FastQCFastQC Report
Fri 10 Feb 2017
ERR1631873.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631873.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8403
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA470.5593240509341901No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA420.49982149232416995No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA410.4879209806021659No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA370.4403189337141497No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC340.40461739854813755No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT340.40461739854813755No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG340.40461739854813755No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC330.39271688682613354No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA320.38081637510412947No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT300.3570153516601214No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA290.3451148399381173No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT280.3332143282161133No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT270.32131381649410923No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA260.3094133047721052No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA230.27371176960609306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230.27371176960609306No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA200.23801023444008093No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA200.23801023444008093No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT190.22610972271807686No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA190.22610972271807686No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC190.22610972271807686No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA190.22610972271807686No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC190.22610972271807686No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG170.20230869927406878No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA170.20230869927406878No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG160.19040818755206473No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT160.19040818755206473No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA160.19040818755206473No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC160.19040818755206473No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA150.1785076758300607No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA150.1785076758300607No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA150.1785076758300607No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA150.1785076758300607No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC140.16660716410805665No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT140.16660716410805665RNA PCR Primer, Index 27 (95% over 23bp)
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA140.16660716410805665No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT130.1547066523860526No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC130.1547066523860526No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA130.1547066523860526No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC130.1547066523860526No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC130.1547066523860526No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT130.1547066523860526No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC130.1547066523860526No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA130.1547066523860526No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC130.1547066523860526No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG130.1547066523860526No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC120.14280614066404856No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG120.14280614066404856No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA120.14280614066404856No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG120.14280614066404856No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT120.14280614066404856No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA120.14280614066404856No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA120.14280614066404856No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG110.13090562894204452No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG110.13090562894204452No Hit
CTCCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCA110.13090562894204452No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT110.13090562894204452No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC110.13090562894204452No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG110.13090562894204452No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC110.13090562894204452No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT110.13090562894204452No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC110.13090562894204452No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG110.13090562894204452No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT110.13090562894204452No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT110.13090562894204452No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC100.11900511722004047No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG100.11900511722004047No Hit
CTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTA100.11900511722004047No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC100.11900511722004047No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG100.11900511722004047No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG100.11900511722004047No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC100.11900511722004047No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC100.11900511722004047No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC100.11900511722004047No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC90.10710460549803642No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG90.10710460549803642No Hit
TTAGTGGACCATGAAGTGCGATTGCCTCGGTGATAACGACATT90.10710460549803642No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA90.10710460549803642No Hit
TCGCAGATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCA90.10710460549803642No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC90.10710460549803642No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT90.10710460549803642No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA90.10710460549803642No Hit
GGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAAC90.10710460549803642No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA90.10710460549803642No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG90.10710460549803642No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)