	23.57971688388688	33.56266731674725
15	28.804288124141316	24.18826454066239	20.329313969358935	26.678133365837365
16	26.251818946538396	23.595667935226693	22.163684873437436	27.988828244797475
17	26.63178502798206	23.370848400305178	22.413935801532805	27.583430770179962
18	27.039740690078595	21.819382935083738	23.368591176058974	27.77228519877869
19	28.154207541235728	22.441473937336447	23.139859119110714	26.264459402317115
20	29.00382674750539	21.470867511470463	22.783067206594705	26.74223853442944
21	27.466356072610388	22.27684704898026	22.430037334489032	27.826759543920314
22	27.754979812891158	22.80985293431628	21.872502945677642	27.56266430711492
23	27.91373791820722	22.24012953457541	22.57901413473823	27.267118412479142
24	27.84451637465709	21.83232435409528	22.699098464636066	27.62406080661156
25	28.021031310709926	22.209130321594262	23.035424877319862	26.734413490375946
26	27.931494748943997	22.490229981054362	22.716102887290774	26.862172382710863
27	27.41534280464627	22.166243060916464	23.39221678983587	27.0261973446014
28	26.937413190917532	23.00186747685969	22.36487879458206	27.69584053764072
29	27.260497221356957	23.28823399289426	22.36728650044467	27.083982285304113
30	26.961941694392905	22.6893171595692	23.302981191302766	27.045759954735132
31	27.656715317373255	22.380378401072633	22.472172187084766	27.49073409446935
32	25.79209760839567	22.614979241061015	23.19237720323897	28.400545947304344
33	26.352190184686087	22.379625992990565	24.147784985847203	27.120398836476138
34	26.52313730093163	22.09837585191405	23.77534298522421	27.603143861930107
35	26.239930898841745	23.277098353279673	23.928834233965805	26.55413651391278
36	26.44759552949214	22.45757547029267	24.268621723827106	26.826207276388082
37	26.91845250724945	21.99213583072624	23.77925550725096	27.310156154773352
38	25.870649012163426	21.867537052336	24.699149628385648	27.56266430711492
39	25.30333331828517	21.12084727169305	25.622956271547086	27.952863138474687
40	25.50031375417022	21.768670630352442	25.936409478536053	26.79460613694128
41	24.718862720135796	21.071188338276656	26.73035048673279	27.479598454854763
42	23.77173142643029	21.442125522735513	27.578013431989078	27.208129618845113
43	23.20546910386693	21.48155170623581	27.545509402843805	27.767469787053468
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	8.5
2	7.0
3	27.5
4	48.0
5	48.0
6	58.5
7	69.0
8	76.5
9	84.0
10	121.5
11	159.0
12	159.0
13	305.0
14	451.0
15	936.5
16	1422.0
17	1419.0
18	1416.0
19	1416.0
20	1565.5
21	1715.0
22	1628.0
23	1541.0
24	1805.5
25	2070.0
26	2070.0
27	2505.0
28	2940.0
29	3784.5
30	4629.0
31	5616.5
32	6604.0
33	6604.0
34	8322.5
35	10041.0
36	11477.5
37	12914.0
38	15238.0
39	17562.0
40	17562.0
41	20799.5
42	24037.0
43	27591.0
44	31145.0
45	38204.0
46	45263.0
47	45263.0
48	49355.0
49	53447.0
50	57385.5
51	61324.0
52	62734.5
53	64145.0
54	64145.0
55	62327.0
56	60509.0
57	58812.0
58	57115.0
59	54959.5
60	52804.0
61	52804.0
62	48583.0
63	44362.0
64	38502.0
65	32642.0
66	28932.0
67	25222.0
68	25222.0
69	21550.0
70	17878.0
71	15078.5
72	12279.0
73	9974.0
74	7669.0
75	7669.0
76	6222.5
77	4776.0
78	3739.0
79	2702.0
80	2196.5
81	1691.0
82	1691.0
83	1350.5
84	1010.0
85	754.0
86	498.0
87	357.5
88	217.0
89	217.0
90	156.0
91	95.0
92	56.5
93	18.0
94	10.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	664533.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.00952730275884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.39945807048188	55.06998539538355
2	7.014115337379509	8.839121837106275
3	2.1286007380931666	4.02366378970662
4	0.9969202053259909	2.512618835846399
5	0.5581489486477098	1.7584350709412004
6	0.3571564318708602	1.3502554767193737
7	0.24332569163858656	1.0732285767534937
8	0.18970753657940786	0.9562705764511457
9	0.14362397088557685	0.8144710663360856
>10	0.8228916400430879	9.797406396569066
>50	0.07770230557580152	3.373242016868712
>100	0.06235358633125662	7.827064592629
>500	0.0052760726895396855	2.0992254632603284
>1k	7.194644576645025E-4	0.5050109054287372
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1154	0.1736557853409838	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1152	0.17335482210815717	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1036	0.15589895460421077	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.12505022323947795	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	777	0.11692421595315809	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	761	0.1145165100905448	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	749	0.11271073069358482	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	747	0.11240976746075815	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	729	0.10970109836531819	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	713	0.10729339250270489	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	691	0.10398279694161162	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5048161641333086E-4	0.0
2	0.0	0.0	0.0	1.5048161641333086E-4	0.0
3	0.0	0.0	0.0	1.5048161641333086E-4	0.0
4	0.0	0.0	0.0	1.5048161641333086E-4	0.0
5	0.0	0.0	0.0	1.5048161641333086E-4	0.0
6	0.0	0.0	0.0	1.5048161641333086E-4	0.0
7	0.0	0.0	0.0	1.5048161641333086E-4	0.0
8	1.5048161641333086E-4	0.0	0.0	1.5048161641333086E-4	0.0
9	1.5048161641333086E-4	0.0	0.0	1.5048161641333086E-4	0.0
10	1.5048161641333086E-4	0.0	0.0	1.5048161641333086E-4	0.0
11	1.5048161641333086E-4	0.0	0.0	1.5048161641333086E-4	0.0
12	1.5048161641333086E-4	0.0	0.0	1.5048161641333086E-4	0.0
13	1.5048161641333086E-4	0.0	0.0	3.009632328266617E-4	0.0
14	1.5048161641333086E-4	0.0	0.0	3.009632328266617E-4	0.0
15	3.009632328266617E-4	0.0	0.0	3.009632328266617E-4	0.0
16	3.009632328266617E-4	0.0	0.0	6.019264656533234E-4	0.0
17	3.009632328266617E-4	0.0	0.0	9.028896984799851E-4	0.0
18	4.514448492399926E-4	0.0	0.0	0.0012038529313066469	0.0
19	4.514448492399926E-4	0.0	0.0	0.0015048161641333087	0.0
20	4.514448492399926E-4	0.0	0.0	0.002257224246199963	0.0
21	4.514448492399926E-4	0.0	0.0	0.004363966875986595	0.0
22	4.514448492399926E-4	0.0	0.0	0.007223117587839881	0.0
23	4.514448492399926E-4	0.0	0.0	0.009028896984799853	0.0
24	4.514448492399926E-4	0.0	0.0	0.011135639614586483	0.0
25	4.514448492399926E-4	0.0	0.0	0.011888047696653138	0.0
26	4.514448492399926E-4	0.0	0.0	0.012790937395133123	0.0
27	4.514448492399926E-4	0.0	0.0	0.025431393173852915	0.0
28	4.514448492399926E-4	0.0	0.0	0.08171151771243866	0.0
29	4.514448492399926E-4	0.0	0.0	0.183286608791437	0.0
30	4.514448492399926E-4	0.0	0.0	0.306079607784715	0.0
31	4.514448492399926E-4	0.0	0.0	0.731340655768788	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	600	0.0	30.525	1
ACCTAAC	25	0.0054946956	29.6	8
CGATAAC	25	0.0054946956	29.6	1
GGTAGAC	35	8.865443E-4	26.42857	3
GTTCTAA	50	2.7004402E-4	22.2	1
ATACGGC	95	7.1304385E-9	21.421053	29
TTCTGCG	395	0.0	21.075949	18
GTAACGT	370	0.0	21.000002	26
TTCTATA	45	0.0038241358	20.555555	2
TGCGGGT	405	0.0	20.555555	21
ACTAGCA	45	0.0038241358	20.555555	2
GTAGACT	45	0.0038241358	20.555555	4
CGGGTAA	380	0.0	20.447369	23
GGTAACG	380	0.0	20.447369	25
GCGGTAA	485	0.0	20.216496	23
AACGTCA	390	0.0	19.923077	28
GTTTAGG	65	6.8965295E-5	19.923077	1
CTAAGTC	65	6.8965295E-5	19.923077	34
CGTCAAT	405	0.0	19.641975	30
TTTAGGA	95	1.673452E-7	19.473684	2
>>END_MODULE